本文選題：煙曲霉菌 + 支氣管上皮細(xì)胞　； 參考：《山東大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2014年11期

【摘要】：目的探討煙曲霉菌對(duì)呼吸道上皮細(xì)胞修復(fù)功能的影響及其可能機(jī)制。方法實(shí)驗(yàn)分為正常對(duì)照組、不同濃度(10、20、40 mg/L)AFE組、熱處理AFE組。分別應(yīng)用不同濃度和熱處理的煙曲霉菌提取物(aspergillus fumigatus extract,AFE)作用體外培養(yǎng)的人支氣管上皮細(xì)胞16HBE不同的時(shí)間,以細(xì)胞體外損傷修復(fù)功能、細(xì)胞遷移能力、定量細(xì)胞黏附能力、細(xì)胞伸展面積為指標(biāo),觀察AFE和熱處理的AFE對(duì)上皮細(xì)胞修復(fù)能力的影響。結(jié)果20、40 mg/L AFE均明顯抑制16HBE細(xì)胞的體外損傷修復(fù)功能、細(xì)胞遷移能力和細(xì)胞黏附能力(P0.05),并呈明顯的時(shí)間和濃度依賴性。10 mg/L AFE與正常對(duì)照組差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。10、20、40 mg/L AFE均明顯抑制細(xì)胞的伸展能力(P0.05),并呈明顯濃度依賴性。另外,熱處理AFE的蛋白酶完全滅活,對(duì)上述觀察指標(biāo)無(wú)明顯影響(P0.05)。結(jié)論 AFE依賴其蛋白酶活性明顯抑制細(xì)胞的修復(fù)功能,并呈明顯的濃度和時(shí)間依賴性。
[Abstract]:Objective to investigate the effect of Aspergillus fumigatus on the repair function of respiratory epithelial cells and its possible mechanism. Methods the experiment was divided into normal control group, AFE group with different concentration (10 ~ 20 mg / L) and heat treated AFE group. Different concentrations and heat treatment of Aspergillus fumigatus extract (aspergillus fumigatus extract AFE) were used to treat 16HBE of human bronchial epithelial cells cultured in vitro for different time. The cell damage repair function, cell migration ability and cell adhesion ability were measured in vitro. The effects of AFE and heat treated AFE on the repair ability of epithelial cells were observed. Results 40 mg / L AFE significantly inhibited the repair function of 16HBE cells in vitro. The cell migration ability and cell adhesion ability (P0.05) were significantly time-and concentration-dependent. 10 mg / L AFE had no significant difference compared with the normal control group (P0.05). 10 mg / L AFE significantly inhibited the cell extension (P0.05), and in a dose-dependent manner. In addition, the protease of heat treatment AFE was completely inactivated, which had no significant effect on the above indexes (P0.05). Conclusion AFE dependent protease activity significantly inhibited the repair function of the cells in a concentration and time dependent manner.
【作者單位】： 山東大學(xué)附屬省立醫(yī)院呼吸科;復(fù)旦大學(xué)附屬華山醫(yī)院呼吸科;
【基金】：山東省自然科學(xué)基金(ZR 2010HM104)
【分類號(hào)】：R562.25

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 高福生;龍飛;祁卉卉;張軼;金先橋;;吸入煙曲霉孢子對(duì)哮喘大鼠氣道上皮重構(gòu)和MUC5AC表達(dá)的影響[J];上海交通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2008年09期

【共引文獻(xiàn)】

相關(guān)期刊論文 前2條

1 陳萍;陳祥貴;鄧茂先;;真菌與哮喘[J];青島醫(yī)藥衛(wèi)生;2008年03期

2 高福生;龍飛;祁卉卉;張軼;金先橋;;吸入煙曲霉孢子對(duì)哮喘大鼠氣道上皮重構(gòu)和MUC5AC表達(dá)的影響[J];上海交通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2008年09期

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