【摘要】：目的：探討腫瘤壞死因子α基因-857C/T、-863A/C和-1031T/C三個位點單核苷酸多態(tài)性與妊娠期糖尿病的關(guān)系以及子代（胎兒）與母親之間的關(guān)系。 方法：隨機選取2012年3月至2013年2月在北京航天總醫(yī)院婦產(chǎn)科正規(guī)產(chǎn)檢的足月妊娠孕婦100例，將其分為妊娠期糖尿病組（GDM組）和正常孕婦（對照組）組，每組各50例，抽取孕婦外周靜脈血及其新生兒臍帶血，提取DNA，采用PCR限制性酶切片段長度分析-857C/T、-863A/C和-1031T/C，測序驗證。應(yīng)用2檢驗比較基因型分布是否符合Hardy-Weinberg平衡。結(jié)果采用t檢驗和2檢驗等統(tǒng)計學(xué)分析。 結(jié)果：TNF-α基因-857C/T位點的基因型分布在GDM組與正常孕婦組有顯著差異，，其中-857位點在GDM組中CT基因型及T等位基因頻率所占比例高于正常孕婦組， P0.05；另外，在基因型為CC的母親中，GDM組胎兒CT型比例高于正常對照組胎兒， P0.05。而-1031C/T和-863A/C位點無論是基因型還是等位基因頻率在兩組母親中均無差異，P0.05。三個位點的基因型分布在其胎兒中均有顯著差異，其中-857C/T位點在GDM組胎兒中CT基因型及T等位基因頻率所占比例高于正常孕婦組胎兒，P0.05。-863A/C位點在GDM組胎兒中CA基因型及A等位基因頻率所占比例高于正常孕婦組胎兒， P 0.05。-1031T/C位點在GDM組胎兒中CT基因型及C等位基因頻率所占比例高于正常孕婦組胎兒,P0.05。 結(jié)論：1.TNF-α基因-857C/T等位基因多態(tài)性與GDM的發(fā)病有關(guān)；-863A/C和-1031C/T兩個位點可能與GDM無關(guān)。 2.胎兒的TNF-α基因3種SNP可增加母親患GDM風(fēng)險。
[Abstract]:Aim: to investigate the relationship between the single nucleotide polymorphisms (SNP) at three loci of tumor necrosis factor 偽 gene -857C- 863A / C and -1031T / C and gestational diabetes mellitus (GDM), as well as the relationship between the offspring (fetus) and the mother. Methods: from March 2012 to February 2013, 100 pregnant women in Beijing Aerospace General Hospital were randomly divided into gestational diabetes mellitus (GDM) group and normal pregnant women (control group) group, with 50 pregnant women in each group. Peripheral venous blood of pregnant women and umbilical cord blood of newborns were extracted. PCR restriction fragment length analysis was used to analyze -857C / T- 863A / C and -1031T / C respectively. The genotype distribution was compared with Hardy-Weinberg equilibrium by test 2. Results t test and 2 test were used for statistical analysis. Results there was significant difference in the genotype distribution of -857C / T locus between GDM group and normal pregnant woman group, and the proportion of CT genotype and T allele frequency in GDM group was higher than that in normal pregnant woman group (P0.05). The proportion of fetal CT type in GDM group was higher than that in normal control group (P0.05). The frequencies of genotype and allele at -1031 C / T and -863 A / C loci were not significantly different between the two groups (P 0.05). The genotypes of the three loci were significantly different in their fetuses. The frequency of CT genotype and T allele in GDM group was higher than that in normal pregnant woman group (P 0.05.-863 A / C locus). CA genotype and A allele frequency in GDM group was higher than that in normal pregnant woman group. The percentage of CT genotype and C allele frequency in GDM group was higher than that in normal pregnant women group (P 0.05). Conclusion: 1. TNF- 偽 gene -857C / T allele polymorphism is associated with the pathogenesis of GDM. The two loci of -863 A / C and -1031 C / T may not be associated with GDM. 2. Fetal TNF- 偽 gene three types of SNP may increase the maternal risk of GDM.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R714.256

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