【摘要】：目的直接進行脂肪間充質干細胞(ADSCs)移植治療疾病,有可能因為干細胞的無限增殖能力導致治療的風險性升高,尤其是有致瘤的風險。本實驗將γ射線輻照后的ADSCs 移植入小鼠薄型子宮內膜模型,以提高治療安全性。方法 ADSCs取材于雌性SD大鼠,應用流式細胞技術進行鑒定后,分別以0、5 和10 Gy 的γ射線輻照ADSCs。24 只薄型子宮內膜模型SD大鼠隨機分為4 組,在造模6-8 h 后子宮內移植未經(jīng)輻照的ADSCs(組Ⅰ)、經(jīng)5 Gy輻照的ADSCs(組Ⅱ)、經(jīng)10 Gy輻照的ADSCs(組Ⅲ),或僅子宮內注射PBS(組Ⅳ)。測量各組大鼠細胞移植后子宮內膜的厚度變化以觀察治療效果。結果 ADSCs經(jīng)10 Gy的輻照后完全失去了增殖能力。組Ⅰ和組Ⅱ大鼠在細胞移植治療后子宮內膜厚度比組Ⅳ有顯著增加(P0.01),但是組Ⅲ和組Ⅳ大鼠的子宮內膜厚度無顯著差異。結論γ射線破壞了ADSCs的增殖能力,經(jīng)10 Gy照射后,ADSCs完全失去了增殖能力并失去了治療能力。經(jīng)5 Gy照射后的ADSCs部分喪失了增殖能力,但仍有治療效果,能夠改善子宮內膜的厚度。
[Abstract]:Objective to treat diseases by direct transplantation of adipose mesenchymal stem cells (ADSCs), it is possible that the unlimited proliferation of stem cells may lead to increased risk of treatment, especially the risk of tumorigenesis. In this study, 緯-ray irradiated ADSCs was transplanted into mouse model of thin endometrium to improve the safety of treatment. Methods ADSCs was obtained from female SD rats and identified by flow cytometry. The ADSCs.24 model SD rats were randomly divided into 4 groups, which were irradiated with 緯 -rays of 0 ~ 5 and 10 Gy for 5 Gy and 10 Gy, respectively. ADSCs (group 鈪，

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