本文選題：豬 + let-��； 參考：《西南農(nóng)業(yè)學(xué)報》2017年05期

【摘要】：【目的】為研究miRNAs在仔豬免疫系統(tǒng)發(fā)育過程中的調(diào)控作用。【方法】本研究采用熒光定量PCR技術(shù)(Q-PCR)研究了不同泌乳期(0、3、7、14、21和28 d)的梅山豬和大約克夏豬母乳外泌體中l(wèi)et-7a和let-7f的差異表達。【結(jié)果】let-7a和let-7f在泌乳早期(0~3 d)的表達豐度明顯高于泌乳后期(7~28 d);品種間分析顯示梅山豬母乳外泌體中l(wèi)et-7a和let-7f在整個泌乳期(0~28d)的表達水平均明顯高于大約克夏豬。【結(jié)論】研究表明,let-7a和let-7f能夠正向調(diào)控仔豬免疫系統(tǒng)發(fā)育。本研究可為闡明母乳外泌體包被的miRNA在幼畜免疫系統(tǒng)發(fā)育過程中的具體調(diào)控機制提供基礎(chǔ)數(shù)據(jù)。
[Abstract]:[objective] to study the regulatory role of miRNAs in the development of immune system in piglets. [methods] in this study, the milk exocrine of Meishan pig and large Yorkshire pig at different lactation stages were studied by fluorescence quantitative PCR technique and Q-PCR. [results] the expression abundance of let-7a and let-7f in the early lactation stage was significantly higher than that in the late lactation stage (728 days), and the expression level of let-7a and let-7f in the breast milk exocrine of Meishan pig during the whole lactation period was significantly higher than that in the late lactation stage at 728 d). [results] the expression level of let-7a and let-7f in the breast milk exocrine of Meishan pig was significantly higher than that in the late lactation stage. [conclusion] the results showed that let-7a and let-7f could positively regulate the development of immune system in piglets. This study can provide basic data for elucidating the regulation mechanism of miRNA in the development of immune system in young animals.
【作者單位】： 四川省畜牧科學(xué)研究院動物遺傳育種四川省重點實驗室;
【基金】：國家自然科學(xué)基金(31301942) 四川省畜牧科學(xué)研究院基本科研業(yè)務(wù)費(SASA2014A08;SASA2015A01) 四川省科技支撐計劃(2014NZ0092)
【分類號】：S828
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本文編號：1936828