【摘要】：本文旨在研究短期熱量限制對(duì)雄性大鼠睪丸形態(tài)和抗氧化能力的影響。將20只6周齡Wistar雄性大鼠隨機(jī)分為對(duì)照組(C)和短期熱量限制組(CR)(n=10),對(duì)照組自由采食,熱量限制組按對(duì)照組食物消耗量的60%進(jìn)行飼喂。連續(xù)飼養(yǎng)7周后稱重,觀察精神狀態(tài),處死,采集睪丸組織。對(duì)大鼠睪丸組織進(jìn)行HE染色、緊密連接蛋白(ZO-1)免疫熒光染色;通過生化技術(shù)測(cè)定超氧化物歧化酶、谷胱甘肽過氧化物酶活力及過氧化產(chǎn)物丙二醛含量;熒光定量PCR檢測(cè)ZO-1、超氧化酶歧化酶、過氧化氫酶、谷胱甘肽巰基轉(zhuǎn)移酶和轉(zhuǎn)錄因子Nrf2 mRNAs的表達(dá)變化。與對(duì)照組相比,短期熱量限制組大鼠體重上升趨勢(shì)減緩,精神狀態(tài)好于對(duì)照組,曲細(xì)精管生長(zhǎng)良好,基膜完整,管內(nèi)各級(jí)生精細(xì)胞排列整齊,生精上皮普遍可見有6-8層細(xì)胞。短期熱量限制組與對(duì)照組相比ZO-1 mRNAs表達(dá)顯著升高,同時(shí)在免疫熒光結(jié)果中觀察到ZO-1蛋白有較強(qiáng)的陽(yáng)性反應(yīng)。此外,短期熱量限制組睪丸組織相較于對(duì)照組丙二醛含量顯著降低(P0.05),超氧化酶歧化酶和谷胱甘肽過氧化物酶活力顯著升高(P0.05);短期熱量限制組相較于對(duì)照組抗氧化編碼基因SOD、CAT、GST和Nrf2 mRNAs水平顯著升高(P0.05)。短期熱量限制可以促進(jìn)大鼠睪丸支持細(xì)胞間的緊密連接,改善精子發(fā)育微環(huán)境,提高大鼠睪丸組織的抗氧化能力,減少自由基對(duì)細(xì)胞的毒性作用,改善大鼠生殖機(jī)能。
[Abstract]:The purpose of this study was to study the effects of short-term caloric restriction on testicular morphology and anti-oxidation in male rats. Twenty 6-week-old male Wistar rats were randomly divided into two groups: control group (C) and short-term caloric restriction group (CR) (10). The control group was fed freely, and the caloric restriction group was fed with 60% of the food consumption of the control group. After 7 weeks of continuous feeding, weight, mental state, death and testicular tissue were observed. HE staining and ZO-1 immunofluorescence staining were performed on rat testis, superoxide dismutase (SOD), glutathione peroxidase (GSH) activity and malondialdehyde (MDA) content were determined by biochemical technique. The expression of ZO-1, superoxidase dismutase, catalase, glutathione mercaptotransferase and transcription factor Nrf2 mRNAs were detected by fluorescence quantitative PCR. Compared with the control group, the short-term caloric restriction group showed a slower increase in body weight and better mental state. The seminiferous tubules grew well, the basement membrane was intact, the spermatogenic cells of all levels in the tube were arranged neatly, and 6-8 layers of spermatogenic cells were found in the spermatogenic epithelium. Compared with the control group, the expression of ZO-1 mRNAs was significantly increased in the short-term caloric restriction group, and the positive reaction of ZO-1 protein was observed in the immunofluorescence results. In addition, Compared with the control group, the content of malondialdehyde in testis of short-term caloric restriction group was significantly lower (P0.05), the activities of superoxidase dismutase (SOD) and glutathione peroxidase (Glutathione peroxidase) were significantly increased (P0.05), and the antioxidant coding unit of short-term caloric restriction group was higher than that of control The levels of SOD,CAT,GST and Nrf2 mRNAs increased significantly (P0.05). Short-term caloric restriction can promote the tight connection between Sertoli cells, improve the microenvironment of spermatozoa development, improve the antioxidant ability of rat testis, reduce the toxicity of free radicals to cells, and improve the reproductive function of rats.
【作者單位】： 南京農(nóng)業(yè)大學(xué)動(dòng)物醫(yī)學(xué)院;
【基金】：中央高�；緲I(yè)務(wù)費(fèi)自主創(chuàng)新重點(diǎn)項(xiàng)目(KYZ201526)
【分類號(hào)】：S814

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