本文關(guān)鍵詞：結(jié)核分枝桿菌抗原特異性白細(xì)胞介素-22在結(jié)核性胸膜炎鑒別診斷中的作用研究　出處：《中國(guó)人民解放軍醫(yī)學(xué)院》2014年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 結(jié)核桿菌特異性白細(xì)胞介素-22 結(jié)核性胸水 惡性胸水 鑒別診斷

【摘要】：目的:通過分析結(jié)核性與惡性胸液?jiǎn)蝹�(gè)核細(xì)胞（Pleural fluid mononuclear cells,PFMCs）在受到結(jié)核分枝桿菌特異性抗原肽ESAT-6（early secreted antigenic target-6）和CFP-10（culture filtrate protein-10）刺激后表達(dá)白細(xì)胞介素-22（Interleukin-22，IL-22）的特點(diǎn)，探索結(jié)核性和惡性胸水的鑒別診斷新方法。 方法：采用e-Bioscience的IL-22和干擾素-γ（Interferon-γ，IFN-γ）試劑盒和流式細(xì)胞術(shù)微球陣列法，對(duì)52例結(jié)核性胸水和35例惡性胸水中經(jīng)結(jié)核分枝桿菌特異性抗原肽刺激后PFMCs所表達(dá)的IL-22和IFN-γ濃度進(jìn)行測(cè)定，并作統(tǒng)計(jì)學(xué)分析。 結(jié)果:經(jīng)結(jié)核桿菌特異抗原肽刺激，，結(jié)核性胸水上清中PFMC的IL-22表達(dá)水平顯著升高（P＜0.05），且高于惡性胸水（P＜0.05）。結(jié)核性胸膜炎的結(jié)核桿菌特異性IL-22在胸液上清PFMC中的表達(dá)水平與IFN-γ顯著相關(guān)(相關(guān)系數(shù)r=0.3485，P=0.0320)。ROC曲線顯示，結(jié)核桿菌特異性IL-22用于結(jié)核性胸膜炎的診斷敏感性、特異性和準(zhǔn)確性分別為90.38%、80.56%和85.23%，其診斷效能與IFN-γ相當(dāng)(P＞0.05)。 結(jié)論：結(jié)核分枝桿菌抗原特異性IL-22可作為鑒別診斷胸腔積液的新指標(biāo)。
[Abstract]:Objective : To investigate the characteristics of interleukin - 22 ( IL - 22 ) after stimulation of Mycobacterium tuberculosis specific antigen peptide ( ESAT - 6 ) and P - 10 ( culture filtrate protein - 10 ) by analyzing tuberculous and malignant pleural fluid mononuclear cells ( PFs ) , and to explore new methods for differential diagnosis of tuberculous and malignant pleural effusion . Methods : The concentrations of IL - 22 and IFN - 緯 in 52 cases of tuberculous pleural effusion and 35 cases of malignant pleural effusion were measured with e - bioscience IL - 22 and interferon - 緯 ( interferon - 緯 , IFN - 緯 ) kit and flow cytometry . Results : The level of IL - 22 expression of PFMC was significantly increased ( P & lt ; 0.05 ) in the tuberculous pleural fluid by Mycobacterium tuberculosis specific antigen peptide stimulation ( P & lt ; 0.05 ) , and the expression level of TB - specific IL - 22 in pleural fluid supernatant was significantly correlated with IFN - 緯 ( r = 0.3485 , P = 0.0320 ) . ROC curves showed that the diagnostic sensitivity , specificity and accuracy of mycobacterium tuberculosis specific IL - 22 were 90.38 % , 80.56 % and 85.23 % , respectively , and their diagnostic efficacy was comparable with that of IFN - 緯 ( P > 0.05 ) . Conclusion : Mycobacterium tuberculosis antigen - specific IL - 22 can be used as a new index for differential diagnosis of pleural effusion .

【學(xué)位授予單位】：中國(guó)人民解放軍醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R521.7

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