【摘要】：【目的】篩選出適合柑橘皮渣青貯的乳酸菌,以獲得柑橘皮渣青貯制劑最佳菌株。【方法】采用平板劃線法從自然發(fā)酵的柑橘皮渣和添加秸稈發(fā)酵的柑橘皮渣中初步分離乳酸菌,對其進行形態(tài)學和生理生化鑒定;再通過產(chǎn)酸性試驗、耐酸耐堿性試驗、生長曲線、產(chǎn)有機酸情況等進行優(yōu)良菌的復篩及其種屬鑒定。以直接青貯為對照,利用優(yōu)良菌發(fā)酵青貯柑橘皮渣,分析測定皮渣品質(zhì)。【結(jié)果】初選的12株乳酸菌中有7株為乳桿菌屬(Lactobacillus)細菌,5株為片球菌屬(Pediococcus)細菌。經(jīng)過產(chǎn)酸性試驗、耐酸耐堿性試驗、產(chǎn)有機酸情況等篩選出1株優(yōu)良菌株Q5,該菌株經(jīng)16SrDNA鑒定為植物乳桿菌。該菌株產(chǎn)酸快(pH可下降到3.48),發(fā)酵2h即可進入對數(shù)生長期,耐酸耐堿能力強,主要產(chǎn)乳酸和乙酸。利用該菌青貯的柑橘皮渣粗蛋白含量等有所提高,達到廣譜青貯劑的水平,優(yōu)于直接青貯�！窘Y(jié)論】菌株Q5是適合青貯柑橘皮渣的優(yōu)良菌,可作為柑橘皮渣青貯的菌種來源。
[Abstract]:[objective] to screen lactic acid bacteria suitable for citrus husk silage, In order to obtain the best strain of citrus peel silage preparation. [methods] Lactic acid bacteria were isolated from naturally fermented citrus peel dregs and citrus peel dregs fermented with straw by plate crossing method, and were identified by morphology, physiology and biochemistry. Through acid production test, acid and alkali tolerance test, growth curve, organic acid production conditions and so on to screen the fine bacteria and their species identification. Using direct silage as control, the quality of citrus peel residue was determined by fermentation of citrus peel dregs by fine bacteria. [results] among the 12 strains of lactic acid bacteria, 7 were Lactobacillus (Lactobacillus) bacteria and 5 were (Pediococcus) bacteria. An excellent strain Q5 was screened by acid production test, acid tolerance test and organic acid production test. The strain was identified as Lactobacillus plantarum by 16SrDNA. The strain can produce acid quickly (pH can be reduced to 3.48) and enter logarithmic growth period after 2 hours fermentation. The strain has strong acid- and alkali-tolerant ability and mainly produces lactic acid and acetic acid. The crude protein content of citrus peel was increased, which reached the level of broad-spectrum silage, which was superior to that of direct silage. [conclusion] strain Q5 is an excellent strain suitable for silage citrus peel silage and can be used as the source of citrus peel silage.
【作者單位】： 重慶工商大學環(huán)境與生物工程學院;重慶工商大學催化與功能有機分子重慶市重點實驗室;
【基金】：國家級星火計劃重點項目(2014GA81101) 重慶市研究生創(chuàng)新型科研項目(CYS14173)
【分類號】：S816.53

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