本文關(guān)鍵詞：氫硫酸鈉抑制脂多糖引起的A549細(xì)胞損傷　出處：《細(xì)胞與分子免疫學(xué)雜志》2016年04期 　論文類型：期刊論文

  更多相關(guān)文章： 急性肺損傷 脂多糖 氫硫酸鈉(Na HS) 肺上皮細(xì)胞 閉鎖小帶蛋白

【摘要】：目的觀察氫硫酸鈉(Na HS)對(duì)脂多糖(LPS)誘導(dǎo)的A549肺癌細(xì)胞損傷的影響并探討其可能機(jī)制。方法 A549細(xì)胞分為對(duì)照組、LPS處理組、Na HS處理組和LPS聯(lián)合Na HS處理組。處理24 h后,采用ELISA測(cè)定培養(yǎng)液中C反應(yīng)蛋白(CRP)的含量;MTT法與乳酸脫氫酶(LDH)法測(cè)定細(xì)胞損傷情況;測(cè)定A549細(xì)胞單層模型的跨上皮電阻(TER)值;免疫熒光染色法測(cè)定A549細(xì)胞中閉鎖小帶蛋白1(ZO-1)的分布情況。結(jié)果對(duì)照組與Na HS處理組比較,CRP的水平、TER值、細(xì)胞活力和ZO-1表達(dá)與分布情況無(wú)顯著性差異;與對(duì)照組相比,LPS處理組中的CRP水平明顯升高、TER值顯著減少、細(xì)胞活力下降、細(xì)胞膜ZO-1表達(dá)減少且邊界不清;與LPS處理組相比,LPS聯(lián)合Na HS處理組中的CRP水平明顯降低、TER值增加、細(xì)胞活力增加,ZO-1在細(xì)胞膜處的表達(dá)增強(qiáng)且分布情況部分恢復(fù)。結(jié)論 Na HS通過(guò)增強(qiáng)A549細(xì)胞活力、降低CRP水平、增加TER值并增加ZO-1表達(dá)抑制LPS誘導(dǎo)的肺損傷。
[Abstract]:Objective To observe the effect of sodium hydrogen sulfate (Na HS) on lipopolysaccharide (LPS) induced A549 damage of lung cancer cells and to explore its possible mechanism. Methods A549 cells were divided into control group, LPS treatment group, Na HS treatment group and LPS combined with Na HS group. After 24 h, determination of C reactive protein in culture using ELISA (CRP) content; MTT and lactate dehydrogenase (LDH) method for the determination of cell injury; transepithelial electrical resistance determination of A549 cell monolayer model (TER); Determination of A549 cell zonula occludens protein 1 immunofluorescence staining method (ZO-1). The distribution of the results of control group group compared with the Na HS treatment, CRP level, TER value, cell viability and ZO-1 expression had no significant difference with the distribution; compared with the control group, LPS treatment group, CRP levels were significantly increased, TER value decreased significantly, decreased cell viability, cell membrane expression of ZO-1 decreased and the boundary is not clear; compared with LPS group, LPS Lian Na HS in the CRP group were significantly decreased, TER value increased, the cell viability increased, the expression of ZO-1 in cell membrane enhanced recovery and distribution part. Conclusion Na HS enhances A549 cell viability, decrease the level of CRP, increase TER value and increase of ZO-1 lung injury induced suppression of LPS expression.

【作者單位】： 河北省人民醫(yī)院感染性疾病科;河北省人民醫(yī)院感染性疾病科胸外科公費(fèi)醫(yī)保辦公室;天津醫(yī)科大學(xué)總醫(yī)院麻醉科;
【基金】：河北省醫(yī)學(xué)科學(xué)研究重點(diǎn)課題計(jì)劃(ZL20140267)
【分類號(hào)】：R563
【正文快照】： 急性肺損傷(acute lung injury,ALI)是指在感染、休克等致傷因素下,肺毛細(xì)血管內(nèi)皮細(xì)胞和肺泡上皮細(xì)胞損傷造成彌漫性肺間質(zhì)及肺泡水腫,這些變化導(dǎo)致肺容積減少、肺順應(yīng)性降低、嚴(yán)重的通氣/血流比例失調(diào),最終可引起急性低氧性呼吸功能不全[1-3]。目前,ALI的病死率大約為35%~40，

本文編號(hào)：1436468