發(fā)布時間：2019-05-11 14:45

【摘要】：目的探討含KH RNA結(jié)合結(jié)構(gòu)域信號轉(zhuǎn)導(dǎo)相關(guān)分子3(KHDRBS3)基因?qū)w外培養(yǎng)的人卵巢癌CAOV-3細胞增殖的影響。方法取對數(shù)生長期細胞,分為未轉(zhuǎn)染組、無關(guān)小干涉RNA(siRNA)序列轉(zhuǎn)染組、KHDRBS3-siRNA轉(zhuǎn)染組。采用反轉(zhuǎn)錄PCR及Western blot法驗證KHDRBS3基因的沉默效果;采用四甲基偶氮唑藍(MTT)比色法比較不同組別CAOV-3細胞增殖情況;采用流式細胞術(shù)檢測不同處理組CAOV-3細胞的細胞周期分布。結(jié)果 KHDRBS3-siRNA轉(zhuǎn)染后可明顯降低CAOV-3細胞KHDRBS3的mRNA和蛋白水平,敲低CAOV-3細胞KHDRBS3水平后,細胞的增殖能力明顯降低,G0/G1期細胞比例增加、S期細胞比例降低、G2/M期細胞比例無明顯變化。結(jié)論敲低KHDRBS3的水平可使CAOV-3細胞發(fā)生G0/G1期阻滯,抑制細胞增殖。
[Abstract]:Objective to investigate the effect of signal transduction molecule 3 (KHDRBS3) gene containing KH RNA binding domain on the proliferation of human ovarian cancer CAOV-3 cells in vitro. Methods logarithmic cells were divided into three groups: untransfected group, unrelated small interference RNA (siRNA) sequence transfer group and KHDRBS3-siRNA transfer group. The silencing effect of KHDRBS3 gene was verified by reverse transcription PCR and Western blot, and the proliferation of CAOV-3 cells in different groups was compared by (MTT) colorimetric assay. The cell cycle distribution of CAOV-3 cells in different treatment groups was detected by flow cytometry. Results after KHDRBS3-siRNA transfection, the mRNA and protein levels of KHDRBS3 in CAOV-3 cells were significantly decreased. After knocking down the KHDRBS3 level of CAOV-3 cells, the proliferation ability of CAOV-3 cells was significantly decreased, the proportion of cells in G0/G1 phase was increased, and the proportion of cells in S phase was decreased. There was no significant change in the proportion of cells in G _ 2 鈮，

本文編號：2474580