本文選題：子癇前期 + XIAP��； 參考：《廣西醫(yī)科大學》2014年碩士論文

【摘要】：目的：探討分析XIAP、VEGF在子癇前期組孕婦與對照組孕婦血清、胎盤組織中的表達情況，研究其對子癇前期的影響及二者的相關性，為子癇前期臨床治療及科研工作提供依據。 方法：選取30例2012年11月至2013年7月在廣西醫(yī)科大學第一附屬醫(yī)院住院孕產婦中，子癇前期的患者30例，其中20例輕度子癇前期患者，10例重度子癇前期患者。隨機抽選同期妊娠晚期的正常孕婦共20例做為對照組。采集病例的臨床特征資料進行比較；采用親和免疫細胞化學技術鏈霉素－生物素－過氧化物酶復合物技術（SABC）法測定胎盤組織中XIAP、VEGF蛋白的表達。通過ELISA技術對XIAP、VEGF在正常妊娠孕婦和子癇前期孕婦外周血中的表達含量進行測定。 結果：（1）子癇前期患者血清中XIAP、VEGF表達水平分別為（203.92士19.97）mg/L、（116.32士11.86）mg/L，對照組孕婦血清中XIAP、VEGF表達水平分別為（300.88士25.50）mg/L、（164.47士15.47）mg/L。XIAP、VEGF在子癇前期組和正常對照組血清中的表達量差異具有統計學意義(P均＜0.05)；XIAP、VEGF在輕度子癇前期組血清中的表達量分別為（215.56士9.60）mg/L、（122.81士8.24）mg/L，XIAP、VEGF在重度子癇前期組血清中的表達量分別為（180.62士13.66）mg/L、(103.32士5.35）mg/L，輕、重度子癇前期組血清中XIAP、VEGF相對表達量差異均有統計學意義，XIAP、VEGF在血清中的表達量呈正相關（r=0.929）。（2）XIAP在對照組、輕度和重度子癇前期組胎盤組織中的陽性表達率分別為100%、84%和62%，其中輕度子癇前期組與對照組相比，差異有統計學意義(χ2=17.391，P=0.000)，重度子癇前期組與輕度子癇前期組相比，差異有統計學意義(χ2=9.019，P=0.003)；VEGF在對照組、輕度和重度子癇前期組胎盤組織中的陽性表達率分別為100%、91%和72%，其中輕度患者組與對照組相比，差異有統計學意義(χ2=10.497，，P=0.001)，重度患者組與輕度患者組相比，差異有統計學意義(χ2=9.269，P=0.002)。 結論： XIAP、VEGF可能參與了子癇前期的發(fā)生發(fā)展。
[Abstract]:Objective: to investigate the expression of XIAPP VEGF in serum and placenta of pregnant women with preeclampsia and control group, and to study its effect on preeclampsia and the correlation between them, and to provide the basis for clinical treatment and scientific research of preeclampsia. Methods: from November 2012 to July 2013, 30 pregnant women with pre-eclampsia were enrolled in the first affiliated Hospital of Guangxi Medical University, including 20 patients with mild pre-eclampsia and 10 patients with severe pre-eclampsia. Twenty normal pregnant women in the third trimester of pregnancy were randomly selected as control group. The expression of VEGF protein in placenta was determined by streptomycin-biotin-peroxidase complex technique. The expression of ELISA in peripheral blood of normal pregnant women and preeclampsia pregnant women was determined by ELISA technique. Results the expression level of XIAPN VEGF in serum of preeclampsia patients was 203.92 鹵19.97 mg / L, respectively. The expression level of XIAPN VEGF in serum of pregnant women in control group was 300.88 + 25.50 mg / L, respectively. The expression of XIAPN VEGF in serum of preeclampsia group was different from that of normal control group (P < 0.05). There was a significant difference between preeclampsia group and normal control group in the expression of XIAPP VEGF in serum of preeclampsia group and normal control group. The expression of VEGF in the serum of mild preeclampsia group was 215.56 鹵9.60 mg / L 122.81 鹵8.24 mg / L respectively. The expression of VEGF in the serum of severe preeclampsia group was 180.62 鹵13.66 mg / L 103.32 鹵5.35 mg / L, respectively. There were significant differences in the relative expression of XIAPP VEGF in serum of severe preeclampsia group. There was a positive correlation between the expression of XIAPP VEGF in serum and the level of XIAP in the control group. The positive expression rates of placenta in mild and severe preeclampsia groups were 100% and 62%, respectively. There was significant difference between the mild preeclampsia group and the control group (蠂 2 / 17.391P 0.000), and between the severe preeclampsia group and the mild preeclampsia group, there was a significant difference between the severe preeclampsia group and the mild preeclampsia group. The positive expression rates of VEGF in placenta of mild and severe preeclampsia groups were 100% and 72%, respectively. The positive rates of VEGF in mild and severe preeclampsia groups were 100% and 72%, respectively. The positive expression rates of VEGF in mild and severe preeclampsia groups were significantly higher than those in control group. The difference was statistically significant (蠂 ~ 2 + 10.497) P ~ (0.001), and there was a significant difference between the severe group and the mild group (蠂 ~ (2 +) 9.269% P ~ (0.002). Conclusion: VEGF may be involved in the development of preeclampsia.
【學位授予單位】：廣西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R714.244

【共引文獻】

相關期刊論文 前8條

1 高燕;葉迎春;袁青;周云剛;黃黎;劉佳佳;;MALP-2對多形核中性細胞凋亡及其Bax蛋白表達的影響[J];重慶醫(yī)學;2012年14期

2 王素梅,唐卉;滋養(yǎng)細胞凋亡在妊娠期高血壓疾病發(fā)病機制方面的研究[J];廣西醫(yī)學;2005年03期

3 鄭宇;胡繼芬;李勵軍;;凋亡蛋白抑制因子在病理妊娠中的研究進展[J];海峽預防醫(yī)學雜志;2010年02期

4 盛霞玲;;妊娠高血壓綜合征胎盤細胞凋亡的研究[J];交通醫(yī)學;2006年04期

5 田曉艷;其木格;;胎盤細胞凋亡與妊娠期高血壓疾病關系的研究進展[J];內蒙古醫(yī)學院學報;2008年S2期

6 王云霞;劉萍;任力群;葛紹明;;重度子癇前期胎盤細胞凋亡及其基因的表達[J];中國婦幼健康研究;2007年06期

7 李詠梅;;重度妊娠高血壓綜合征胎盤組織中細胞凋亡的臨床觀察與研究[J];實用心腦肺血管病雜志;2012年11期

8 張z鴉

本文編號：1910864