本文選題：番鴨 + MCR基因��； 參考：《江蘇農業(yè)科學》2017年10期

【摘要】：為探討黑素皮質素受體1(melanocortin-1 receptor,簡稱MC1R)基因對番鴨羽色的影響,采用PCR和直接測序法對番鴨MC1R基因編碼區(qū)進行多態(tài)性分析,比較不同羽色番鴨群體遺傳參數和基因型分布卡方檢驗。結果表明:在番鴨MC1R基因編碼區(qū)發(fā)現G274A、G279A、C485A突變,使第92處氨基酸由谷氨酸(E)變?yōu)橘嚢彼?K),第162處的氨基酸由蘇氨酸(T)變?yōu)樘於彼?N)。通過各個位點不同羽色番鴨群體基因型、等位基因頻率的比較分析,同時結合不同群體間基因型分布卡方檢測,發(fā)現在第279處的G-A突變中白羽番鴨僅有GG型,而其他3個攜帶大量黑羽的番鴨群體都以GA、AA型存在;同時全白番鴨與其他3個番鴨群體之間卡方值最大,推測此位點對番鴨的羽色影響較大。而從其他2個突變位點的數據分析推測,它們對番鴨的羽色幾乎沒有影響。研究結果為開展番鴨羽色研究和育種工作奠定了基礎。
[Abstract]:In order to study the effect of melanin receptor 1(melanocortin-1 receptor (MC1R) gene on the feather color of muscovy duck, PCR and direct sequencing were used to analyze the polymorphism of MC1R gene coding region in muscovy duck. The genetic parameters and genotype distribution of different feather muscovy duck populations were compared by chi-square test. The results showed that in the MC1R coding region of muscovy duck, the mutation of G274An G279AN C485A was found, which changed the 92nd amino acid from Glutamic acid E) to Lysine Ko, and the 162nd amino acid from threonine to aspartic acid. Based on the comparative analysis of genotype and allelic frequency of different loci in different populations of Muscovy duck, combined with Chi-square analysis of genotype distribution among different populations, it was found that White Muscovy Duck had only GG type in G-A mutation at 279. The other three muscovy duck populations with a large number of black feathers were GAAA-type, and the chi-square value between the whole white muscovy duck and the other three muscovy duck populations was the largest, which suggested that this locus had a great influence on the feather color of muscovy duck. From the data analysis of the other two mutation sites, it was inferred that they had little effect on the feather color of muscovy duck. The results laid a foundation for the research and breeding of Muscovy duck feather color.
【作者單位】： 江蘇農牧科技職業(yè)學院;揚州大學動物科學與技術學院;
【基金】：江蘇農牧科技職業(yè)學院院級課題(編號:NSF201502-2) 江蘇省'青藍工程'資助(編號:蘇教師[2016]15號)
【分類號】：S834

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