發(fā)布時間：2018-03-23 15:52

  本文選題：血管內(nèi)皮生長因子受體-　切入點：超聲　出處：《中國老年學(xué)雜志》2017年17期

【摘要】：目的探討血管內(nèi)皮細(xì)生長因子受體(VEGFR)-2分子探針的超聲分子成像評價小鼠下肢缺血性血管新生可行性。方法將10只實驗小鼠麻醉后結(jié)扎一側(cè)下肢股動脈制備下肢缺血模型,術(shù)后第7天,所有小鼠隨機經(jīng)尾靜脈注入攜抗小鼠VEGFR-2靶向微泡(Mb VEGFR-2)和攜同型抗體對照微泡(Mbc),并行超聲分子檢查,測量雙下肢的顯影強度(VI)值,并處死小鼠后對骨骼肌進(jìn)行免疫組化檢查。結(jié)果經(jīng)過8 min循環(huán)時間后,Mb VEGFR-2組小鼠可見明顯的超聲顯影,Mbc組小鼠可見輕度超聲顯影,但其顯影強度明顯弱于Mb VEGFR-2組;在非缺血小鼠下肢,經(jīng)過8 min循環(huán)時間后,Mb VEGFR-2組和Mbc組小鼠均未見明顯的超聲顯影;缺血下肢實驗小鼠Mb VEGFR-2的VI值明顯高于Mbc和非缺血下肢實驗小鼠(P0.01),缺血下肢實驗小鼠Mbc的VI值高于非缺血下肢實驗小鼠(P0.05),非缺血下肢骨骼肌VI值在Mb VEGFR-2和Mbc之間差異無統(tǒng)計學(xué)意義(P0.05);DAB染色結(jié)果顯示下肢缺血小鼠骨骼肌血管有VEGFR-2表達(dá),而下肢非缺血小鼠骨骼肌血管中未見VEGFR-2表達(dá)。結(jié)論采用攜VEGFR-2分子探針的超聲成像對缺血下肢新血管進(jìn)行造影可行,為評價缺血性血管新生提供了病理學(xué)依據(jù)。
[Abstract]:Objective to evaluate the feasibility of ultrasound molecular imaging with vascular endothelial fine growth factor receptor (VEGF) receptor VEGFR-2 molecular probe to evaluate ischemic angiogenesis in mice lower extremity. Methods Ten experimental mice were anesthetized and ligated one side of lower extremity femoral artery to make lower extremity ischemia model. On the 7th day after operation, all mice were randomly injected with anti-mouse VEGFR-2 targeting microbubbles Mb VEGFR-2) and Ctrip antibody control group MbFR-2. The development intensity of both lower extremities was measured by ultrasonic molecular examination. The skeletal muscle was examined by immunohistochemistry after the mice were killed. Results after 8 min of circulating time, the mice in Mb VEGFR-2 group could be seen mild ultrasound development in MBC group, but the development intensity was significantly lower than that in Mb VEGFR-2 group. In the lower extremities of non-ischemic mice, there was no obvious ultrasound imaging in Mb VEGFR-2 group and Mbc group after 8 min cycle time. The VI value of Mb VEGFR-2 in ischemic lower extremity mice was significantly higher than that in Mbc and non-ischemic lower extremity mice, the VI value of Mbc in ischemic lower limb mice was higher than that in non-ischemic lower extremity experimental mice, and the VI value of non-ischemic lower limb skeletal muscle was between Mb VEGFR-2 and Mbc. The results of DAB staining showed that there was VEGFR-2 expression in skeletal muscle of mice with lower extremity ischemia. The expression of VEGFR-2 was not found in the skeletal muscle vessels of non-ischemic mice. Conclusion Ultrasonography with VEGFR-2 molecular probe is feasible for evaluating ischemic vascularization of lower extremity, which provides a pathological basis for the evaluation of ischemic angiogenesis.
【作者單位】： 華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬武漢兒童醫(yī)院(武漢市婦幼保健院);
【分類號】：R445.1

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本文編號：1654130