【摘要】：目的觀察中藥苦參堿(Matrine)對宮頸癌SiHa細胞增殖的抑制作用以及對JAK-STAT (Janus Kinase/Signal Transducers and Actiators of Transcription)信號轉(zhuǎn)導通路的影響。 方法1體外培養(yǎng)宮頸癌SiHa細胞,觀察細胞的生長狀態(tài),取處于對數(shù)生長期的細胞用于試驗。2試驗分組：(1)實驗組：用不同濃度的苦參堿處理宮頸癌SiHa細胞,使其終濃度分別為0.25mg/ml、0.5mg/ml、1.0mg/ml、2.0mg/ml、4.0mg/ml;(2)對照組：只加入細胞懸液,未經(jīng)藥物處理；(3)空白對照組：不接種細胞,僅加入細胞培養(yǎng)液。3應用MTT法檢測不同濃度苦參堿(0.25mg/ml、0.5mg/ml、1.0mg/ml、2.0mg/ml、4.0mg/ml)作用于宮頸癌SiHa細胞24h、48h及72h后細胞的增殖情況。4采用蛋白印跡法(Western blotting)法檢測不同濃度苦參堿(0.5mg/ml、1.0mg/ml、2.0mg/ml)作用于宮頸癌SiHa細胞48h后,SiHa細胞中JAK1、JAK2、STAT3蛋白的表達水平。 結果1苦參堿能夠抑制體外培養(yǎng)的宮頸癌SiHa細胞的增殖,該抑制作用呈時間-劑量依賴性。各組不同作用時間比較,苦參堿對宮頸癌SiHa細胞增殖的抑制率差異顯著(P0.05、0.01)。當苦參堿的加入濃度在0.25mg/ml～4.0mg/ml的范圍時,苦參堿的濃度越高,其對宮頸癌SiHa細胞的增殖抑制效應越明顯；2不同濃度的苦參堿(0.5mg/ml、1.0mg/ml、2.0mg/ml)作用于宮頸癌SiHa細胞48h后,各實驗組中JAK1、JAK2和STAT3蛋白的表達水平降低(P0.01),且有劑量依賴性。 結論苦參堿對宮頸癌SiHa細胞有抑制作用。其作用機制可能是通過降低宮頸癌細胞中JAK-STAT信號轉(zhuǎn)導通路的活性,從而抑制宮頸癌細胞的增殖,有望成為治療宮頸癌以及抑制其侵襲轉(zhuǎn)移的一種新的策略。
[Abstract]:Aim to observe the inhibitory effect of matrine (Matrine) on the proliferation of cervical cancer SiHa cells and the effect of JAK-STAT (Janus Kinase/Signal Transducers and Actiators of Transcription) signal transduction pathway). Methods (1) Cervical carcinoma SiHa cells were cultured in vitro, the growth state of the cells was observed, and the cells in logarithmic growth phase were used in the experiment. 2 the experimental groups were as follows: (1) the experimental group: the cervical cancer SiHa cells were treated with different concentrations of matrine. The final concentration is 0.25 mg / ml, 0.5 mg / ml, 1.0 mg / ml, 2.0 mg / ml, 4.0 mg / ml, respectively; (2) Control group: only cell suspension was added without drug treatment; (3) blank control group: no cells were inoculated, only cell culture medium was added. (3) different concentrations of matrine (0.25 mg / ml, 0.5 mg / ml, 1.0 mg / ml, 2.0 mg / ml) were detected by MTT method. (4) the proliferation of cervical cancer SiHa cells was detected by Western blot (Western blotting) assay after 24 h and 72 h treatment with 4.0mg/ml (0.5 mg 路ml ~ (-1) 路ml ~ (-1) ~ (-1) mg 路ml ~ (-1) of matrine (0.5 mg 路ml ~ (-1) 路ml ~ (-1), respectively. The expression of JAK1,JAK2,STAT3 protein in SiHa cells of cervical cancer treated with 2.0mg/ml for 48 h. Results 1 Matrine could inhibit the proliferation of cervical cancer SiHa cells in a time-dose-dependent manner. The inhibitory rate of matrine on the proliferation of cervical cancer SiHa cells was significantly different (P0.05 / 0. 01). When the concentration of matrine was in the range of 0.25mg/ml~4.0mg/ml, the higher the concentration of matrine was, the more obvious the inhibitory effect of matrine on the proliferation of cervical cancer SiHa cells was. 2 different concentrations of matrine (0.5 mg / ml, 1.0 mg / ml, 2.0 mg / ml) were treated with cervical cancer SiHa cells for 48 h, the expression levels of JAK1,JAK2 and STAT3 protein in each experimental group were decreased (P0.01) in a dose-dependent manner. Conclusion Matrine can inhibit SiHa cells of cervical cancer. Its mechanism may be to inhibit the proliferation of cervical cancer cells by reducing the activity of JAK-STAT signal transduction pathway in cervical cancer cells, which may become a new strategy for the treatment of cervical cancer and its invasion and metastasis.
【學位授予單位】：蘭州大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R737.33

【參考文獻】

相關期刊論文 前10條

1 伍嚴安,高春芳,王皓,黃超,孔憲濤;苦參堿抑制血小板衍生生長因子誘導的小鼠皮膚成纖維細胞增殖[J];第二軍醫(yī)大學學報;2000年08期

2 戴碧濤,蔣紀愷,劉瑜,王莉佳,王世一;苦參堿對白血病細胞分泌IL-6和TNF-α的影響[J];第三軍醫(yī)大學學報;2003年22期

3 高健;梁耀軍;金玉;李玉梅;李宇寧;;苦參堿對阿霉素誘導的腎小球硬化大鼠STAT3信號分子的影響[J];第四軍醫(yī)大學學報;2008年08期

4 申曉東,宋關斌,嚴潤彬,楊揚;苦參堿和氧化苦參堿抗腫瘤作用的研究進展[J];重慶大學學報(自然科學版);2005年06期

5 鄭志芳;朱世澤;;真核翻譯起始因子4E[J];醫(yī)學分子生物學雜志;2008年02期

6 徐寧志,董志偉;腫瘤的分子生物學基礎及其應用[J];國外醫(yī)學(腫瘤學分冊);2003年01期

7 楊茜;王英;楊愛宏;楊永秀;;苦參堿與其聯(lián)合順鉑對U14荷瘤小鼠抑制作用的研究[J];中國醫(yī)藥科學;2012年07期

8 唐雅娟;韓萍;吳維光;孫兆翎;何艷舫;辛德梅;陳昭;;STAT3基因反義寡核苷酸對人宮頸癌HeLa細胞增殖和凋亡的影響[J];蚌埠醫(yī)學院學報;2013年03期

9 文顯梅;魏蕾;張京偉;彭小春;王婷婷;張利軍;李華;;苦參堿對HeLa細胞粘附和移動的抑制作用及機制[J];武漢大學學報(醫(yī)學版);2006年02期

10 趙軍方;謝偉紅;李新明;陳萬濤;孫明磊;方政;孫強;;苦參堿對腺樣囊性癌細胞周期阻滯和端粒酶催化亞基表達的影響[J];華西口腔醫(yī)學雜志;2010年03期

，

本文編號：2352471