【摘要】：新疆伊犁某地區(qū)一些馬群中不斷出現(xiàn)以全身多發(fā)性膿腫為特征的患病馬匹,為了查明其是否為馬腺疫,以及由何種馬鏈球菌感染所致,無菌采集典型患病馬匹膿汁樣品,常規(guī)THB增菌,綿羊鮮血平板劃線分離單菌落后,用透射電鏡對分離菌的微觀形態(tài)進行觀察,PCR對其fneB基因進行克隆與序列分析,最后用生化試驗進行驗證。結果表明,從12匹患病馬的膿腫組織中,共采集了25份樣品,從中分離鑒定出來源于不同患病馬的2株馬鏈球菌馬亞種菌株,這2株菌的形態(tài)均符合鏈球菌的特點;基因克隆測序結果表明,其中1株與英國源的獸疫亞種H70和馬亞種4047核苷酸同源性較高,均達97%~98%,另1株與英國源的馬亞種4047和瑞典源某馬亞種核苷酸同源性較高,均達98%~99%。這2株菌之間核苷酸序列同源性達98.3%,氨基酸同源性則達99.3%。馬鏈球菌馬亞種是引起伊犁某地區(qū)這些患馬全身多發(fā)性膿腫的主要致病菌,它們可能來源于之前從歐洲和美洲引進的馬匹,提示在進行疫病防控工作時,要特別注意海關檢疫,積極防止疫病的傳入。
[Abstract]:In a certain area of Yili, Xinjiang, some horses have been found to be sick with multiple abscesses. In order to find out whether the horses are infected with adenosis and by what kind of Streptococcus equine infection, aseptic samples of pus from a typical sick horse are collected. The micromorphology of the isolated bacteria was observed by transmission electron microscope (TEM), the fneB gene was cloned and sequenced by PCR, and the results were verified by biochemical test. The results showed that 25 samples were collected from the abscess tissues of 12 diseased horses. Two strains of Streptococcus equine Maya strains from different diseased horses were isolated and identified, and the morphology of the two strains were all in accordance with the characteristics of streptococcus. The results of gene cloning and sequencing showed that one strain had a high nucleotide homology with 4047 nucleotides of H70 and Maya, and the other had high nucleotide homology with Maya 4047 and Maya from Sweden. The nucleotide sequence homology and amino acid homology between the two strains were 98.3 and 99.3 respectively. The Maya strain of Streptococcus equi is the main pathogen causing multiple abscesses of horses in a certain area of Yili. They may have originated from horses imported from Europe and America, suggesting that special attention should be paid to customs quarantine in the prevention and control of epidemic diseases. Actively prevent the introduction of disease.
【作者單位】： 新疆農業(yè)大學動物醫(yī)學學院;新疆伊犁種馬場;
【基金】：國家科技支撐計劃項目(2012BAD46B02)
【分類號】：S852.611

【相似文獻】

相關期刊論文 前5條

1 楊建德;劉燕霏;李本強;;馬鏈球菌抗吞噬蛋白的鑒定[J];黑龍江畜牧獸醫(yī);2010年17期

2 Magne Bisgaard;Lise Hedegaard;Mark S.Chadfield;李永清;;多動物鏈球菌——一種新的禽病原體[J];中國家禽;2007年20期

3 劉燕霏;楊建德;于泓洋;;馬鏈球菌重組IdeE蛋白功能分析[J];黑龍江畜牧獸醫(yī);2013年17期

4 劉杰;楊建德;Timoney John;;馬鏈球菌Se39.612蛋白的克隆與表達[J];中國預防獸醫(yī)學報;2007年03期

5 王國勤;姜慧嬌;江航;蘇艷;;馬鏈球菌馬亞種新疆株ZX表面蛋白的提取及免疫原性分析[J];新疆農業(yè)大學學報;2014年03期

相關碩士學位論文 前4條

1 王國勤;馬鏈球菌馬亞種新疆分離株菌體蛋白的粗提取及免疫效果初步評估[D];新疆農業(yè)大學;2015年

2 阿依德古力·哈德依汗;中西藥對三種細菌的藥敏實驗及動物防治實驗[D];新疆農業(yè)大學;2015年

3 劉東宇;馬鏈球菌馬亞種間接ELISA方法的建立與應用[D];黑龍江八一農墾大學;2017年

4 李芳;馬鏈球菌獸疫亞種多重PCR檢測方法建立及應用[D];南京農業(yè)大學;2008年

，

本文編號：2278472