【摘要】：為進(jìn)一步降低冷凍解凍過(guò)程對(duì)山羊精子的理化損傷,本試驗(yàn)系統(tǒng)檢驗(yàn)了肌醇類(lèi)冰晶抑制劑(SIB)和海藻糖對(duì)山羊精子的冷凍保護(hù)效果。采用假陰道法采集6只云南黑山羊精液,離心洗滌去除精漿后和冷凍稀釋液混合,經(jīng)4℃平衡、液氮?dú)庀囝A(yù)凍后直接投入液氮保存。解凍后檢測(cè)精子活力、頂體、質(zhì)膜以及低滲耐受性。同時(shí)采用透射電鏡(TEM)觀察冷凍對(duì)精子超微結(jié)構(gòu)的影響。結(jié)果表明:1,4-環(huán)己二醇(1,4-CHD)和海藻糖處理組精子活力(41.35%±8.14%和43.59%±6.23%)、頂體完整性(62.73%±7.62%和64.29%±7.81%)和低滲耐受性(40.29%±7.41%和39.79%±6.58%)均顯著高于對(duì)照組(35.97%±6.21%、55.46%±8.91%和34.17%±5.68%,P0.05),且海藻糖可以有效抑制冷凍解凍過(guò)程導(dǎo)致的精子凋亡。流式分析結(jié)果表明,1,4-CHD不能降低冷凍對(duì)山羊精子質(zhì)膜完整性和磷脂酰絲氨酸(PS)分布的損傷,但是海藻糖可以顯著降低冷凍解凍過(guò)程中對(duì)山羊精子質(zhì)膜的損傷。電鏡結(jié)果表明,冷凍對(duì)精子質(zhì)膜和頂體等結(jié)構(gòu)損傷嚴(yán)重,解凍后部分精子質(zhì)膜發(fā)生腫脹、脫落或斷裂等現(xiàn)象。而且,頂體外膜存在脫落現(xiàn)象,頂體內(nèi)容物發(fā)生部分甚至完全泄漏。電鏡結(jié)果進(jìn)一步證實(shí)海藻糖對(duì)冷凍精子質(zhì)膜的保護(hù)效果要優(yōu)于1,4-環(huán)己二醇�？傊�,肌醇類(lèi)SIB和海藻糖可以降低山羊精子的冷凍損傷,但相對(duì)于海藻糖而言,肌醇類(lèi)SIB并不能降低冷凍對(duì)山羊精子質(zhì)膜的損傷。
[Abstract]:In order to further reduce the physicochemical damage of goat spermatozoa during freezing and thawing, the effects of inositol ice crystal inhibitor (SIB) and trehalose on goat spermatozoa were tested. Semen of 6 Yunnan black goats was collected by pseudovaginal method. Seminal plasma was removed by centrifugal washing and mixed with frozen diluent. After 4 鈩，

本文編號(hào)：2220059