本文關(guān)鍵詞： 孕鼠 哺乳鼠 肝臟膽酸 法尼醇X受體 小異二聚體伴侶受體 膽固醇α-羥化酶　出處：《重慶醫(yī)學(xué)》2015年30期 　論文類型：期刊論文

【摘要】：目的研究健康SD鼠在妊娠和哺乳期肝臟膽酸代謝基因的變化。方法取孕10、14、19d及產(chǎn)后1、7、14、21d的大鼠肝臟,檢測(cè)膽酸水平和肝臟法尼醇X受體(FXR)、小異二聚體伴侶受體(SHP)、膽固醇7α-羥化酶(Cyp7α1)的表達(dá)。用Western blot法測(cè)Cyp7α1的蛋白表達(dá)情況。結(jié)果孕晚期健康大鼠,肝臟中膽酸及Cyp7α1表達(dá)不升高;哺乳期的大鼠,肝臟膽酸增加,FXR表達(dá)升高;在孕晚期及哺乳期靶基因SHP表達(dá)均高于對(duì)照組,且其表達(dá)高峰期是第孕19天,其值可達(dá)對(duì)照組的6倍之多。結(jié)論健康大鼠在產(chǎn)后肝臟膽酸合成增加,而孕期減少,可能與Cyp7α1、FXR及SHP表達(dá)有關(guān)。
[Abstract]:Objective to study the changes of bile acid metabolism genes in the liver of healthy SD rats during pregnancy and lactation. The levels of cholic acid and hepatic FXRX receptor FXRX, small heterodimer chaperone receptor (SHP) were measured. The expression of cholesterol 7 偽 -hydroxylase (Cyp7 偽 1). The expression of Cyp7 偽 1 protein was measured by Western blot method. The expression of cholic acid and Cyp7 偽 1 in liver was not increased. In lactating rats, cholic acid in liver increased the expression of FXR. The expression of target gene SHP in late pregnancy and lactation was higher than that in control group, and the peak expression of target gene was at the 19th day of gestation, which was 6 times higher than that in control group. Conclusion the synthesis of cholic acid in the liver of healthy rats increased after delivery. The decrease in pregnancy may be related to the expression of Cyp7 偽 1 FXR and SHP.
【作者單位】： 遵義醫(yī)學(xué)院第三附屬醫(yī)院婦產(chǎn)科;遵義醫(yī)學(xué)院藥理教研室暨貴州省基礎(chǔ)藥理重點(diǎn)實(shí)驗(yàn)室;美國(guó)堪薩斯大學(xué)醫(yī)學(xué)中心;
【基金】：貴州省科技廳國(guó)際合作項(xiàng)目[黔科合外G字(2009)70019] 貴州省教育廳研究生教育創(chuàng)新基地項(xiàng)目(023)
【分類號(hào)】：R714.255

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本文編號(hào)：1493163