【摘要】：本試驗旨在調(diào)查北京地區(qū)仔豬腹瀉的原因及豬源大腸桿菌血清型、毒力因子的分布情況。收集北京京郊地區(qū)仔豬腹瀉樣本400份,應用含2%血清的TSA培養(yǎng)基分離大腸桿菌,通過血清型鑒定試驗和毒力因子檢測驗證O抗原。結果顯示,400份樣本中分離到64株大腸桿菌,定型42株。其主要為O101型(18.7%)、O64型(12.5%)、O8型(10.9%)、O20型(10.9%)、O45型(4.7%)、O149型(4.7%)、O2型(1.6%)、O89型(1.6%)8種血清型,其攜帶的毒力因子為STa、Stx2e、astA和eaeA等。結果表明,引起京郊仔豬腹瀉的大腸桿菌主要有8個血清型,其中O101型發(fā)病比例最高。其攜帶的毒力因子主要為astA和eaeA,占全部菌株的50%以上,STa和Stx2e因子占全部菌株的30%以上。本試驗結果將為今后京郊地區(qū)仔豬腹瀉病的防控提供有效的數(shù)據(jù)支持。
[Abstract]:The aim of this study was to investigate the cause of piglet diarrhea and the distribution of serotype and virulence factor of Escherichia coli in Beijing area. A total of 400 samples of piglets with diarrhea were collected from Beijing suburb of Beijing. Escherichia coli was isolated by TSA medium containing 2% serum. O antigen was verified by serotype identification test and virulence factor test. The results showed that 64 strains of Escherichia coli were isolated from 400 samples and 42 strains were identified. O101 (18.7%), O64 (12.5%), O8 (10.9%), O20 (10.9%), O45 (4.7%), O149 (4.7%), O2 (1.6%), The virulence factors of O 89 (1.6%) were STa,Stx2e,astA and eaeA. The results showed that there were mainly 8 serotypes of Escherichia coli causing diarrhea of piglets in Jingjiao, among which O101 had the highest incidence. AstA and eaeA, accounted for more than 50% of the total strains, and STa and Stx2e accounted for more than 30% of the total strains. The results of this study will provide effective data for the prevention and control of piglet diarrhea in Beijing suburb.
【作者單位】： 北京農(nóng)業(yè)職業(yè)學院;中國人民解放軍軍事醫(yī)學科學院;內(nèi)蒙古農(nóng)業(yè)大學;
【基金】：北京市“菜籃子”科技能力提升工程項目 北京農(nóng)業(yè)職業(yè)學院院級項目
【分類號】：S858.28

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