本文選題：子宮內膜異位癥 + 遷移能力　； 參考：《中南大學》2014年碩士論文

【摘要】：目的 本課題通過原代培養(yǎng)正常人及子宮內膜異位癥患者子宮內膜細胞,比較不同細胞的形態(tài)學結構及遷移能力的差別。通過給予不同細胞雌、孕激素及原礬酸鈉(SoV)干預,測定肝再生磷酸酶3(phosphatase of regenerating liver-3, PRL-3)的表達情況及變化,探討PRL-3與子宮內膜細胞遷徙能力差異的關系,研究子宮內膜異位癥高轉移高侵襲的發(fā)病機制。 方法 1.原代培養(yǎng)正常人及子宮內膜異位癥患者子宮內膜細胞,比較不同細胞的形態(tài)學結構。 2.用劃線實驗比較不同細胞的遷移能力差別。 3. qT-PCR法及Western Blot法測定不同細胞中PRL-3的表達。 4.予以PRL-3抑制劑原礬酸鈉、雌、孕激素干預后,行劃線實驗確定細胞遷移能力變化。qT-PCR法及Western Blot法測定不同細胞中PRL-3的表達。 結果 1.子宮內膜細胞由腺上皮細胞及間質細胞組成,腺上皮細胞角蛋白免疫組化染色呈陽性反應,間質細胞波形蛋白免疫組化染色呈陽性反應。隨著傳代次數(shù)增加,角蛋白陽性細胞數(shù)量越來越少,波形蛋白陽性細胞所占比例占絕大部分,為主要細胞。 2.劃線實驗結果顯示：在位內膜細胞在36小時后細胞遷移的距離大于正常子宮內膜細胞遷移的距離；對照組內膜細胞遷移的距離大于加入0.5umol/l SoV組細胞遷移的距離；10-6mol/l雌二醇組細胞遷移的距離大于對照組細胞遷移的距離。對照組內膜細胞遷移的距離大于10-5mol/l孕酮細胞遷移的距離。 3.PRL-3在EMs患者在位內膜上皮細胞中表達強于正常人細胞中的表達,兩者之間差異有統(tǒng)計學意義(P0.05)；對照組細胞中PRL-3表達強于加入SoV組細胞中表達,兩者之間差異有統(tǒng)計學意義(P0.05)；加入雌激素組細胞中PRL-3表達強于對照組細胞中表達,兩者之間差異有統(tǒng)計學意義(P0.05)；對照組細胞中PRL-3表達強于加入孕激素組細胞中的表達,兩者之間差異有統(tǒng)計學意義(P0.05) 結論 1.EMs患者在位子宮內膜細胞遷移能力強于正常人子宮內膜細胞遷移能力,PRL-3在EMs在位子宮內膜細胞中表達強于正常人子宮內膜細胞,子宮內膜細胞可能通過PRL-3促進子宮內膜細胞的遷移能力。 2.雌激素可能通過上調PRL-3表達,正性調節(jié)子宮內膜細胞的遷移。孕激素可能通過下調PRL-3表達,負性調節(jié)子宮內膜細胞的遷移。 3.SoV能通過抑制PRL-3的表達,抑制子宮內膜細胞的遷移能力。
[Abstract]:Purpose

In order to study the relationship between PRL - 3 and migration ability of endometrial cells , the relationship between PRL - 3 and migration ability of endometrial cells was determined by the intervention of estrogen , progesterone and sodium sulfate ( SoV ) in different cells .

method

1 . The endometrial cells of normal and endometriosis were cultured in primary culture , and the morphological structure of different cells was compared .

2 . The difference of migration ability of different cells was compared by scoring experiment .

3 . The expression of PRL - 3 in different cells was determined by qT - PCR and Western Blot .

4 . The expression of PRL - 3 in different cells was determined by the method of qT - PCR and Western Blot .

Results

1 . The endometrial cells were composed of glandular epithelial cells and stroma cells . The cytokeratins of the glandular epithelial cells showed a positive reaction with immunohistochemical staining . The number of keratin - positive cells decreased with the increase of the number of passages , and the percentage of the positive cells of the protein - positive cells accounted for most of the cells , which were the main cells .

2 . The results showed that the migration distance of the cells after 36 hours was greater than that of normal endometrial cells .
The migration of endometrial cells in the control group was greater than that in the group of 0.5mol / l SoV group .
The distance of migration of cells from 10 - 6 mol / l estradiol group was greater than that of control group .

3 . The expression of PRL - 3 in the epithelial cells of endometriosis was significantly higher than that in normal human cells ( P0.05 ) .
The expression of PRL - 3 in the control group was stronger than that in control group ( P0.05 ) .
PRL - 3 expression in estrogen group was stronger than that in control group ( P0.05 ) .
The expression of PRL - 3 in the control group was stronger than that in control group ( P0.05 ) .

Conclusion

1 . In endometriosis , the migration ability of endometrium cells was stronger than that of normal controls , PRL - 3 expression was stronger than normal endometrium cells in endometriosis , and the migration ability of endometrial cells could be promoted by PRL - 3 .

2 . Estrogen may regulate the migration of endometrial cells by up - regulating the expression of PRL - 3 , and progesterone may regulate the migration of endometrial cells by downregulating PRL - 3 expression .

3 . SoV can inhibit the migration of endometrial cells by inhibiting the expression of PRL - 3 .
【學位授予單位】：中南大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R711.71

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相關期刊論文 前2條

1 張平;張志培;李香敏;雷杰;蘇凱;李小飛;王小平;;PRL-3和RhoC在A549細胞中的表達及意義[J];中國肺癌雜志;2010年12期

2 張建龍;張縈斐;張育超;毛凱;陳雙;;PRL-3調節(jié)PI3K信號通路在促進結腸癌細胞增殖侵襲中的作用[J];中山大學學報(醫(yī)學科學版);2013年01期

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