本文選題：miRNA-506 + 上皮性卵巢癌細胞�。� 參考：《暨南大學》2017年碩士論文

【摘要】：目的:探討miRNA-506對上皮性卵巢癌細胞增殖及侵襲遷移能力的影響方法:1.采用qRT-PCR檢測上皮性卵巢癌細胞株A2780、ES-2、Ovar-3和SK-OV-3中miRNA-506的表達水平,篩選出相對高表達及相對低表達miRNA-506的上皮性卵巢癌細胞株。2.利用miRNA-506 inhibitor質(zhì)粒,轉染相對高表達miRNA-506的上皮性卵巢癌細胞株;利用miRNA-506 mimics質(zhì)粒轉染相對低表達miRNA-506的上皮性卵巢癌細胞株。3.采用CCK8檢測miRNA-506對上皮性卵巢癌細胞增殖的影響。4.采用Transwell小室方法檢測miRNA-506對上皮性卵巢癌細胞侵襲遷移能力的影響。結果:1.上皮性卵巢癌細胞株A2780、ES-2、Ovar-3和SK-OV-3中,細胞系A2780的miRNA-506表達水平相對最高,SK-OV-3相對最低。2.轉染miRNA-506 inhibitor質(zhì)粒的上皮性卵巢癌細胞系A2780的A450值為2.3095±0.0117,陰性轉染對照組的A450值為2.2216±0.0228,差異有統(tǒng)計學意義(P0.05);轉染miRNA-506 mimics質(zhì)粒的上皮性卵巢癌細胞系SK-OV-3的A450值為2.0106±0.0950,陰性轉染對照組的A450值為2.1744±0.0436,差異有統(tǒng)計學意義(P0.05)。3.轉染miRNA-506 inhibitor質(zhì)粒的上皮性卵巢癌細胞系A2780的遷移細胞數(shù)為126±10.91,陰性轉染對照組的遷移細胞數(shù)為95±11.11,差異有統(tǒng)計學意義(P0.05);轉染miRNA-506 mimics質(zhì)粒的上皮性卵巢癌細胞系SK-OV-3的遷移細胞數(shù)為103.2±9.78,陰性轉染對照組的遷移細胞數(shù)為121.0±5.61,差異有統(tǒng)計學意義(P0.05)。4.轉染miRNA-506 inhibitor質(zhì)粒的上皮性卵巢癌細胞系A2780的侵襲細胞數(shù)為:25.0±14.20,陰性轉染對照組的侵襲細胞數(shù)為16.6±7.89,差異無統(tǒng)計學意義(P0.05);5.轉染miRNA-506 mimics質(zhì)粒的上皮性卵巢癌細胞系SK-OV-3的細胞數(shù)為52.4±8.47,其陰性轉染對照組的侵襲細胞數(shù)為67.2±9.68,差異有統(tǒng)計學意義(P0.05)。結論:1.在上皮性卵巢癌細胞系中,抑制miRNA-506表達后,促進A2780細胞的增殖能力;過表達miRNA-506后,抑制SK-OV-3細胞的增殖能力。表明miRNA-506可抑制上皮性卵巢癌細胞的增殖能力。2.在上皮性卵巢癌細胞系中,抑制miRNA-506表達后,促進A2780細胞遷移能力;過表達miRNA-506后,抑制SK-OV-3細胞遷移能力。表明miRNA-506可抑制上皮性卵巢癌細胞的遷移能力。3.在上皮性卵巢癌細胞系中,抑制miRNA-506表達后,促進A2780細胞侵襲能力;過表達miRNA-506后,抑制SK-OV-3細胞侵襲能力。表明miRNA-506可抑制上皮性卵巢癌細胞的侵襲能力。
[Abstract]:Objective: to investigate the effect of miRNA-506 on proliferation, invasion and migration of epithelial ovarian cancer cells. The expression of miRNA-506 in epithelial ovarian cancer cell line A2780OES-2Ovar-3 and SK-OV-3 was detected by qRT-PCR, and the epithelial ovarian cancer cell line. 2 was screened out with relatively high expression and relatively low expression of miRNA-506. MiRNA-506 inhibitor plasmid was used to transfect epithelial ovarian cancer cell line with relatively high expression of miRNA-506, and miRNA-506 mimics plasmid was used to transfect epithelial ovarian cancer cell line. 3. The effect of miRNA-506 on proliferation of epithelial ovarian cancer cells was detected by CCK8. 4. 4. The effect of miRNA-506 on invasion and migration of epithelial ovarian cancer cells was detected by Transwell chamber method. The result is 1: 1. In epithelial ovarian cancer cell line A2780 / ES-2Ovar-3 and SK-OV-3, the expression of miRNA-506 in A2780 cell line was the highest and the lowest in SK-OV-3 cell line. The A450 value of epithelial ovarian cancer cell line A2780 transfected with miRNA-506 inhibitor plasmid was 2.3095 鹵0.0117, the A450 value of negative transfection control group was 2.2216 鹵0.0228, the difference was statistically significant (P 0.05), and the A450 value of epithelial ovarian cancer cell line SK-OV-3 transfected with miRNA-506 mimics plasmid was 2.0106 鹵0.0950. The A450 value of the radiation group was 2.1744 鹵0.0436, and the difference was statistically significant (P 0.05). The number of migration cells of epithelial ovarian cancer cell line A2780 transfected with miRNA-506 inhibitor plasmid was 126 鹵10.91, while that of negative control group was 95 鹵11.11.The difference was statistically significant (P0.05). The migration of epithelial ovarian cancer cell line SK-OV-3 transfected with miRNA-506 mimics plasmid was fine. The cell number was 103.2 鹵9.78, and the number of migration cells in negative transfected control group was 121.0 鹵5.61. The difference was statistically significant (P 0.05). The number of invasive cells of epithelial ovarian cancer cell line A2780 transfected with miRNA-506 inhibitor plasmid was 1: 25.0 鹵14.20, while that of negative transfection control group was 16.6 鹵7.89.The difference was not statistically significant (P 0.05). The number of SK-OV-3 cells transfected with miRNA-506 mimics plasmid was 52.4 鹵8.47, while the number of invasive cells in negative transfected control group was 67.2 鹵9.68. The difference was statistically significant (P 0.05). Conclusion 1. In epithelial ovarian cancer cell line, inhibition of miRNA-506 expression promoted the proliferation of A2780 cells, and over-expression of miRNA-506 inhibited the proliferation of SK-OV-3 cells. The results showed that miRNA-506 could inhibit the proliferation of epithelial ovarian cancer cells. In epithelial ovarian cancer cell line, inhibition of miRNA-506 expression promoted the migration of A2780 cells, and over-expression of miRNA-506 inhibited the migration of SK-OV-3 cells. It is suggested that miRNA-506 can inhibit the migration ability of epithelial ovarian cancer cells. In epithelial ovarian cancer cell line, inhibition of miRNA-506 expression promoted the invasion ability of A2780 cells, and overexpression of miRNA-506 inhibited the invasion ability of SK-OV-3 cells. These results suggest that miRNA-506 can inhibit the invasion of epithelial ovarian cancer cells.
【學位授予單位】：暨南大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R737.31

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