【摘要】：【目的】為了提高鴨病毒性肝炎弱毒(DHV)活疫苗的耐熱性能�！痉椒ā窟x用蔗糖、普魯蘭糖、明膠、甘油、磷酸鹽等成分設(shè)計(jì)耐熱保護(hù)劑組方,根據(jù)配方的共晶點(diǎn)、塌陷溫度科學(xué)設(shè)計(jì)凍干程序,并比較了不同預(yù)凍方式對(duì)鴨病毒性肝炎活疫苗的效價(jià)影響�！窘Y(jié)果】耐熱保護(hù)劑配方A在37℃10 d、15 d病毒損失分別為0.35、0.61 Lg;對(duì)快凍、慢凍以及退火3種不同的凍干方式進(jìn)行比較,發(fā)現(xiàn)對(duì)鴨肝炎病毒耐熱損失由大到小依次為:退火慢凍快凍;疫苗長(zhǎng)期保存期試驗(yàn)結(jié)果表明,可在2~8℃可保存2年;采用10倍劑量免疫2日齡雛鴨均未出現(xiàn)死亡,且精神飲食體重增長(zhǎng)均正常。【結(jié)論】采用A配方與快速凍干凍方式的耐熱性能最好,延長(zhǎng)保存期并且提高了耐熱性能,為動(dòng)物疫苗耐熱新劑型的研發(fā)提供技術(shù)支撐。
[Abstract]:[objective] in order to improve the heat resistance of duck viral hepatitis attenuated (DHV) vaccine, sucrose, pullulanose, gelatin, glycerol, phosphate and other components were selected to design the formula of heat resistance protectant, according to the eutectic point of the formula, Collapse temperature scientifically designed freeze-drying program, and compared the effects of different pre-freezing methods on the titer of live duck viral hepatitis vaccine. [results] the virus loss of heat-resistant protective agent formula A at 37 鈩，

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