本文選題：miRb + Sp��； 參考：《中南大學學報(醫(yī)學版)》2017年10期

【摘要】：目的:檢測miR23b和特異蛋白1(specific protein 1,Sp1)在卵巢子宮內膜異位癥中的表達和分布情況,探討二者在卵巢子宮內膜異位癥發(fā)生發(fā)展中的作用及意義。方法:qPCR檢測miR23b和Sp1 mRNA在異位內膜、在位內膜、正常內膜中的表達水平;免疫組織化學和Western印跡檢測Sp1蛋白在異位內膜、在位內膜、正常內膜的表達和分布情況。結果:MiR23b mRNA在異位內膜、在位內膜、正常內膜組中的表達水平依次增加(P0.05)。Sp1在子宮內膜間質細胞、腺上皮細胞中均有表達,主要表達在細胞核,少量表達在胞質中;Sp1 mRNA和蛋白在異位內膜、在位內膜、正常內膜中的表達均依次降低(P0.05)。MiR23b與Sp1 mRNA表達呈負相關(r= 0.526,P0.05)。結論:MiR23b和Sp1與卵巢子宮內膜異位癥的發(fā)生發(fā)展密切相關,二者可能通過相互拮抗,共同參與異位病灶的形成。
[Abstract]:Objective: to detect the expression and distribution of miR23b and specific protein 1(specific protein 1 / Sp1 in ovarian endometriosis and to explore the role and significance of them in the occurrence and development of ovarian endometriosis. Methods the expression and distribution of miR23b and Sp1 mRNA in ectopic, eutopic and normal endometrium were detected by 1: qPCR, and the expression and distribution of Sp1 protein in ectopic, eutopic and normal endometrium were detected by immunohistochemistry and Western blot. Results the expression of mRNA in ectopic endometrium, eutopic endometrium, normal endometrium and normal endometrium increased in turn, and the expression of P0.05U 路Sp1 in stromal cells and glandular epithelial cells of endometrium, mainly in the nucleus, was observed in both endometrium and glandular epithelium. The expression of Sp1 mRNA and protein in ectopic endometrium, eutopic endometrium and normal endometrium decreased in turn, and the expression of P0.05N. MiR23b was negatively correlated with Sp1 mRNA expression. Conclusion the ratio MiR23b and Sp1 are closely related to the occurrence and development of ovarian endometriosis. They may be involved in the formation of ectopic lesions through mutual antagonism.
【作者單位】： 中南大學湘雅醫(yī)院婦產科;
【基金】：中南大學湘雅醫(yī)院青年科研基金(2014Q09)~~
【分類號】：R711.71

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