【摘要】：目的探討尼古丁對大鼠氣道平滑肌細胞增殖的作用。方法 (1)不同濃度的尼古丁作用大鼠氣道平滑肌細胞24、48 h,應用細胞計數(shù)法檢測大鼠氣道平滑肌細胞的增殖變化;(2)不同濃度的尼古丁作用大鼠氣道平滑肌細胞24 h,應用流式細胞術(shù)檢測細胞周期變化;(3)1×10-5mol/L尼古丁分別作用大鼠氣道平滑肌細胞12、16、20、24 h,Western blot檢測Cyclin D1的表達水平。結(jié)果 (1)尼古丁組細胞計數(shù)均明顯高于對照組,且尼古丁的促增殖作用與濃度和作用時間具有相關(guān)性。1×10-4、1×10-5、1×10-6、1×10-7mol/L尼古丁作用24 h,以對照組細胞計數(shù)值為1,則尼古丁組細胞計數(shù)分別為1.38、1.41、1.27、1.30,差異均有統(tǒng)計學意義(P0.05);尼古丁作用48 h,尼古丁組細胞計數(shù)分別為1.53、1.54、1.32、1.37,差異均有統(tǒng)計學意義(P0.05)。(2)各濃度尼古丁組S期的細胞分數(shù)均比對照組明顯升高,差異有統(tǒng)計學意義(P0.05)。以對照組S期的細胞分數(shù)值為1,1×10-7、1×10-6、1×10-5、1×10-4mol/L尼古丁作用24 h,尼古丁組S期的細胞分數(shù)分別為1.53、2.35、3.83、2.86,差異均有統(tǒng)計學意義(P0.05)。(3)1×10-5mol/L尼古丁作用細胞12、16、20、24 h后,在12、20 h大鼠氣道平滑肌細胞的Cyclin D1表達水平明顯高于對照組,差異有統(tǒng)計學意義(P0.05)。結(jié)論尼古丁能夠通過上調(diào)Cyclin D1促進大鼠氣道平滑肌細胞的增殖。
[Abstract]:Objective to investigate the effect of nicotine on proliferation of rat airway smooth muscle cells. Methods (1) the proliferation of rat airway smooth muscle cells (ASMCs) was detected by cell count method at 24 h after exposure to different concentrations of nicotine, (2) the rat airway smooth muscle cells were treated with different concentrations of nicotine for 24 h. The changes of cell cycle were detected by flow cytometry. (3) 1 脳 10-5mol/L nicotine was used to detect the expression of Cyclin D1 in rat airway smooth muscle cells. Results (1) the cell count in nicotine group was significantly higher than that in control group. Moreover, the effect of nicotine on proliferation was correlated with the concentration and time of action. 1 脳 10-4, 1 脳 10-5, 1 脳 10-6, 1 脳 10-6, 1 脳 10-7mol/L for 24 h, and the cell count of control group was 1, the cell count in nicotine group was 1.38 1 1 1. 27 1. 30, the difference was statistically significant (P0.05); the effect of nicotine on nicotine was significant (P0.05). At 48 h, the cell counts in nicotine group were 1.53n 1.54U 1.32U 1.37.The difference was statistically significant (P0.05). (2). The cell fractions of S-phase in nicotine group were significantly higher than those in control group. The difference was statistically significant (P0.05). The cell fraction of S phase in control group was 1 脳 10 ~ (-7) C ~ (-1) 脳 10 ~ (-6) C ~ (-1) 脳 10 ~ (-5) N ~ (-1) 10-4mol/L nicotine for 24 h, and the cell fraction in S phase of nicotine group was 1.53C ~ (2.35) C ~ (3) 3.83 ~ (3) C ~ (-1) 2.86, respectively. The difference was significant (P0.05). (3) 1 脳 10-5mol/L nicotine treated cells for 24 h. The expression of Cyclin D1 in airway smooth muscle cells was significantly higher than that in the control group at 1220 h (P0.05). Conclusion nicotine can promote the proliferation of rat airway smooth muscle cells by up-regulating Cyclin D1.
【作者單位】： 廣州醫(yī)科大學形態(tài)學實驗中心;廣州醫(yī)科大學附屬第一醫(yī)院、呼吸疾病國家重點實驗室;
【基金】：廣州醫(yī)科大學博士、留學回國人員科研項目(編號:2013C09)
【分類號】：R563.9

【共引文獻】

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