本文選題：超順磁性氧化鐵納米顆粒　切入點：磁共振成像　出處：《重慶醫(yī)科大學(xué)學(xué)報》2015年09期

【摘要】：目的:嘗試采用磁共振成像(magnetic resonance imaging,MRI)監(jiān)測磁標記樹突狀細胞(dendritic cell,DC)歸巢引流淋巴結(jié)。方法:DC由小鼠骨髓細胞經(jīng)誘導(dǎo)分化獲得。探針為烷基化低分子量聚乙烯亞胺(PEI2k)包裹的超順磁性氧化鐵(superparamagnetic iron oxide,SPIO)納米顆粒,標記方法為成熟DC與探針共孵育過夜。收集細胞,行標記效果、細胞分子表型分析。將標記的成熟DC注入小鼠足底,行不同時間點MRI,觀察DC歸巢淋巴結(jié),72 h后,取乆窩淋巴結(jié)送病理檢查。結(jié)果:DC純度高達90%。普魯士蘭染色和激光共聚焦成像顯示探針位于細胞內(nèi)。流式細胞術(shù)(fluorescence activated cell sorting,FACS)分析表明,標記過程對DC的成熟表型無明顯影響。體內(nèi)MRI和淋巴結(jié)病理顯示,24 h后標記DC在淋巴結(jié)內(nèi)聚集。結(jié)論:采用N-alkylPEI2k/SPIO納米探針標記DC后,MRI可以清楚地監(jiān)測其在體內(nèi)歸巢淋巴結(jié)的情況。
[Abstract]:Objective: to monitor the homing and draining lymph nodes of dendritic cells (DC) by magnetic resonance imaging (MRI).Methods the mouse bone marrow cells were induced to differentiate into 1% DC.The probe consisted of superparamagnetic iron oxide nanoparticles coated with alkylated low molecular weight polyethylene imide (PEI2k). The labeling method was mature DC and probe incubated overnight.The cells were collected for labeling and molecular phenotypic analysis.The labeled mature DC was injected into the plantar of mice, and MRI was performed at different time points. After 72 hours of DC homing lymph nodes were observed, the lymph nodes were collected for pathological examination.As a result, the purity of 10% DC was as high as 90%.Prussian blue staining and laser confocal imaging showed that the probe was in the cell.Fluorescence activated cell sorting analysis showed that the labeling process had no significant effect on the mature phenotype of DC.In vivo MRI and lymph node pathology showed that DC were clustered in lymph nodes 24 h later.Conclusion: N-alkylPEI2k/SPIO labeled DC can clearly monitor the homing lymph nodes in vivo.
【作者單位】： 重慶醫(yī)科大學(xué)附屬兒童醫(yī)院檢驗科;四川大學(xué)國家生物醫(yī)學(xué)材料研究中心;重慶醫(yī)科大學(xué)附屬兒童醫(yī)院放射科;
【分類號】：R445.2

【共引文獻】

相關(guān)期刊論文 前2條

1 馬東霞;趙越;向瑩;劉斌;;小鼠骨髓源性樹突狀細胞的體外誘導(dǎo)擴增和鑒定[J];華中科技大學(xué)學(xué)報(醫(yī)學(xué)版);2013年04期

2 何秋山;張燕;何敬波;龔志敏;易鐵男;;培養(yǎng)條件對小鼠髓源性樹突狀細胞生物特性影響的觀察[J];中華腫瘤防治雜志;2013年18期

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