【摘要】：本試驗(yàn)旨在研究益生菌大腸桿菌Nissle 1917(Ec N)抗逆性能、豬腸上皮細(xì)胞黏附率及抑菌效果。采用體外法對Ec N進(jìn)行生長曲線繪制和耐酸、耐膽鹽、耐熱性能的測定;以豬腸上皮細(xì)胞IPEC-J2細(xì)胞為體外細(xì)胞模型,考察了Ec N對該細(xì)胞的黏附率以及對致病菌大腸桿菌K88的黏附抑制率;同時(shí)通過蛋白質(zhì)印跡法檢測了Ec N對IPEC-J2細(xì)胞β-防御素-2和Toll樣受體4的水平的影響。結(jié)果表明:1)Ec N對高酸、高膽鹽和高溫環(huán)境具有一定耐受能力。2)Ec N對IPEC-J2細(xì)胞的黏附作用以對數(shù)期最佳,黏附率達(dá)33.96%,顯著高于遲緩期、穩(wěn)定期和衰亡期(P0.05)。3)Ec N對致病菌大腸桿菌K88具有良好的抑制效果,黏附抑制率達(dá)87.84%。4)Ec N還能上調(diào)IPEC-J2細(xì)胞β-防御素-2和Toll樣受體4水平。結(jié)果提示,益生菌Ec N具有較好的抗逆性能,能夠良好地黏附豬腸上皮細(xì)胞,對致病菌大腸桿菌K88具有良好的抑制作用。
[Abstract]:The aim of this study was to study the stress resistance of probiotic Escherichia coli Nissle 1917 (Ec N), the adhesion rate of porcine intestinal epithelial cells and the bacteriostatic effect. The growth curve of Ec N was plotted in vitro, and the acid resistance, bile salt resistance and heat resistance were measured. The porcine intestinal epithelial cells (IPEC-J2 cells) were used as cell models in vitro. The adhesion rate of Ec N to this cell line and the adhesion inhibition rate to Escherichia coli K88 were investigated, and the effects of Ec N on 尾 -defensin-2 and Toll like receptor 4 levels in IPEC-J2 cells were detected by Western blotting. The results showed that) Ec N had a certain tolerance to high acid, high bile salt and high temperature. 2) Ec N had the best adhesion to IPEC-J2 cells in logarithmic phase, and the adhesion rate was 33.96, which was significantly higher than that in delayed phase. The inhibition rate of adhesion to Escherichia coli K88 was 87.84.4) Ec N and up-regulated the levels of 尾 -defensin -2 and Toll like receptor 4 in IPEC-J2 cells. The results showed that probiotics Ec N had good resistance to stress, could adhere to porcine intestinal epithelial cells, and had a good inhibitory effect on Escherichia coli K88.
【作者單位】： 西南大學(xué)動(dòng)物科技學(xué)院生物飼料與分子營養(yǎng)室驗(yàn)室;
【基金】：重慶市自然科學(xué)基金(cstc2016jcyjA1414) 西南大學(xué)基本科研業(yè)務(wù)費(fèi)(XDJK2015D005) 國家973重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃項(xiàng)目(2013CB127303) 國家948項(xiàng)目(2015Z74)
【分類號】：S816

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