【摘要】：為了探究百里香酚對山羊子宮內(nèi)膜上皮細胞(goat endometrial epithelial cells,g EECs)的毒性作用,采用不同濃度的百里香酚作用于g EECs后,觀測其對細胞生長曲線,細胞形態(tài)學(xué)變化,以及細胞膜結(jié)構(gòu)完整性的影響。結(jié)果表明,百里香酚對EECs的毒性作用與其濃度呈劑量依賴效應(yīng)(y=-0.008x+1.2202,R~2=0.9767),即隨著藥物濃度的增加細胞的增殖抑制率增大,藥物對細胞的半數(shù)增殖抑制率(IC50)為100μg/m L。當50、100μg/m L的百里香酚作用于EECs時,g EECs的細胞核、細胞漿的形態(tài)均未產(chǎn)生明顯變化,但當150μg/m L的百里香酚作用于g EECs時,細胞膜破裂,細胞死亡;而且藥物濃度≥100μg/m L時細胞LDH的釋放量顯著高于正常細胞組,g EECs細胞膜的完整性受到破壞。百里香酚作用于山羊子宮內(nèi)膜上皮細胞后呈現(xiàn)一定的毒性作用,且毒性作用和藥物的濃度有關(guān)。百里香酚作用于g EECs的安全范圍在0~100μg/m L,研究結(jié)果為百里香酚治療奶牛等經(jīng)濟動物的炎癥性疾病的臨床用藥濃度提供理論依據(jù)。
[Abstract]:In order to investigate the toxicity of thyme to (goat endometrial epithelial cells g EECs in goat endometrial epithelial cells, the growth curve and morphological changes of the cells were observed after treated with different concentrations of thymol on g EECs. And the effect of cell membrane structural integrity. The results showed that the toxic effect of thyme phenol on EECs was dose-dependent (YP0. 008x 1. 2202RX 0.9767), that is, with the increase of drug concentration, the half proliferation inhibition rate (IC50) of thymol on EECs was 100 渭 g / mL. When 50100 渭 g / mL thymol acted on the nucleus of EECs, the morphology of cytoplasm of G EECs did not change significantly, but when 50100 渭 g / mL thymol acted on g EECs, the cell membrane broke down and the cells died. Moreover, the release of LDH in the cells with drug concentration 鈮，

本文編號：2162790