正常腦老化過程中雙側(cè)海馬不同部位MRS差異性研究
發(fā)布時間：2018-08-23 14:42
【摘要】：目的：利用1H-MRS技術(shù)，評價正常腦老化過程中雙側(cè)海馬不同部位物質(zhì)代謝的變化情況，并進(jìn)一步探討代謝物濃度及代謝物濃度比值與年齡間的相關(guān)性，旨在探索海馬的生理老化規(guī)律，為海馬相關(guān)疾病的早期診斷及其治療奠定基礎(chǔ)。 方法：121名健康右利手志愿者，男62名，女59名，年齡20~89歲，平均48.7±16.5歲，行顱腦常規(guī)MRI、MRS檢查，按年齡分為青年組（20~44歲）、中年組（45~59歲）、老年組（60~89歲），每組約40人，行顱腦常規(guī)MRI、MRS檢查。人為確定感興趣區(qū)，通過掃描，機器自動測量出所需參數(shù)：NAA、Cho、Cr濃度及NAA/Cr、Cho/Cr等濃度比值。應(yīng)用SPSS13.0軟件進(jìn)行統(tǒng)計分析。采用t檢驗(分組、配對t檢驗)、方差分析(One-Way ANOVA)等統(tǒng)計方法分析不同性別、不同側(cè)別、年齡組間各統(tǒng)計指標(biāo)間的差異性，規(guī)定以P 0.05為差異有統(tǒng)計學(xué)意義，其中方差分析年齡組間差異性時兩兩年齡組間比較采用最小顯著差異(least significantdifference, LSD)t檢驗。最后應(yīng)用Pearson相關(guān)分析，進(jìn)一步分析海馬各部位代謝物NAA、Cho、Cr濃度與年齡的直線相關(guān)關(guān)系。 結(jié)果： 1雙側(cè)海馬頭、體（體1區(qū)、體2區(qū)）、尾部NAA濃度值與年齡存在負(fù)相關(guān)（P＜0.05）；雙側(cè)海馬頭、體（體1區(qū)、體2區(qū)）、尾部Cho、Cr濃度值與年齡不存在相關(guān)性(p＞0.05)。 2年齡組間差異性：雙側(cè)海馬頭、體（體1區(qū)、體2區(qū)）、尾部NAA老年組與青年組（P1-3）、中年組（P2-3）間存在統(tǒng)計學(xué)差異性，P＜0.05，且老年組明顯低于青年組及中年組，而青年組與中年組間P＞0.05，無統(tǒng)計學(xué)學(xué)意義；Cho及Cr在青、中、老年三組間兩兩組間比較結(jié)果均表現(xiàn)為P＞0.05，無統(tǒng)計學(xué)意義。雙側(cè)海馬頭部代謝物濃度比NAA/Cr在老年組與青年組（P1-3）、老年組與中年組間（P2-3）存在差異性，P＜0.05，且老年組明顯低于青年組及中年組，而青年組與中年組間P1-2＞0.05，，尚不能認(rèn)為青年組與中年組間NAA/Cr存在差異。雙側(cè)海馬體1區(qū)、左側(cè)海馬體2區(qū)、左側(cè)海馬尾部代謝物濃度比NAA/Cr結(jié)果顯示僅P1-3＜0.05，存在統(tǒng)計學(xué)差異性。海馬頭、體（體1區(qū)、體2區(qū)）、尾各部位各年齡組間Cho、Cr、Cho/Cr結(jié)果均顯示P＞0.05，無統(tǒng)計學(xué)意義。 3性別差異性：男性各代謝物質(zhì)NAA、Cho、Cr及其代謝物比值NAA/Cr、Cho/Cr與女性相比未見明顯統(tǒng)計學(xué)差異，p＞0.05。 4側(cè)別差異性：海馬頭部NAA、Cho、Cr、NAA/Cr、Cho/Cr均不存在側(cè)別差異（P＞0.05）；海馬體1區(qū)NAA、Cho、Cr存在側(cè)別差異性（P＜0.05）,且右側(cè)大于左側(cè)，而NAA/Cr、Cho/Cr不存在側(cè)別差異（P＞0.05）；海馬體2區(qū)NAA、Cho、Cr存在側(cè)別差異性（P＜0.05），且右側(cè)大于左側(cè)，而NAA/Cr、Cho/Cr不存在側(cè)別差異（P＞0.05)；海馬尾部NAA、Cho、Cr存在側(cè)別差異性（P＜0.05），且右側(cè)大，而NAA/Cr、Cho/Cr不存在側(cè)別差異（P＞0.05)。 結(jié)論： 1應(yīng)用MRS評估海馬代謝物質(zhì)隨年齡變化有無規(guī)律性是可行的。 2在海馬各部位均發(fā)現(xiàn)老年組NAA明顯減低，青年組與中年組間變化不大；而NAA/Cr結(jié)果與NAA結(jié)果存在差異性，證明NAA/Cr是NAA及Cr共同作用的結(jié)果，觀測神經(jīng)元及軸突變化時NAA較NAA/Cr更精準(zhǔn)；海馬同一部位不同年齡組間Cho、Cr、Cho/Cr不存在差異性。 3海馬頭、體（體1區(qū)、體2區(qū)）、尾部NAA值均隨年齡的增高而降低；Cho、Cr與年齡無此線性關(guān)系。 4海馬同一部位各代謝物及其代謝物濃度比值間均不存在性別差異性。 5海馬體1、體2區(qū)、尾部代謝物濃度存在側(cè)別差異，表現(xiàn)為右側(cè)＞左側(cè)，海馬體1區(qū)、體2區(qū)、尾部代謝物濃度比值不存在側(cè)別差異；海馬頭部代謝物濃度及代謝物濃度比值均不存在側(cè)別差異。
[Abstract]:Objective: To evaluate the metabolic changes of different parts of bilateral hippocampus during normal brain aging by 1H-MRS, and to explore the correlation between metabolite concentration and metabolite concentration ratio and age, so as to explore the physiological aging regularity of hippocampus and lay a foundation for early diagnosis and treatment of hippocampal related diseases.
Methods: 121 healthy right-handed volunteers, 62 males and 59 females, aged from 20 to 89 years, with an average of 48.7 (+ 16.5 years) underwent routine MRI and MRS examinations. They were divided into young group (20-44 years old), middle-aged group (45-59 years old), and old group (60-89 years old). Routine MRI and MRS examinations were performed on 40 subjects in each group. SPSS13.0 software was used for statistical analysis. T test (grouping, paired t test), one-way ANOVA and other statistical methods were used to analyze the differences of statistical indicators among different gender, different side, and age groups. It was stipulated that P 0.05 was the difference of statistical indicators. Meaning, variance analysis was used to compare the differences between age groups by least significant difference (LSD) t test. Finally, Pearson correlation analysis was used to further analyze the linear correlation between the concentrations of NAA, Cho, Cr and age in different parts of the hippocampus.
Result:
There was a negative correlation between the concentration of NAA in the head, body (body 1 area, body 2 area), tail and age (P < 0.05); there was no correlation between the concentration of Cho and Cr in the head, body (body 1 area, body 2 area), tail and age (p > 0.05).
The differences between the two-year-old groups were statistically significant (P The results showed that there was no significant difference between the two groups (P > 0.05). The concentration ratio of NAA / Cr in hippocampus was different between the old group and the young group (P 1-3), the old group and the middle-aged group (P < 0.05), and the old group was significantly lower than the young group and the middle-aged group. There were significant differences in NAA/Cr between middle-aged and middle-aged groups.The concentrations of metabolites in bilateral hippocampus 1 area, left hippocampus 2 area and left hippocampus tail area were only P1-3 < 0.05. The results of Cho, Cr and Cho/Cr in hippocampus head, body (body 1 area, body 2 area) and tail area showed no statistical significance.
Gender differences: There was no significant difference in the ratio of NAA, Cho, Cr and their metabolites NAA/Cr, Cho/Cr between male and female, P > 0.05.
There were no lateral differences in NAA, Cho, Cr, NAA/Cr and Cho/Cr in hippocampus head (P > 0.05); there were lateral differences in NAA, Cho and Cr in hippocampus 1 region (P < 0.05), and the right side was larger than the left side, but there was no lateral difference in NAA/Cr and Cho/Cr in hippocampus 2 region (P > 0.05); there were lateral differences in NAA, Cho and Cr in hippocampus 2 region (P < 0.05), and the right side was larger than the left side, and the right side was larger than the left side. There was no lateral difference in NAA/Cr and Cho/Cr (P > 0.05), and there was lateral difference in NAA, Cho and Cr in the tail of hippocampus (P < 0.05), but there was no lateral difference in NAA/Cr and Cho/Cr (P > 0.05).
Conclusion:
1 it is feasible to use MRS to assess whether there is regularity in the metabolism of hippocampal metabolites with age.
2. The NAA decreased significantly in all parts of the hippocampus, and there was no significant difference between young and middle-aged groups. The NAA/Cr results showed that NAA/Cr was the result of the interaction of NAA and Cr. The opposite sex.
The NAA values of hippocampus head, body (body 1, body 2) and tail decreased with age, but Cho, Cr had no linear relationship with age.
4 there was no gender difference in the ratios of metabolites and metabolites in the same part of the hippocampus.
5 There were lateral differences in the concentrations of metabolites in hippocampus 1, body 2 and tail, showing that there were no lateral differences in the concentrations of metabolites in right side > left side, hippocampus 1, body 2 and tail, and there were no lateral differences in the concentrations of metabolites in head and tail of hippocampus.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R338;R445.2
本文編號：2199377
[Abstract]:Objective: To evaluate the metabolic changes of different parts of bilateral hippocampus during normal brain aging by 1H-MRS, and to explore the correlation between metabolite concentration and metabolite concentration ratio and age, so as to explore the physiological aging regularity of hippocampus and lay a foundation for early diagnosis and treatment of hippocampal related diseases.
Methods: 121 healthy right-handed volunteers, 62 males and 59 females, aged from 20 to 89 years, with an average of 48.7 (+ 16.5 years) underwent routine MRI and MRS examinations. They were divided into young group (20-44 years old), middle-aged group (45-59 years old), and old group (60-89 years old). Routine MRI and MRS examinations were performed on 40 subjects in each group. SPSS13.0 software was used for statistical analysis. T test (grouping, paired t test), one-way ANOVA and other statistical methods were used to analyze the differences of statistical indicators among different gender, different side, and age groups. It was stipulated that P 0.05 was the difference of statistical indicators. Meaning, variance analysis was used to compare the differences between age groups by least significant difference (LSD) t test. Finally, Pearson correlation analysis was used to further analyze the linear correlation between the concentrations of NAA, Cho, Cr and age in different parts of the hippocampus.
Result:
There was a negative correlation between the concentration of NAA in the head, body (body 1 area, body 2 area), tail and age (P < 0.05); there was no correlation between the concentration of Cho and Cr in the head, body (body 1 area, body 2 area), tail and age (p > 0.05).
The differences between the two-year-old groups were statistically significant (P The results showed that there was no significant difference between the two groups (P > 0.05). The concentration ratio of NAA / Cr in hippocampus was different between the old group and the young group (P 1-3), the old group and the middle-aged group (P < 0.05), and the old group was significantly lower than the young group and the middle-aged group. There were significant differences in NAA/Cr between middle-aged and middle-aged groups.The concentrations of metabolites in bilateral hippocampus 1 area, left hippocampus 2 area and left hippocampus tail area were only P1-3 < 0.05. The results of Cho, Cr and Cho/Cr in hippocampus head, body (body 1 area, body 2 area) and tail area showed no statistical significance.
Gender differences: There was no significant difference in the ratio of NAA, Cho, Cr and their metabolites NAA/Cr, Cho/Cr between male and female, P > 0.05.
There were no lateral differences in NAA, Cho, Cr, NAA/Cr and Cho/Cr in hippocampus head (P > 0.05); there were lateral differences in NAA, Cho and Cr in hippocampus 1 region (P < 0.05), and the right side was larger than the left side, but there was no lateral difference in NAA/Cr and Cho/Cr in hippocampus 2 region (P > 0.05); there were lateral differences in NAA, Cho and Cr in hippocampus 2 region (P < 0.05), and the right side was larger than the left side, and the right side was larger than the left side. There was no lateral difference in NAA/Cr and Cho/Cr (P > 0.05), and there was lateral difference in NAA, Cho and Cr in the tail of hippocampus (P < 0.05), but there was no lateral difference in NAA/Cr and Cho/Cr (P > 0.05).
Conclusion:
1 it is feasible to use MRS to assess whether there is regularity in the metabolism of hippocampal metabolites with age.
2. The NAA decreased significantly in all parts of the hippocampus, and there was no significant difference between young and middle-aged groups. The NAA/Cr results showed that NAA/Cr was the result of the interaction of NAA and Cr. The opposite sex.
The NAA values of hippocampus head, body (body 1, body 2) and tail decreased with age, but Cho, Cr had no linear relationship with age.
4 there was no gender difference in the ratios of metabolites and metabolites in the same part of the hippocampus.
5 There were lateral differences in the concentrations of metabolites in hippocampus 1, body 2 and tail, showing that there were no lateral differences in the concentrations of metabolites in right side > left side, hippocampus 1, body 2 and tail, and there were no lateral differences in the concentrations of metabolites in head and tail of hippocampus.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R338;R445.2
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