【摘要】：乳房炎是奶牛養(yǎng)殖過程中最常見的一種多發(fā)性疾病,.不僅對(duì)患病奶牛的產(chǎn)乳量、產(chǎn)乳品質(zhì)有很大的影響,而且嚴(yán)重影響奶牛的產(chǎn)后發(fā)情時(shí)間以及妊娠時(shí)間,還會(huì)危及人類健康。本研究利用寧夏地區(qū)所患臨床型以及隱性型乳房炎的奶牛為來源,采集乳樣分離疑似沙門氏菌菌株,分別進(jìn)行了奶牛乳房炎源沙門氏菌的分離,生化鑒定試驗(yàn),相關(guān)基因的PCR檢測(cè)方法的建立與應(yīng)用；奶牛乳房炎源沙門氏菌的血清型的鑒定分析；奶牛乳房炎源沙門氏菌的耐藥性檢測(cè)試驗(yàn)。1.奶牛乳房炎源沙門氏菌的分離,生化鑒定試驗(yàn),相關(guān)基因的PCR(聚合酶鏈?zhǔn)椒磻?yīng))檢測(cè)方法的建立與應(yīng)用：利用傳統(tǒng)方法,沙門氏菌鑒定培養(yǎng)基分離鑒定,共得到疑似分離株12株；根據(jù)相關(guān)報(bào)道,設(shè)計(jì)出五種鑒定沙門細(xì)菌常用引物hns、invA、 hilA、hut、sms,建立PCR方法,對(duì)奶牛乳房炎源沙門氏菌DNA(脫氧核糖核酸)進(jìn)行PCR擴(kuò)增并進(jìn)行確認(rèn)。最終,應(yīng)用此方法鑒定出本次試驗(yàn)收集到的12株奶牛乳房炎源沙門氏菌疑似株中,12株均為沙門氏菌。2.奶牛乳房炎源沙門氏菌的血清型的鑒定分析：對(duì)現(xiàn)有的12株奶牛乳房炎源沙門氏菌進(jìn)行O抗原血清學(xué)鑒定。鑒定結(jié)果表明,12株奶牛乳房炎源沙門氏菌株優(yōu)勢(shì)血清型為04(66.7%)、04,12(66.7%)、09,12(33.3%),以多價(jià)為主。3.奶牛乳房炎源沙門氏菌的耐藥性檢測(cè)試驗(yàn)。對(duì)12株奶牛乳房炎源沙門氏菌進(jìn)行耐藥性試驗(yàn),發(fā)現(xiàn)多株沙門氏菌出現(xiàn)多重耐藥性。耐藥性高于50%有NAL(萘啶酸)、AMP(氨芐西林)、CFI(頭孢唑啉)、STR(鏈霉素)、TET(四環(huán)素)、DC(多西環(huán)素)、AZI(阿奇霉素)一共七種,占本實(shí)驗(yàn)使用抗生素的58.3%,耐藥率低于20%有NOR(諾氟沙星)、CRO(頭孢曲松鈉)、CTX(頭孢噻肟)、AKN(丁胺卡那)占本實(shí)驗(yàn)總是用抗生素種類的33.3%。各株細(xì)菌對(duì)抗生素的耐藥性存在較大差異。
[Abstract]:Mastitis is one of the most common diseases in dairy cattle breeding. It not only has a great influence on milk yield and milk quality of sick cows, but also seriously affects the time of postpartum estrus and pregnancy time of dairy cows, and also endangers human health. In this study, milk samples were collected from clinical and recessive mastitis cows in Ningxia area to isolate the suspected Salmonella strains, and the isolation and biochemical identification of Salmonella from dairy mastitis were carried out. Establishment and application of PCR detection method for related genes; Identification and analysis of serotypes of salmonella from mastitis in dairy cattle; drug resistance test of salmonella from mastitis in dairy cattle. 1. Isolation of Salmonella from Bovine mastitis, biochemical Identification Test, Establishment and Application of PCR (Polymerase chain reaction) method for Detection of related genes: isolation and Identification of Salmonella by traditional methods, A total of 12 suspected isolates were obtained. According to relevant reports, five kinds of primers hns,invA, hilA,hut,sms, were designed to establish PCR method to amplify and confirm DNA (deoxyribonucleic acid) of Salmonella mastitis. Finally, this method was used to identify 12 suspected strains of salmonella from cow mastitis collected in this experiment, 12 of which were salmonella. Identification and analysis of serotypes of Salmonella mastitis in dairy cattle: 12 strains of Salmonella mastitis were serologically identified by O antigen. The results showed that the dominant serotypes of 12 strains of salmonella from mastitis of dairy cattle were 04 (66.7%), 04 (66.7%), 09 (33.3%), respectively. Drug Resistance Test of Salmonella from mastitis in Dairy cows. The drug resistance of 12 strains of Salmonella from mastitis of dairy cattle was tested and many strains of salmonella were found to have multiple drug resistance. More than 50% of drug resistance was found in seven NAL (), AMP (nalididinate, ampicillin,), CFI (, cefazolin,), STR (, streptomycin,), TET (, tetracycline,), DC (, doxycycline,), AZI (, azithromycin). NOR (norfloxacin), CRO (ceftriaxone sodium), CTX (cefotaxime), AKN (butyrate) accounted for 33.3% of the antibiotics used in this experiment. There were significant differences in antibiotic resistance among different strains of bacteria.
【學(xué)位授予單位】：寧夏大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：S858.23

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