本文選題：海洛因 + 孕鼠��； 參考：《西北師范大學(xué)學(xué)報(bào)(自然科學(xué)版)》2017年06期

【摘要】：研究母鼠注射海洛因?qū)ψ惺蟠竽X額葉結(jié)構(gòu)與相關(guān)蛋白表達(dá)的影響,選20只母鼠隨機(jī)分為空白對(duì)照組和海洛因1,2,3組,從受孕第10d開(kāi)始,各孕鼠分別腹腔連續(xù)注射生理鹽水和2.0,4.0,6.0g·L-1的海洛因溶液,直至孕鼠分娩后15d,于仔鼠出生5d,10d和15d分別稱量檢測(cè)仔鼠大腦的重量,用光學(xué)顯微鏡觀察其大腦額葉的顯微結(jié)構(gòu)變化,用免疫組化法檢測(cè)額葉皮層NGF和Caspase-3蛋白的表達(dá).結(jié)果表明,各海洛因組仔鼠大腦重量均顯著或極顯著低于空白對(duì)照組(P0.05或P0.01);海洛因組仔鼠額葉皮層組織結(jié)構(gòu)有不同程度的發(fā)育缺陷,外顆粒層、內(nèi)顆粒層、錐體細(xì)胞層和多形細(xì)胞層的細(xì)胞均出現(xiàn)空泡化和細(xì)胞凋亡現(xiàn)象,額葉皮層NGF表達(dá)增強(qiáng),Caspase-3蛋白的表達(dá)極顯著增強(qiáng)(P0.01).表明母鼠注射海洛因影響仔鼠大腦額葉組織的正常發(fā)育.
[Abstract]:To study the effect of heroin injection on the structure of frontal lobe and the expression of related proteins in the brain of young rats, 20 female rats were randomly divided into blank control group and heroin 1 + 2 + 3 group, starting from the 10th day of conception.Each pregnant rat was injected intraperitoneally with normal saline and 2.0 ~ 4.0 ~ 6.0 g 路L ~ (-1) heroin solution continuously until 15 days after delivery. The weight of the brain was measured on the 5th and 15th day after birth, and the microstructure of the frontal lobe of the newborn rat was observed by optical microscope.The expression of NGF and Caspase-3 in frontal cortex was detected by immunohistochemistry.The results showed that the brain weight of each heroin group was significantly lower than that of the blank control group (P0.05 or P0.01), and the structure of the frontal cortex of the heroin group had different degrees of developmental defects, including the outer granular layer and the inner granular layer.Vacuolation and apoptosis appeared in pyramidal cell layer and polymorphic cell layer, and the expression of Caspase-3 protein in frontal cortex increased significantly (P 0.01).The results showed that the injection of heroin affected the normal development of frontal lobe tissue.
【作者單位】： 西北師范大學(xué)生命科學(xué)學(xué)院;西北師范大學(xué)學(xué)報(bào)編輯部;
【基金】：甘肅省自然科學(xué)基金資助項(xiàng)目(1107RJZA141) 蘭州市社會(huì)發(fā)展計(jì)劃資助項(xiàng)目(2013-3-72,2014-1-105)
【分類(lèi)號(hào)】：R714

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