本文選題：美洲大蠊 + 提取物�。� 參考：《黑龍江畜牧獸醫(yī)》2017年07期

【摘要】：為了研究美洲大蠊提取物是否對大鼠血液和卵巢抗氧化指標產(chǎn)生影響,試驗使用成年雌性SD大鼠17只,適應飼養(yǎng)1周后,隨機分為3組,對照組(9 m L/kg,生理鹽水)5只、低劑量組(9 m L/kg,0.03 g/m L)6只、高劑量組(9 m L/kg,0.09 g/m L)6只,隔天灌胃1次,共飼養(yǎng)30 d。試驗結(jié)束后采集大鼠血液和卵巢檢測血清丙二醛(MDA)、一氧化氮(NO)含量和超氧化物歧化酶(SOD)、過氧化氫酶(CAT)、谷胱甘肽過氧化物酶(GSH-Px)活性以及卵巢SOD、GSH-Px、CAT基因表達的變化。結(jié)果表明:與對照組相比,高劑量組(P=0.085)、低劑量組(P=0.091)均有顯著降低血清中NO含量的趨勢;高劑量和低劑量的美洲大蠊提取物均顯著升高了大鼠血清中SOD的活性(P0.05),同時高劑量組還顯著降低了血清中MDA的含量(P0.05);此外,高劑量和低劑量組還顯著升高了卵巢SOD基因的表達(P0.05)。美洲大蠊提取物能升高大鼠血清抗氧化指標SOD的活性,降低了氧化應激指標NO和MDA的含量,同時還升高了卵巢SOD基因的表達量,說明其對大鼠卵巢具有一定的抗氧化應激功能。
[Abstract]:In order to study whether the extract of Blattella Americana had an effect on the antioxidant index of blood and ovary of rats, 17 adult female SD rats were used in the experiment. After being fed for one week, they were randomly divided into 3 groups: the control group was 9 mL / kg, and the normal saline was 5 rats. In the low dose group, 0. 03 g / mL 路kg ~ (-1) of 9 mL 路kg ~ (-1) 路kg ~ (-1) mg 路L ~ (-1) in the low dose group and in the high dose group (n = 6). The changes of serum malondialdehyde (MDA), nitric oxide (no), superoxide dismutase (SOD), catalase (catalase), glutathione peroxidase (GSH-Px) and the expression of GSH-PxX in ovary were measured after the experiment. The results showed that: compared with the control group, the high dose group and the low dose group all had the tendency of decreasing the serum no content significantly. Both high and low doses of Blattella Americana extract significantly increased the activity of SOD in serum of rats, and decreased the content of MDA in serum of high dose group. In addition, the high and low dose groups also significantly increased the expression of SOD gene in ovary. The extract of Blattella Americana could increase the activity of serum antioxidant index SOD, decrease the content of no and MDA, and increase the expression of SOD gene in ovary.
【作者單位】： 大理大學農(nóng)學與生物科學學院;
【基金】：云南省教育廳項目(2014C110Y) 大理大學博士科研啟動基金項目(KYBS201406)
【分類號】：S853.7

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