本文選題：宮頸癌 + Wnt信號通路�。� 參考：《皖南醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:檢測β-catenin、Wnt3a和Lgr5在不同宮頸組織中的表達(dá)情況,并分析三者之間表達(dá)相關(guān)性,探討三者與宮頸癌發(fā)生發(fā)展的關(guān)系。方法:采用免疫組織化學(xué)法(SP法)檢測不同宮頸組織標(biāo)本中β-catenin、Wnt3a和Lgr5的表達(dá),包括宮頸癌組織61例,宮頸上皮內(nèi)瘤變組織30例(CINI12例,CINII-III 18例),正常宮頸組織19例.結(jié)果:1.β-catenin正常情況下為連續(xù)的包膜表達(dá),病理狀態(tài)時,則出現(xiàn)胞膜表達(dá)的缺失,胞質(zhì)/核內(nèi)出現(xiàn)不同程度的異常表達(dá)。β-catenin異常表達(dá)率在正常宮頸組織組5.26%(1/19),CINI組33.33%(4/12),CINII-III組38.89%(7/18),CC組75.41%(46/61),4組比較(χ_2=32.958,P0.05),有統(tǒng)計學(xué)意義;在CC組G1、G2、G3各級的異常表達(dá)率分別為41.67%(5/12)、76.67%(23/30)、94.74%(18/19),可以看出在G3組表達(dá)突出(χ_2=11.221,P0.05)有統(tǒng)計學(xué)意義;在淺浸潤組和深浸潤組異常表達(dá)率分別為55.56%(10/18)和83.72%(36/43)(χ_2=5.428,P0.05),有統(tǒng)計學(xué)意義。而其異常表達(dá)與臨床分期、年齡、組織學(xué)類型、年齡可能無關(guān)(P0.05)。2.Wnt3a陽性表達(dá)率在正常宮頸組織組10.53%(2/19),CINI組41.67%(5/12),CINII-III組55.56%(10/18),CC組40.98%(25/61),4組比較(χ_2=8.723,P0.05),有統(tǒng)計學(xué)意義;在淋巴結(jié)轉(zhuǎn)移組為59.09%(13/22),無淋巴結(jié)轉(zhuǎn)移組30.78%(12/39),(χ_2=4.665,P0.05),有統(tǒng)計學(xué)意義。而Wnt3a在臨床分期、病理分級、浸潤深淺、組織學(xué)類型、年齡的陽性表達(dá)可能無關(guān)(P0.05)。3.Lgr5陽性表達(dá)率在正常宮頸組織組0%(0/19),CINI組25%(3/12),CINII-III組38.89%(7/18),CC組65.57%(40/61),4組比較(χ_2=28.130,P0.05),有統(tǒng)計學(xué)意義。Lgr5在宮頸癌G1 33.33%(4/12)、G2 70%(21/30)、G3 78.95%(15/19)各級的陽性表達(dá)有統(tǒng)計學(xué)意義(χ_2=7.291);在淺浸潤組和深浸潤組異常表達(dá)率分別為38.89%(7/18)和76.74%(33/43)(χ_2=6.149,P0.05),也有統(tǒng)計學(xué)意義。而Lgr5在臨床分期、病理分級、浸潤深淺、組織學(xué)類型、年齡的陽性表達(dá)可能無關(guān)(P0.05)。4.在β-catenin和Wnt3a在CC組中共陽性表達(dá)23例,共陰性表達(dá)13例,兩者之間表達(dá)呈正相關(guān)(r=0.321);β-catenin和Lgr5在CC組共陽性表達(dá)35例,共陰性表達(dá)10例,兩者之間表達(dá)呈正相關(guān)(r=0.387);Wnt3a和Lgr5在CC組共陽性表達(dá)13例,共陰性表達(dá)9例,兩者之間表達(dá)無統(tǒng)計學(xué)意義(P0.05)。結(jié)論:1.β-catenin、Wnt3a和Lgr5在正常宮頸組織、CINI、CINII-III及CC中,β-catenin的異常表達(dá)、Wnt 3a和Lgr5的陽性表達(dá)均呈逐漸上升趨勢,提示β-catenin、Wnt3a和Lgr5可能影響宮頸癌的發(fā)生發(fā)展。2.Wn3a可能與宮頸癌前病變的發(fā)生及宮頸癌的轉(zhuǎn)移有關(guān),β-catenin和Lgr5可能與宮頸癌的惡性程度及侵襲性有關(guān)3.Lgr5與β-catenin、Wnt 3a與β-catenin的表達(dá)具有一致性,呈正相關(guān),提示Lgr5與Wnt 3a的表達(dá)可能激活了wnt信號通路,導(dǎo)致了β-catenin異位至胞質(zhì)/核內(nèi),進(jìn)而引起細(xì)胞異常增生及宮頸癌的發(fā)生。
[Abstract]:Objective: to detect the expression of 尾 -cateninine Wnt3a and Lgr5 in different cervical tissues, and to analyze the relationship between the expression of Wnt3a and Lgr5, and to explore the relationship between the expression of Wnt3a and Lgr5 in the occurrence and development of cervical cancer. Methods: immunohistochemical staining was used to detect the expression of 尾 -cateninine Wnt3a and Lgr5 in different cervical tissues, including 61 cases of cervical carcinoma, 30 cases of cervical intraepithelial neoplasia, 12 cases of CINII-III and 19 cases of normal cervical tissue. Results: 1. 尾 -catenin was expressed in a continuous capsule under normal conditions, but the cell membrane expression was absent in pathological state. The abnormal expression rate of 尾 -catenin in normal cervix tissue group was 5.26 / 119% in normal cervix tissue group and 33.3333 / 12% in CINI group and 38.89% / 12% in CINII-III group (P < 0.05). In CC group, the abnormal expression rates of G1G2G3 were 41.67% and 51.67%, respectively. There were significant differences in abnormal expression in G3 group (蠂 211.221 P 0.05), in shallow infiltrating group and deep infiltrating group were 55.56% 10 / 18 and 83.722% 36 / 43 (蠂 2 / 25.428 P 0.05 P 0.05, respectively). However, the abnormal expression of CINII-III was not correlated with clinical stage, age, histological type and age. 2. The positive rate of Wnt3a expression in normal cervix tissue group was 10.53 / 20% CINI group (41.67%) and CINII-III group (55.56% / 18CC group, 40.98% 25 / 61%) group (蠂 ~ 2T = 8.723 P 0.05). In the lymph node metastasis group, it was 59.09 / 13 / 22, and in the non-lymph node metastasis group 30.78 / 39 / 39 (蠂 ~ 24.665 / P0.05), which was statistically significant. And Wnt3a in clinical stage, pathological grade, invasion depth, histological type, The positive expression of age may not be related to the positive rate of P0.05. 3. The positive expression rate of Lgr5 in normal cervix tissue group was 38.89% CINII-III and 65.5728.130% P0.05 in normal cervical tissue group (蠂 228.130P0.05). There was statistical significance at all levels (蠂 228.130P 0.05). There was statistical significance at all levels (蠂 228.130P0.05, P 0.05). There was statistical significance at all levels (蠂 228.130P0.05, P 0.05). There was statistical significance at all levels (蠂 228.130P0.05, P 0.05). There was statistical significance at all levels of G3 78.951519 in normal cervical tissue group (蠂 228.130P0.05) (蠂 228.130P 0.05). There was statistical significance at all levels (蠂 228.130P 0.05, P 0.05) in CC group (蠂 228.130P 0.05). The abnormal expression rates were 38.89 / 18 and 76.74 / 43 respectively in the deep infiltrating group (蠂 2 + 6.149% P 0.05), which were also statistically significant. However, the positive expression of Lgr5 in clinical stage, pathological grade, depth of invasion, histological type and age may not be related to P0.05. 4. The positive expression of 尾 -catenin and Wnt3a was positive in 23 cases and negative expression in 13 cases in CC group, and the positive expression of 尾 -catenin and Lgr5 was positive in 35 cases and negative expression in 10 cases in CC group, the positive expression of 尾 -catenin and Wnt3a in CC group was positively correlated with that of 尾 -catenin and Wnt3a in CC group. The positive expression of Wnt3a and Lgr5 in CC group was positive in 13 cases and negative in 9 cases. There was no significant difference in the expression of Wnt3a and Lgr5 between the two groups. Conclusion the abnormal expression of 尾 -catenin Wnt3a and Lgr5 in CINII-III and CC of normal cervix tissues showed an increasing tendency of Wnt3a and Lgr5. These results suggest that 尾 -cateninine Wnt3a and Lgr5 may affect the occurrence and development of cervical cancer. 2. Wn3a may be related to the occurrence of precancerous lesions and metastasis of cervical cancer, 尾 -catenin and Lgr5 may be related to the malignancy and invasiveness of cervical carcinoma 3.The expression of Lgr5 and 尾 -catenin WNT 3a and 尾 -catenin may be consistent. The positive correlation suggested that the expression of Lgr5 and Wnt 3a might activate the wnt signaling pathway, leading to 尾 -catenin ectopic into cytoplasm / nucleus, and then to the abnormal proliferation of cells and the occurrence of cervical cancer.
【學(xué)位授予單位】：皖南醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R737.33

【參考文獻(xiàn)】

相關(guān)期刊論文 前5條

1 江曉紅;張燕萍;;Lgr5在子宮頸鱗狀細(xì)胞癌中的表達(dá)及其與預(yù)后的相關(guān)性[J];臨床與實驗病理學(xué)雜志;2016年03期

2 Miguel Angel Chiurillo;;Role of the Wnt/β-catenin pathway in gastric cancer: An indepth literature review[J];World Journal of Experimental Medicine;2015年02期

3 劉春蘭;繆維芳;;wnt-1、wnt-3a、β-catenin在宮頸上皮內(nèi)瘤變和宮頸鱗癌中的表達(dá)及與腫瘤發(fā)病的相關(guān)性研究[J];中國病原生物學(xué)雜志;2014年07期

4 張孝艷;喬玉環(huán);王琳;郭瑞霞;;宮頸癌組織中sFRP1和β-catenin的表達(dá)及其意義[J];中國癌癥雜志;2011年08期

5 許良中，，楊文濤;免疫組織化學(xué)反應(yīng)結(jié)果的判斷標(biāo)準(zhǔn)[J];中國癌癥雜志;1996年04期

相關(guān)碩士學(xué)位論文 前4條

1 呂桂帥;LGR5通過調(diào)控Wnt/β-catenin通路促進(jìn)肝臟腫瘤的干性[D];第二軍醫(yī)大學(xué);2015年

2 邢春瑤;CDK8和β-catenin在宮頸鱗狀細(xì)胞癌中的表達(dá)及臨床意義[D];吉林大學(xué);2014年

3 鄧云;LGR5在宮頸癌組織表達(dá)的臨床意義及其對宮頸癌細(xì)胞生物學(xué)行為的影響[D];蘇州大學(xué);2014年

4 聶艷婷;β-catenin和DKK-1在宮頸鱗癌中的表達(dá)及意義[D];中南大學(xué);2010年

本文編號：2003893