斷尾對脂尾型綿羊肉用性能、脂肪沉積及肝臟轉錄組差異基因表達的影響
發(fā)布時間：2019-02-15 21:55
【摘要】：脂尾型綿羊是在特定環(huán)境下,由于長期的環(huán)境脅迫作用使其進化形成在尾部特異性貯存脂肪的功能,通過尾部脂肪的沉積與動員,能夠適應代謝能供給不斷盈虧變化的營養(yǎng)環(huán)境。為了探究阻斷脂尾型綿羊脂肪在尾部的特異性沉積對其肉用性能和脂肪沉積的影響,本研究選取蘭州大尾羊和蒙古羊各18只,分別隨機選取其中9只進行斷尾作為試驗組,未斷尾羊為對照組。飼喂至15月齡時,屠宰所有試驗用羊。測定試驗羊的屠宰性能,并對從胴體上剝離的脂肪進行稱重。本試驗還選取試驗羊12-13肋骨處的背最長肌為試驗材料,測定其肉品質指標。同時采集蘭州大尾羊的肝臟組織樣品,運用實時熒光定量聚合酶鏈式反應技術(RT-qPCR),對本課題組利用RNA-Seq技術分析出的斷尾處理下蘭州大尾羊的肝臟差異表達基因的結果進行篩選驗證,并將驗證結果與胴體脂肪沉積量做相關性分析。本研究結果總結如下:(1)斷尾有顯著提高蘭州大尾羊背膘厚和GR值的趨勢(P0.10),對蒙古羊的屠宰性能無顯著影響。屠宰性能在這兩個品種間具有顯著差異性。(2)斷尾有顯著降低蘭州大尾羊羊肉的失水率、剪切力以及提高pH1的趨勢(P0.10),對蒙古羊的羊肉品質無顯著影響。蘭州大尾羊的失水率和剪切力均顯著低于蒙古羊(P0.05),而熟肉率和pH1顯著高于蒙古羊(P0.05)。(3)斷尾蘭州大尾羊的臀部、睪丸、腸胃和內部的脂肪沉積量顯著升高,總脂肪沉積量顯著降低(P0.05)。斷尾蒙古羊的總脂肪沉積量顯著降低(P0.05)。脂肪沉積在這兩個品種間具有顯著差異性。(4)斷尾后的蘭州大尾羊SLC38A11和SMIM24基因的相對表達量顯著升高(P0.05),ETTA2和TGM3基因的相對表達量顯著下降(P0.05),TYMS基因的相對表達量有顯著下降的趨勢(P0.10),而PIM1基因的相對表達量無顯著性變化。(5)SLC38A11基因的相對表達量與未斷尾蘭州大尾羊腸胃、內部的脂肪沉積量呈極顯著正相關(P0.01),與尾臀部、腎周的脂肪沉積量呈顯著正相關(P0.05);SMIM24基因的相對表達量與斷尾蘭州大尾羊睪丸、腎周的脂肪沉積量呈顯著正相關(P0.05);EAAT2基因的相對表達量與斷尾蘭州大尾羊臀部、尾臀部的脂肪沉積量呈顯著正相關(P0.05);PIM1基因的相對表達量與未斷尾蘭州大尾羊臀部脂肪沉積量呈顯著負相關(P0.05)。
[Abstract]:Fat-tailed sheep evolve into tail-specific fat storage function in a specific environment because of long-term environmental stress, through the accumulation and mobilization of tail fat. Be able to adapt to metabolic energy supply of constantly changing nutritional environment. In order to investigate the effect of blocking fat deposition on meat performance and fat deposition in fat tail sheep, 18 large tail sheep and 18 Mongolian sheep were selected in this study, 9 of them were randomly selected as experimental group. Unbroken sheep were used as control group. All experimental sheep were slaughtered at the age of 15 months. The slaughter performance of the sheep was measured and the fat stripped from the carcass was weighed. The longissimus dorsi muscle at 12-13 ribs was selected as the test material to determine the meat quality index. At the same time, liver tissue samples of Lanzhou large tail sheep were collected and real-time quantitative polymerase chain reaction (RT-qPCR) was used. The results of liver differentially expressed genes of Lanzhou large tail sheep were screened and verified by RNA-Seq technique, and the correlation between the results and carcass fat deposition was analyzed. The results were summarized as follows: (1) the trend of increasing backfat thickness and GR value significantly (P0. 10), but had no significant effect on slaughter performance of Mongolian sheep. The slaughter performance was significantly different between the two breeds. (2) the cut tail significantly decreased the water loss rate, shear stress and the trend of increasing pH1 (P0.10), but had no significant effect on the mutton quality of Mongolian sheep. The water loss rate and shear stress of Lanzhou large tail sheep were significantly lower than that of Mongolian sheep (P0.05), while the cooked meat rate and pH1 were significantly higher than those of Mongolian sheep (P0.05). (3). The amount of fat deposition in stomach and intestines was significantly increased and the total fat deposition was significantly decreased (P 0.05). The total fat deposition decreased significantly (P0.05). There was significant difference in fat deposition between the two breeds. (4) the relative expression of SLC38A11 and SMIM24 genes increased significantly (P0.05), and the relative expression of ETTA2 and TGM3 genes decreased significantly (P0.05). The relative expression of TYMS gene tended to decrease significantly (P0.10), but the relative expression of PIM1 gene had no significant change. (5) the relative expression of SLC38A11 gene was significantly higher than that of unsevered Lanzhou large tail sheep. There was a significant positive correlation between internal fat deposition and tail buttocks and perirenal fat deposition (P0.01). The relative expression of SMIM24 gene was positively correlated with the amount of fat deposition in testis and perirenal tissues of Lanzhou large tail sheep (P0.05). The relative expression of EAAT2 gene was positively correlated with the fat deposition in the buttocks and buttocks of Lanzhou large tail sheep (P0.05). The relative expression of PIM1 gene was negatively correlated with the fat deposition in the buttocks of Lanzhou large tail sheep (P0.05).
【學位授予單位】：甘肅農業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S826
本文編號：2423733
[Abstract]:Fat-tailed sheep evolve into tail-specific fat storage function in a specific environment because of long-term environmental stress, through the accumulation and mobilization of tail fat. Be able to adapt to metabolic energy supply of constantly changing nutritional environment. In order to investigate the effect of blocking fat deposition on meat performance and fat deposition in fat tail sheep, 18 large tail sheep and 18 Mongolian sheep were selected in this study, 9 of them were randomly selected as experimental group. Unbroken sheep were used as control group. All experimental sheep were slaughtered at the age of 15 months. The slaughter performance of the sheep was measured and the fat stripped from the carcass was weighed. The longissimus dorsi muscle at 12-13 ribs was selected as the test material to determine the meat quality index. At the same time, liver tissue samples of Lanzhou large tail sheep were collected and real-time quantitative polymerase chain reaction (RT-qPCR) was used. The results of liver differentially expressed genes of Lanzhou large tail sheep were screened and verified by RNA-Seq technique, and the correlation between the results and carcass fat deposition was analyzed. The results were summarized as follows: (1) the trend of increasing backfat thickness and GR value significantly (P0. 10), but had no significant effect on slaughter performance of Mongolian sheep. The slaughter performance was significantly different between the two breeds. (2) the cut tail significantly decreased the water loss rate, shear stress and the trend of increasing pH1 (P0.10), but had no significant effect on the mutton quality of Mongolian sheep. The water loss rate and shear stress of Lanzhou large tail sheep were significantly lower than that of Mongolian sheep (P0.05), while the cooked meat rate and pH1 were significantly higher than those of Mongolian sheep (P0.05). (3). The amount of fat deposition in stomach and intestines was significantly increased and the total fat deposition was significantly decreased (P 0.05). The total fat deposition decreased significantly (P0.05). There was significant difference in fat deposition between the two breeds. (4) the relative expression of SLC38A11 and SMIM24 genes increased significantly (P0.05), and the relative expression of ETTA2 and TGM3 genes decreased significantly (P0.05). The relative expression of TYMS gene tended to decrease significantly (P0.10), but the relative expression of PIM1 gene had no significant change. (5) the relative expression of SLC38A11 gene was significantly higher than that of unsevered Lanzhou large tail sheep. There was a significant positive correlation between internal fat deposition and tail buttocks and perirenal fat deposition (P0.01). The relative expression of SMIM24 gene was positively correlated with the amount of fat deposition in testis and perirenal tissues of Lanzhou large tail sheep (P0.05). The relative expression of EAAT2 gene was positively correlated with the fat deposition in the buttocks and buttocks of Lanzhou large tail sheep (P0.05). The relative expression of PIM1 gene was negatively correlated with the fat deposition in the buttocks of Lanzhou large tail sheep (P0.05).
【學位授予單位】：甘肅農業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S826
【參考文獻】
相關期刊論文 前1條
1 閆振龍;溫建維;趙生國;朱燦燦;李路;韓志磊;王白雪;劉英;吳建平;;蘭州大尾羊尾巴的形態(tài)特征與斷尾方法研究[J];甘肅農業(yè)大學學報;2014年06期
，本文編號：2423733
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