本文選題：豬偽狂犬病　切入點：豬傳染性胃腸炎　出處：《黑龍江畜牧獸醫(yī)》2017年01期

【摘要】：為了獲得高效價的豬偽狂犬病-傳染性胃腸炎二聯(lián)高免血清,試驗采用凍融法制備豬免疫血清,ELISA阻斷法、血凝試驗及血凝抑制試驗分別檢測各組豬偽狂犬病病毒、豬傳染性胃腸炎病毒血清抗體效價,比較試驗組及空白對照組兩種病原的血清抗體效價。將兩種病原抗體效價最高組血清分別置不同溫度保存不同時間檢測其抗體效價,探討最適保存條件。結(jié)果表明:S6組的兩種病原血清抗體效價最高,豬偽犬病病毒血清OD630值為0.352、豬傳染胃腸炎病毒血清抗體效價為1∶64;試驗組與空白對照組之間、S6組與其他試驗組之間抗體效價差異顯著。說明S6組為此次探討的豬偽狂犬病-傳染性胃腸炎二聯(lián)高免血清的最佳免疫程序,通過30 d的基礎(chǔ)免疫及10~15 d的強化免疫能獲得較高效價的豬偽狂犬病病毒、豬傳染性胃腸炎病毒血清抗體。-20℃和4℃分別適合豬偽狂犬病-傳染性胃腸炎二聯(lián)高免血清抗體液的長期和短期保存。
[Abstract]:In order to obtain the high titer of porcine pseudorabies and infectious gastroenteritis high immune serum, the porcine immune serum was prepared by freezing and thawing method. The hemagglutination test and hemagglutination inhibition test were used to detect pseudorabies virus in each group. The titer of serum antibody against infectious gastroenteritis virus was compared between the test group and the blank control group. The results showed that the antibody titers of the two pathogens were the highest in the two groups. The serum OD630 value of porcine pseudoviruses was 0.352, and the titer of serum antibody to porcine gastroenteritis virus was 1: 64. There were significant differences in antibody titers between S6 group and control group and other experimental groups, which indicated that S6 group was the pig pseudo virus in this study. The best immunization procedure for rabies and infectious gastroenteritis. The higher titer of porcine pseudorabies virus could be obtained by 30 days basic immunization and 1015 days intensive immunization. Serum antibody of porcine infectious gastroenteritis virus. -20 鈩，

本文編號：1670666