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  本文關鍵詞：IL-17和P38信號通路在三叉神經(jīng)痛ION-CCI模型大鼠中的研究　出處：《安徽醫(yī)科大學》2017年碩士論文　論文類型：學位論文

  更多相關文章： 三叉神經(jīng)痛 眶下神經(jīng)慢性縮窄術 三叉神經(jīng)節(jié) IL-17

【摘要】：目的選用成年雄性SD大鼠,經(jīng)眶下神經(jīng)慢性縮窄術建立大鼠三叉神經(jīng)痛模型,通過行為學實驗驗證模型是否建立成功、Western blot實驗檢測三叉神經(jīng)節(jié)內IL-17和P-P38蛋白的表達量、RT-PCR實驗檢測三叉神經(jīng)節(jié)內IL-17A、CXCL1、CXCL2的m RNA表達量,探討IL-17和P38信號通路在ION-CCI模型大鼠中作用機制。方法將雄性SD大鼠隨機分為兩組,ION-CCI組:成年雄性SD大鼠,經(jīng)顴骨下緣入路,行眶下神經(jīng)慢性縮窄術;sham組:單純暴露眶下神經(jīng)而不結扎。分別在術前、術后1 w、術后2 w、術后3 w對兩組大鼠的觸須墊機械痛閾進行測定,記錄結果并比較,確定大鼠TN模型是否建立成功;提取三叉神經(jīng)節(jié)組織總蛋白,采用Western blot實驗方法檢測三叉神經(jīng)節(jié)內IL-17和P-P38的表達量,根據(jù)實驗結果條帶的灰度值進行半定量分析比較目的蛋白的表達量變化;提取三叉神經(jīng)節(jié)組織總m RNA,利用RT-PCR實驗檢測IL-17A、CXCL1和CXCL2的m RNA相對表達量;所有實驗數(shù)據(jù)均采用SPSS17.0軟件進行統(tǒng)計學分析。結果與sham組相比,ION-CCI組大鼠的機械痛閾明顯降低,并在2 w時痛閾達到最低(sham組:14.11±0.81 g vs ION-CCI組:4.45±0.92 g,n=5,P0.01)。與建模前相比,ION-CCI組大鼠的TG在建模后1 w、2 w、3 w時IL-17和P-P38的蛋白表達量均明顯增高,并在2 w時表達量達到峰值,3 w時有所下降,但仍高于建模前,且差異有統(tǒng)計學意義(n=3,P0.05)。在建模后2 w時,與sham組相比,ION-CCI組大鼠的TG中,IL-17及相關趨化因子(CXCL1、CXCL2)的m RNA表達量明顯升高,且IL-17A組差異有統(tǒng)計學意義(n=3,P0.05)。結論IL-17與三叉神經(jīng)痛的發(fā)生密切相關;IL-17可能是通過P38信號通路參與三叉神經(jīng)痛的發(fā)生。
[Abstract]:Objective to select the adult male SD rats with chronic constriction of the infraorbital nerve to establish a rat model of trigeminal neuralgia, through the behavioral experiment model is established successfully, the expression of Western was detected by blot assay and P-P38 protein IL-17 in the trigeminal ganglion, trigeminal ganglion RT-PCR assay of IL-17A, CXCL1, expression of M RNA CXCL2 the role of IL-17 and P38 signaling pathway in ION-CCI rats. Methods male SD rats were randomly divided into two groups, group ION-CCI: adult male SD rats by the zygomatic margin approach for infraorbital nerve chronic constriction; group sham: simple and not exposed infraorbital nerve ligation. Respectively in preoperative, postoperative 1 W, 2 W after operation, 3 W after the operation of two groups of rat vibrissa pad mechanical pain threshold were measured and recorded the results and comparison, determine the TN rat model is established successfully; extraction of trigeminal ganglion tissue total protein by Western blot. The expression assay in the trigeminal ganglion of IL-17 and P-P38, according to the experimental results with the gray value of the semi quantitative analysis of the variation of the expression of target protein is extracted; trigeminal ganglion tissue total m RNA, using RT-PCR IL-17A m RNA CXCL1 assay, and the expression of CXCL2; all of the experimental data by SPSS17.0 the software for statistical analysis. Results compared with sham group, the mechanical pain threshold of rats in ION-CCI group were significantly decreased, and at 2 W reached the lowest threshold (Group sham: 14.11 + 0.81 g vs ION-CCI group: 4.45 + 0.92 g, n=5, P0.01). Compared with before modeling, the rats of group ION-CCI in TG modeling after 1 W, 2 W, 3 W IL-17 and P-P38 protein expression were significantly increased, and in 2 w expression reached the peak at 3 W decreased, but still higher than that before modeling, and the difference was statistically significant (n=3, P0.05). In 2 W after modeling, compared with sham group, ION-C The rats of group CI in TG, IL-17 and related chemokines (CXCL1, CXCL2) m RNA expression was significantly increased, and there was significant difference in group IL-17A (n=3, P0.05). Conclusion IL-17 and trigeminal neuralgia is closely related to the occurrence of; IL-17 may be through P38 pathway in trigeminal neuralgia.

【學位授予單位】：安徽醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R745.11;R-332

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