【摘要】：目的：探討葉酸對高血糖孕鼠子代心臟發(fā)育相關蛋白GATA4、Nkx2.5表達的影響。方法：將受孕的SD大鼠40只隨機均分為5組：正常對照組、模型組、葉酸第3天組（FA3rd組）、葉酸第7天組（FA7th組）、葉酸第15天組（FA15th組）；確認受孕后，除正常對照組外，其他4個組一次性按38mg/kg予腹腔注射鏈脲佐菌素（streptozotocin, STZ）復制高血糖孕鼠模型，，其中FA3rd組、FA7th組、FA15th組3個干預組分別從孕第3、7、15天開始，每天予葉酸0.8mg/kg灌胃至妊娠結束。在孕鼠孕21d時取子鼠心臟，HE染色觀察子鼠心��；免疫組化（Immunohistochemistry）及蛋白免疫印跡（Western blot）檢測子鼠心臟發(fā)育相關基因GATA4、Nkx2.5的蛋白表達。結果：STZ干預后孕鼠血糖明顯升高（P0.05），部分子鼠心肌可見損傷；免疫組化顯示，GATA4蛋白在心房、心室、室間隔、瓣膜均有表達；Nkx2.5蛋白在心房、心室、室間隔均有表達，但在瓣膜未見明顯表達；Western blot顯示，與正常對照組比較，STZ干預組GATA4及Nkx2.5蛋白表達均下調（P0.05），F(xiàn)A3rd組及FA7th組的蛋白表達水平均顯著高于模型組（P0.05），且FA3rd組的蛋白上調程度高于FA7th組（P0.05），F(xiàn)A15th組與模型組的蛋白表達水平差異無統(tǒng)計學意義。結論：孕鼠的高血糖狀態(tài)可使子代心臟損傷及心臟發(fā)育相關蛋白GATA4、Nkx2.5表達水平下調；及時補充葉酸可上調GATA4、Nkx2.5蛋白表達水平，對處于母體高血糖狀態(tài)影響下的子鼠心臟的發(fā)育可能具有保護作用。
[Abstract]:Aim: to investigate the effect of folic acid on the expression of cardiac development-associated protein GATA4 and Nkx2.5 in the offspring of hyperglycemic pregnant rats. Methods: forty pregnant SD rats were randomly divided into 5 groups: normal control group, model group, folic acid 3rd day group (FA3rd group), folic acid 7th day group (FA7th group), folic acid 15th day group (FA15th group). The other four groups were injected intraperitoneally with streptozotocin (38mg/kg) to induce hyperglycemia in pregnant rats. The rats in FA3rd group were given folic acid 0.8mg/kg daily until the end of pregnancy, respectively, from the 3rd day of gestation to the end of pregnancy in three intervention groups (FA3rd group, FA7 th group and FA15 th group). At the 21st day of gestation, the neonatal rat heart was stained with HE, and the expression of GATA4Nkx2.5 was detected by immunohistochemical (Immunohistochemistry) and Western blot (Western blot). Results the blood glucose of pregnant rats increased significantly (P0.05), and the myocardial damage was observed in some of the offspring. Immunohistochemical staining showed that GATA4 protein was expressed in atrial, ventricular, ventricular septum and valvular septum, and Nkx2.5 protein was expressed in atrium, ventricle and interventricular septum. However, there was no obvious expression of Western blot in valvular valve. Compared with the normal control group, the protein expression of GATA4 and Nkx2.5 in the STZ-treated group was significantly lower than that in the model group (P0.05). The protein expression level in the FA3rd group was significantly higher than that in the model group (P0.05), and the protein expression level in the FA3rd group was higher than that in the FA7th group (P0.05) and the model group (P0.05). The difference was not statistically significant. Conclusion: the hyperglycemia of pregnant rats can down-regulate the expression of GATA4Nkx2.5, and timely supplementation of folic acid can up-regulate the expression of GATA4Nkx2.5 protein. It may have protective effect on the heart development of the offspring under the influence of maternal hyperglycemia.
【學位授予單位】：桂林醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R714.256

【參考文獻】

相關期刊論文 前8條

1 劉俊;王燕舞;章宏峰;孟運蓮;;葡萄糖對胚胎發(fā)育的致畸作用研究[J];中國組織化學與細胞化學雜志;2009年04期

2 盧艷;王海琴;王新;;高同型半胱氨酸血癥孕鼠與其仔鼠發(fā)生先天性心臟病的關系[J];中南大學學報(醫(yī)學版);2011年01期

3 江燕;石珍;李敏;;葉酸對高血糖孕鼠胎鼠心肌細胞凋亡的影響[J];南昌大學學報(醫(yī)學版);2010年02期

4 趙娜;李素梅;;妊娠期糖尿病發(fā)病機制的研究進展[J];國際病理科學與臨床雜志;2012年02期

5 李衛(wèi)紅;肖強;李善英;商戰(zhàn)平;吳同果;;葉酸對2型糖尿病大鼠心肌細胞凋亡的影響[J];中國臨床康復;2006年44期

6 仇小強;鐘秋安;曾小云;沈立萍;聶紹發(fā);;孕期環(huán)境暴露因素與先天性心臟病關系[J];中國公共衛(wèi)生;2008年05期

7 李勇,李松,陳光慧,胡白和,孟昭亨,陳星,趙如冰,張晨暉,劉虹,趙智榕,湯健,李竹;HCY-2基因和同型半胱氨酸與雞胚先天性心臟畸形發(fā)生的關系[J];中華醫(yī)學雜志;2000年02期

8 劉偉;陶芳標;汪思應;黃德武;王曉紅;;NKx2.5基因在孕鼠肥胖致胚胎心臟畸形中的表達及意義[J];安徽醫(yī)科大學學報;2013年01期

相關博士學位論文 前1條

1 梁進濤;高糖對斑馬魚胚胎心臟發(fā)育的影響及機制研究[D];復旦大學;2010年

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