【摘要】：目的研究生長激素受體(GHR)、雌激素受體(ER)、孕激素受體(PR)在宮腔粘連(研究組)和非宮腔粘連(對照組)患者子宮內膜組織中的表達情況,為宮腔粘連患者在經陰道宮腔鏡下宮腔粘連分離術(TCRA)術后使用生長激素、雌激素、孕激素預防再粘連提供一定的理論參考。方法1、資料來源研究組:2013年11月至2014年05月在我院婦科行宮腔鏡檢查并確診為宮腔粘連患者28例為研究組,年齡20~35歲,平均年齡29.62±2.52歲。對照組:同期因其他原因(不孕、子宮縱膈或節(jié)育器嵌頓)在宮腔鏡檢查下確診為非宮腔粘連患者22例為對照組,年齡21~40歲,平均年齡26.80±3.34歲。并且這些非宮腔粘連患者既往都有宮腔操作史。2、實驗方法2.1標本采集。研究組:患者均行宮腔鏡檢查確診為宮腔粘連,在宮腔鏡檢查中或經陰道宮腔鏡下宮腔粘連分離術操作過程中,視需要在尚殘存的正常子宮內膜組織中,用異物鉗鉗取適量(質量約20 mg)的內膜組織作為標本。對照組:患者因其他原因行宮腔鏡檢查確認為非宮腔粘連且既往都有過宮腔操作史,如子宮縱膈、不孕或節(jié)育器嵌頓,按需要鉗取適量正常子宮內膜組織。2.2實驗方法。采用免疫組化Max Vision兩步法和實時熒光定量PCR(q RTPCR)方法分別檢測兩組患者子宮內膜組織中GHR蛋白、ER蛋白、PR蛋白和GHRm RNA、ERm RNA、PR m RNA的表達情況。結果1、免疫組化Max Vision兩步法檢測研究組和對照組ER蛋白、PR蛋白、GHR蛋白表達;實時熒光定量PCR實驗方法檢測研究組和對照組ERm RNA、PRm RNA、GHRm RNA表達;兩種實驗方法檢測GHR、ER在兩組中表達有顯著統(tǒng)計學意義(P0.05),PR在兩組中表達無統(tǒng)計學意義(P0.05)。2.采用免疫組化Max Vision兩步法檢測子宮內膜腺體與子宮內膜間質中ER、PR和GHR蛋白表達,兩部位比較差異有統(tǒng)計學意義(P0.05)。結論1.研究組患者子宮內膜組織中的GHR表達低于對照組,而且這種表達在兩組子宮內膜腺體中較多,而在兩組子宮內膜間質中表達較少,為宮腔粘連患者在TCRA術后使用生長激素預防再粘連提供一定的理論參考。2.研究組患者子宮內膜組織中ER表達高于對照組,PR表達在兩組之間無差異;ER、PR在兩組子宮內膜腺體中表達較多,而在兩組子宮內膜間質中表達較少甚至不表達;為宮腔粘連患者在TCRA術后使用雌激素和孕激素預防再粘連提供一定的理論參考。
[Abstract]:Objective to study the expression of growth hormone receptor (GHR), estrogen receptor (ER), progesterone receptor (PR) in endometrium of patients with intrauterine adhesions (study group) and non-uterine adhesions (control group). To provide a theoretical reference for the prevention of adhesions by growth hormone, estrogen and progesterone in patients with intrauterine adhesions after (TCRA) under transvaginal hysteroscopy. Methods 1. Data source study group: from November 2013 to May 2014, 28 patients with intrauterine adhesion were diagnosed by hysteroscopy in our hospital. The age was 2035 years old with an average age of 29.62 鹵2.52 years. Control group: 22 cases of non-uterine adhesions diagnosed by hysteroscopy for other reasons (infertility, uterine mediastinal or IUD incarceration) were the control group. The average age was 26.80 鹵3.34 years. And these patients with non-uterine adhesions had a history of intrauterine operations of. 2. 2 and 2. 1 specimens were collected. Study group: all the patients underwent hysteroscopy to diagnose intrauterine adhesions, and during hysteroscopy or vaginal hysteroscopy, in the normal endometrial tissues which remained as needed during the operation of hysteroscopy or vaginal hysteroscopy, The appropriate amount of intimal tissue (about 20 mg mass) was obtained by using foreign body forceps. Control group: the patients were confirmed by hysteroscopy as non-intrauterine adhesions for other reasons and had previous history of uterine cavity operation, such as mediastinal uterus, infertility or incarceration of sterility or device, and appropriate amount of normal endometrial tissue .2.2 was obtained according to the need of forceps. The expression of GHR protein ER protein, PR protein and GHRm RNA,ERm RNA,PR m RNA in endometrium of two groups were detected by immunohistochemical Max Vision two-step method and real-time fluorescence quantitative PCR (q RTPCR) method. Results 1. The expression of ER protein and PR protein in study group and control group were detected by immunohistochemical Max Vision two-step method, and the expression of ERm RNA,PRm RNA,GHRm RNA in study group and control group was detected by real-time fluorescence quantitative PCR assay. Two experimental methods to detect the expression of GHR,ER in the two groups had significant statistical significance (P0.05) the expression of PR in the two groups had no statistical significance (P0.05). 2. The expression of ER,PR and GHR in endometrium and endometrial stroma was detected by immunohistochemical Max Vision two-step method. The difference between the two sites was statistically significant (P0.05). Conclusion 1. The expression of GHR in endometrial tissue of study group was lower than that in control group, and the expression was more in endometrial gland and less in stroma of endometrium in both groups. To provide a theoretical reference for the patients with intrauterine adhesion after TCRA to use growth hormone to prevent re-adhesion. 2. The expression of ER in endometrial tissue of study group was higher than that of control group. There was no difference between the two groups in the expression of ERP in the endometrial gland of the two groups, but there was little or no expression in the stroma of endometrium in the two groups. To provide a theoretical reference for the use of estrogen and progesterone in patients with intrauterine adhesion after TCRA.
【學位授予單位】：安徽醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R711.74

【共引文獻】

相關期刊論文 前10條

1 王才智;何玉;申慶文;晉茂生;;腹腔鏡輔助子宮肌瘤剔除術的臨床應用研究[J];蚌埠醫(yī)學院學報;2010年09期

2 沙吉達.賽買提;;應用宮腔鏡診斷子宮內膜息肉48例分析[J];地方病通報;2006年05期

3 胡逸君;;腹腔鏡診治婦科急腹癥98例[J];臨床醫(yī)學;2008年05期

4 孫南丁;沈進;陳麗玉;董生偉;;宮腔鏡診治宮內節(jié)育器取出困難[J];福建醫(yī)科大學學報;2007年04期

5 朱莉華;曹云桂;吳智華;;腹腔鏡手術治療宮角妊娠18例臨床分析[J];中國婦產科臨床雜志;2011年02期

6 謝曄玲;;腹腔鏡診治婦科急癥79例臨床分析[J];腹腔鏡外科雜志;2006年06期

7 趙宏輝;劉艷庚;;腹腔鏡巨大子宮肌瘤切除術的臨床體會[J];腹腔鏡外科雜志;2008年04期

8 唐艷芬;王丹霞;;腹腔鏡巨大子宮切除術78例體會[J];腹腔鏡外科雜志;2009年04期

9 李麗;孫美琴;孫棟燕;;289例婦科腹腔鏡手術的臨床分析[J];腹腔鏡外科雜志;2012年04期

10 陳作珍;左月英;;腹腔鏡下輸卵管妊娠開窗取胚術42例分析[J];華夏醫(yī)學;2008年06期

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1 王小紅;;腹腔鏡下子宮肌瘤剔除術與開腹手術的臨床療效比較[A];中國優(yōu)生優(yōu)育協(xié)會第四屆全國學術論文報告會暨基因科學高峰論壇論文專輯[C];2008年

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1 郭菲菲;60歲以上老年婦女婦科腹腔鏡手術臨床應用[D];大連醫(yī)科大學;2011年

2 孫紅燕;輸卵管遠端阻塞腹腔鏡再通術后中醫(yī)外治療法的臨床研究[D];廣州中醫(yī)藥大學;2005年

3 開麗比努爾;243例宮頸病變陰道鏡診斷的臨床分析[D];新疆醫(yī)科大學;2007年

4 王靜;腹腔鏡在輸卵管妊娠診治中的應用價值[D];新疆醫(yī)科大學;2008年

5 任麗娜;臍孔及臍上5cm處與腹膜后大血管投影的相對位置關系探討[D];第二軍醫(yī)大學;2008年

6 習春風;明膠海綿栓塞子宮肌瘤對卵巢功能的影響及與PVA療效對比[D];吉林大學;2009年

7 何偉紅;腹腔鏡下不同術式治療異位妊娠的臨床療效及預后比較[D];新疆醫(yī)科大學;2009年

8 邱中娟;自制Roeder氏壓脈帶在腹腔鏡子宮肌瘤切除術中的應用探討[D];山東大學;2010年

9 趙海鵬;異位妊娠急診診斷和治療的臨床研究[D];吉林大學;2010年

10 徐晨;婦科腹腔鏡手術中不同CO_2氣腹壓力對機體應激的影響[D];中國醫(yī)科大學;2010年

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