本文關(guān)鍵詞：逍遙中空栓對肝郁型大鼠行為學(xué)以及性激素的藥效學(xué)研究　出處：《山西省中醫(yī)藥研究院》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 逍遙散 中空栓 肝郁 行為學(xué)

【摘要】：1.研究目的本實驗旨在從動物行為學(xué)以及性激素E2、T、PRL含量兩方面對逍遙散免煎顆粒劑以及逍遙中空栓進行治療效果的對比,探討在中醫(yī)傳統(tǒng)理論指導(dǎo)下兩種劑型對肝郁證大鼠的治療作用,為臨床合理使用逍遙散提供支持。2.研究方法用1月齡雌性SD大鼠50只,隨機分為5組:空白對照組、肝郁模型組、逍遙散免煎顆粒劑組、逍遙中空栓高劑量組、逍遙中空栓低劑量組,每組10只,通過肝郁慢性不可預(yù)知的多種應(yīng)激刺激隨機組合法復(fù)制肝郁模型,21天后每組做陰道涂片連續(xù)5天,與此同時做行為學(xué)檢測,逍遙中空栓高、低劑量組塞肛納藥;逍遙散免煎顆粒劑組灌胃,連續(xù)給藥10天,給藥結(jié)束后當日繼續(xù)做陰道涂片5天,與此同時做行為學(xué)檢測,結(jié)束后于第二天處死動物,取血備用。3.研究內(nèi)容3.1一般狀態(tài)、體重、攝食實驗開始后,每天觀察大鼠的一般狀態(tài),大鼠體重增長情況、攝食量每3天記錄一次。3.2陰道涂片檢測分別于造模后、給藥后做大鼠陰道涂片檢測,連續(xù)5天,用顯微鏡觀察各大鼠動情周期是否紊亂。3.3行為學(xué)檢測分別于造模后、給藥后做曠場實驗、高架十字迷宮實驗、糖水偏嗜實驗,檢驗造模是否成功以及給藥是否有效。3.4性激素檢測將腹主動脈所取的血離心,分離血清后,放射免疫分析法測定血清E2、T、PRL的含量。3.5統(tǒng)計學(xué)采用SPSS17.0統(tǒng)計軟件進行分析,計量資料以均數(shù)±標準差表示,組間比較用獨立樣本t檢驗,組內(nèi)比較用配對t檢驗,定量資料組間比較采用方差分析,P0.05為差異有統(tǒng)計學(xué)意義。4.結(jié)果4.1造模成功判定:與空白對照組比較,造模后肝郁模型組、逍遙散免煎顆粒劑組、逍遙中空栓高劑量組、逍遙中空栓低劑量組的一般狀態(tài)、體重、攝食以及行為學(xué)的均值上觀察有顯著變化,經(jīng)t檢驗分析,P0.05差異有統(tǒng)計學(xué)意義,提示造模成功。4.2各組陰道涂片的觀察:造模后空白對照組5天涂片中動情前期、動情期、動情間期、靜止期規(guī)律交替出現(xiàn),僅有1天鏡下可見典型角化細胞增多;肝郁模型組鏡下5天中會有3-5天連續(xù)出現(xiàn)角化細胞,逍遙散免煎顆粒劑組、逍遙中空栓高劑量組、逍遙中空栓低劑量組給藥后鏡下5天中角化細胞有規(guī)律出現(xiàn),部分已經(jīng)恢復(fù)正常動情周期。4.3通過對行為學(xué)數(shù)據(jù)分析發(fā)現(xiàn):曠場實驗中,從外周格、中央格穿越格數(shù)上,逍遙散免煎顆粒劑組、逍遙中空栓高劑量組、逍遙中空栓低劑量組與肝郁模型組相比P0.05,有統(tǒng)計學(xué)意義;從站立次數(shù)以及理毛修飾次數(shù)上來說,逍遙中空栓高劑量組P0.05,有統(tǒng)計學(xué)意義;高架十字迷宮實驗中,從開臂時間百分比上,逍遙散免煎顆粒劑組、逍遙中空栓高劑量組、逍遙中空栓低劑量組與肝郁模型組相比P0.05,有統(tǒng)計學(xué)意義;糖水偏嗜實驗中,逍遙散免煎顆粒劑組、逍遙中空栓高劑量組、逍遙中空栓低劑量組糖水偏嗜度與肝郁模型組相比P0.05,有統(tǒng)計學(xué)意義;其余組間比較經(jīng)統(tǒng)計學(xué)分析P0.05,認為無差異。4.4通過對性激素E2、T、PRL檢測結(jié)果分析:逍遙散免煎顆粒劑組、逍遙中空栓高劑量組T、PRL與肝郁模型組相比P0.05,有統(tǒng)計學(xué)意義;逍遙中空栓低劑量組PRL與肝郁模型組相比P0.05,有統(tǒng)計學(xué)意義;其余組間比較經(jīng)統(tǒng)計學(xué)分析P0.05,認為無差異。5.結(jié)論5.1通過對大鼠進行21天肝郁慢性不可預(yù)知的多種應(yīng)激刺激隨機組合法,可以成功復(fù)制出肝郁證模型。5.2肝郁模型的雌性大鼠一般狀態(tài)差,體重下降,攝食減少,動情周期紊亂,曠場實驗中水平和垂直得分降低,高架十字迷宮實驗中開臂停留時間及次數(shù)比例下降,糖水偏嗜度降低。5.3逍遙中空栓可改善肝郁證雌性大鼠的一般狀態(tài)、增加體重、攝食增多,上調(diào)曠場實驗中水平和垂直得分、高架十字迷宮實驗中開臂停留時間及次數(shù)比例、糖水偏嗜程度以及改善紊亂的動情周期、升高血清E2、PRL含量、降低血清T含量,與逍遙散免煎顆粒劑比較無差異,可以作為一種新劑型應(yīng)用于臨床。
[Abstract]:1 . The aim of this study was to compare the therapeutic effects of Xiaoyao Powder and Xiaoyao hollow suppository from the aspects of animal behavior and sex hormone E2 , T and PRL levels . The rats were randomly divided into five groups : blank control group , liver depression model group , Xiaoyao powder free decoction granule group , Xiaoyao hollow suppository high dose group and Xiaoyao hollow suppository low dose group . Compared with the blank control group , there were significant changes in the normal state , body weight , food consumption and the mean value of Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dosage group , Xiaoyao hollow suppository high dose group , Xiaoyao hollow suppository high dosage group , the low dose group of Xiaoyao hollow suppository has significant difference compared with the liver depression model group , there is no difference . 4.4 Through analysis of sex hormone E2 , T , PRL detection result : Conclusion : Compared with the liver - qi model group , there is no difference in the time and frequency proportion of open arms , the increase of serum E2 and PRL levels , the decrease of serum T content , the increase of serum E2 and PRL levels , the decrease of serum T content , and the decrease of serum T content .

【學(xué)位授予單位】：山西省中醫(yī)藥研究院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R285.5;R-332

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