本文選題：酮病奶牛 + 硒�。� 參考：《動(dòng)物營(yíng)養(yǎng)學(xué)報(bào)》2016年12期

【摘要】：本試驗(yàn)旨在通過(guò)研究飼糧硒含量對(duì)酮病奶牛氧化應(yīng)激的緩解作用,并確定酮病奶牛對(duì)硒的最適需求量。采用完全隨機(jī)區(qū)組設(shè)計(jì),將40頭胎次為(3.43±0.87)胎、泌乳天數(shù)為(6.33±1.14)d、體重為(644±36)kg、平均泌乳量為(38.42±4.82)kg/d、血漿β-羥丁酸(BHBA)水平為(1.50±0.26)mmol/L的荷斯坦奶牛隨機(jī)分成4組,每組10頭牛。第1組為對(duì)照組,飼喂基礎(chǔ)飼糧(硒含量為0.15 mg/kg DM);第2、3、4組為試驗(yàn)組,在基礎(chǔ)飼糧中加入亞硒酸鈉(Na2Se O3)至飼糧硒含量分別達(dá)到0.30(試驗(yàn)組1)、0.45(試驗(yàn)組2)和0.60 mg/kg DM(試驗(yàn)組3)。試驗(yàn)分為3期,每期1周。試驗(yàn)每期結(jié)束后采集血樣,并在第2期結(jié)束后每組隨機(jī)選取5頭牛采集肝組織。結(jié)果表明:1)試驗(yàn)組酮病奶牛全血、肝臟中硒含量,肝臟中總抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性和谷胱甘肽過(guò)氧化物酶(GPx)活性以及GPx1與GPx4mRNA的相對(duì)表達(dá)水平均顯著高于對(duì)照組(P0.05),同時(shí)試驗(yàn)組2和3還顯著高于試驗(yàn)組1(P0.05)。2)試驗(yàn)組酮病奶牛肝臟中丙二醛(MDA)和過(guò)氧化氫(H2O2)含量均顯著低于對(duì)照組(P0.05),同時(shí)試驗(yàn)組2和3還顯著低于試驗(yàn)組1(P0.05)。3)試驗(yàn)組酮病奶牛血漿非酯化脂肪酸(NEFA)水平顯著低于對(duì)照組(P0.05),但血漿葡萄糖和BHBA水平與對(duì)照組相比無(wú)顯著差異(P0.05)。4)飼糧硒含量為0.45和0.60 mg/kg DM時(shí),酮病奶牛全血和肝臟中硒含量、肝臟抗氧化指標(biāo)和血漿能量代謝指標(biāo)均無(wú)顯著差異(P0.05)。由此得出,飼糧硒含量為0.45 mg/kg DM時(shí)可顯著改善酮病奶牛機(jī)體的抗氧化能力。
[Abstract]:The aim of this study was to study the effect of dietary selenium on oxidative stress and determine the optimum demand for selenium in ketogenic cows. A complete randomized block design was used to divide 40 Holstein cows with 3.43 鹵0.87, 6.33 鹵1.14 d, 644 鹵36 kg, 38.42 鹵4.82 kg / d, and 1.50 鹵1.50 鹵0.26)mmol/L plasma 尾 -hydroxybutyrate, respectively, into 4 groups, 10 cows in each group were randomly divided into 4 groups, 10 cows in each group were divided into 4 groups, with a mean lactating capacity of 38.42 鹵4.82 kg / d and a plasma 尾 -hydroxybutyrate of 1.50 鹵0.26)mmol/L. The first group was fed with basic diet (selenium content was 0.15 mg/kg DMN), the second group was treated with sodium selenite (Na2Se O 3), and the dietary selenium content reached 0.30 (test group 1: 0.45 (test group 2) and 0.60 mg/kg DM3 (test group 3). The trial was divided into 3 periods for one week each. Blood samples were collected at the end of each phase, and liver tissues were collected randomly from 5 cattle in each group after the end of phase 2. The results showed that the selenium content in whole blood and liver of ketosis cows in the test group was higher than that in the control group. The activities of T-AOC, SOD, Glutathione peroxidase (Glutathione peroxidase) and the relative expression of GPx1 and GPx4mRNA in liver were significantly higher than those in control group (P 0.05), while the activity of T-AOC, SOD and Glutathione peroxidase in experimental group 2 and 3 were significantly higher than those in control group. (1) the contents of malondialdehyde (MDAA) and hydrogen peroxide (H _ 2O _ 2) in the liver of ketosis cows were significantly lower than those in control group (P 0.05), and the levels of plasma non-esterified fatty acids (NEFAs) were significantly lower in test groups 2 and 3. The content of selenium in diet was 0.45 and 0.60 mg/kg DM, but there was no significant difference in plasma glucose and BHBA levels between the control group and the control group. There was no significant difference in selenium content in whole blood and liver, liver antioxidant index and plasma energy metabolism index in ketosis cows. It was concluded that dietary selenium content of 0.45 mg/kg DM could significantly improve the antioxidant capacity of ketosis cows.
【作者單位】： 江蘇農(nóng)牧科技職業(yè)學(xué)院寵物科技學(xué)院;上海光明荷斯坦牧業(yè)有限公司;
【基金】：生鮮牛乳中潛在生物性危害因子的評(píng)估研究[滬農(nóng)科攻字(2013)第3-1號(hào)] 江蘇高校品牌專(zhuān)業(yè)建設(shè)工程資助項(xiàng)目(PPZY2015C230)
【分類(lèi)號(hào)】：S858.23
，

本文編號(hào)：1951382