本文選題：乳腺癌 + 同種移植動(dòng)物模型��； 參考：《中國(guó)醫(yī)學(xué)科學(xué)院學(xué)報(bào)》2015年06期

【摘要】：目的探討乳腺癌超聲造影(CEUS)增強(qiáng)模式與缺氧誘導(dǎo)因子1α(HIF-1α)表達(dá)及微血管密度(MVD)的關(guān)系。方法在22只小鼠體內(nèi)建立同種移植性乳腺癌,于接種后第4、6、7、8、9、10、11天隨機(jī)抓取3~4只小鼠進(jìn)行常規(guī)超聲及CEUS檢查,檢查結(jié)束后處死小鼠并使用免疫組織化學(xué)方法檢測(cè)腫瘤標(biāo)本中HIF-1α表達(dá)及MVD,分析CEUS增強(qiáng)模式與HIF-1α表達(dá)及MVD的關(guān)系。結(jié)果 22只小鼠乳腺癌中瘤體0.05 cm3組5例,CEUS表現(xiàn)為Ⅰ型增強(qiáng)模式,周邊環(huán)狀增強(qiáng),內(nèi)部無(wú)增強(qiáng)4例,Ⅱ型增強(qiáng)模式,周邊環(huán)狀增強(qiáng)并向病灶內(nèi)呈點(diǎn)線(xiàn)狀穿入1例;0.05~0.75 cm3組9例,多表現(xiàn)為Ⅲ型增強(qiáng)模式,病灶彌漫性增強(qiáng),均勻/不均勻5例;0.75 cm3組8例,多表現(xiàn)為Ⅳ型增強(qiáng)模式,周邊環(huán)狀增強(qiáng),內(nèi)部結(jié)節(jié)狀增強(qiáng)7例。3組瘤體在CEUS增強(qiáng)模式上差異有統(tǒng)計(jì)學(xué)意義(P0.01),Ⅰ~Ⅳ型CEUS增強(qiáng)模式與腫瘤大小相關(guān)(r=0.841,P0.05)。小鼠乳腺癌中HIF-1α表達(dá)與增強(qiáng)模式(Ⅰ~Ⅳ型)呈負(fù)相關(guān)(r=-0.596,P=0.003),與腫瘤大小無(wú)相關(guān)性(P0.05)。Ⅰ~Ⅳ型CEUS增強(qiáng)模式間MVD均值差異無(wú)統(tǒng)計(jì)學(xué)意義,MVD與腫瘤大小無(wú)相關(guān)性(P0.05)。結(jié)論 CEUS可動(dòng)態(tài)評(píng)估同種移植性小鼠乳腺癌的血管生成,腫瘤增強(qiáng)模式與腫瘤HIF-1α的表達(dá)水平相關(guān)。
[Abstract]:Objective to investigate the relationship between the enhanced mode of CEUs and the expression of hypoxia inducible factor-1 偽 (HIF-1 偽) and microvessel density (MVD) in breast cancer.Methods the allograft breast cancer was established in 22 mice. On the 4th day after inoculation, 34 mice were randomly picked up for routine ultrasound and CEUS examination.After examination, the mice were killed and the expression of HIF-1 偽 and MVD in tumor specimens were detected by immunohistochemical method. The relationship between CEUS enhancement mode and HIF-1 偽 expression and MVD were analyzed.There were 8 cases in 0. 75 cm3 group with diffuse enhancement, 8 cases in 0. 75 cm3 group, and 8 cases in 0. 75 cm3 group. Most of them showed type 鈪，

本文編號(hào)：1731557