本文關(guān)鍵詞： 三氯卡班 乳腺上皮細胞 致癌 活性氧簇 DNA損傷　出處：《南京農(nóng)業(yè)大學(xué)學(xué)報》2017年05期 　論文類型：期刊論文

【摘要】：[目的]廣譜抗菌劑三氯卡班(TCC)在各種環(huán)境介質(zhì)和生物體中被頻繁檢出。為了研究TCC對動物的危害,觀察了TCC對正常小鼠乳腺上皮細胞(Ep H4-Ev)的急慢性損傷作用,評價其對乳腺上皮細胞的誘癌作用,為防止TCC在動物用品中的濫用提供理論依據(jù)。[方法]采用MTT法確定TCC的最佳工作濃度后,作用于Ep H4-Ev細胞,以48 h為1個藥物作用循環(huán),分別作用3和6個循環(huán)(命名為T3和T6細胞)后,使用MTT法檢測細胞增殖,流式細胞術(shù)檢測細胞內(nèi)活性氧簇(ROS)水平,Western blot法測定細胞內(nèi)磷酸化組蛋白(p-H2AX)的蛋白表達量,軟瓊脂克隆試驗檢測細胞非停泊性生長能力,細胞劃痕試驗測定細胞遷移能力,用此指標作為檢測TCC引起細胞癌變的指標。[結(jié)果]Ep H4-Ev細胞在0.195μmol·L-1TCC作用下,細胞活性最大,故0.195μmol·L-1為TCC的工作濃度;T3和T6細胞的細胞增殖水平、ROS水平、p-H2AX蛋白表達量及軟瓊脂上的細胞集落個數(shù)均顯著高于細胞對照;T6細胞的細胞劃痕之間的距離顯著小于細胞對照。[結(jié)論]TCC能夠誘導(dǎo)正常的Ep H4-Ev細胞發(fā)生癌變。
[Abstract]:[Objective] TCCs, a broad-spectrum antibacterial agent, were frequently detected in various environmental media and organisms. In order to study the harm of TCC to animals. To observe the acute and chronic injury effect of TCC on mammary epithelial cells of normal mice, and to evaluate its carcinogenic effect on mammary epithelial cells. In order to prevent TCC abuse in animal supplies to provide theoretical basis. [Methods] the optimal working concentration of TCC was determined by MTT method, and EpH4-Ev cells were treated with TCC for 48 h. After 3 and 6 cycles (named T3 and T6 cells), cell proliferation was detected by MTT assay, and intracellular reactive oxygen species (Ros) levels were detected by flow cytometry. The protein expression of phosphorylated histone (p-H2AX) was measured by Western blot assay, and the ability of non-moored growth was detected by soft Agar clone assay. The cell migration ability was measured by cell scratch test, which was used as a marker for the detection of cell carcinogenesis induced by TCC. [Results: the activity of EpH4-Ev cells was the highest under the treatment of 0.195 渭 mol 路L -1 TCC, so 0.195 渭 mol 路L -1 was the working concentration of TCC. The cell proliferation level of T3 and T6 cells and the expression of p-H2AX protein at Ros level and the number of colony on soft Agar were significantly higher than those in the control group. The distance between scratch of T 6 cells was significantly smaller than that of control cells. [Conclusion: TCC can induce carcinogenesis of normal EpH4-Ev cells.
【作者單位】： 南京農(nóng)業(yè)大學(xué)中獸醫(yī)學(xué)研究室;山東省平原縣畜牧獸醫(yī)局;
【基金】：國家自然科學(xué)基金項目(31572557) 國家公益性行業(yè)(農(nóng)業(yè))科研專項(2013030404,201403051) 江蘇高校優(yōu)勢學(xué)科建設(shè)工程項目(PAPD)
【分類號】：S852.3
【正文快照】： (1.Institute of Traditional Chinese Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;2.Animal Husbandry and Veterinary Bureau of Pingyuan Country,Shandong Province,Dezhou 253100,China)三氯卡班(triclocarban,TCC),其化學(xué)名稱為3,4,4-

【相似文獻】

相關(guān)期刊論文 前10條

1 王亨;韓超;邱昌偉;吳培福;齊長明;;體外培養(yǎng)奶牛乳腺上皮細胞的形態(tài)學(xué)觀察[J];中國獸醫(yī)雜志;2007年06期

2 楊櫟;李鍵;吳媛媛;田甜;畢琳;梁天宇;;綠色熒光蛋白基因在牦牛乳腺上皮細胞中表達的研究[J];西南民族大學(xué)學(xué)報(自然科學(xué)版);2009年03期

3 吳媛媛;李鍵;楊櫟;畢琳;田甜;;牦牛乳腺上皮細胞的體外培養(yǎng)的研究[J];西南民族大學(xué)學(xué)報(自然科學(xué)版);2009年04期

4 弓超;王凌云;周歡敏;;牛乳腺上皮細胞體外分離和培養(yǎng)[J];動物醫(yī)學(xué)進展;2011年07期

5 李吉霞;葛秀國;王文秀;張涌;;牛乳腺上皮細胞的分離培養(yǎng)[J];黑龍江畜牧獸醫(yī);2012年07期

6 符梅;熊顯榮;高川;李宇;王樹茂;李鍵;;麥洼牦牛乳腺上皮細胞系的建立及其生物學(xué)特性[J];西北農(nóng)業(yè)學(xué)報;2012年11期

7 郝明超;劉r，

本文編號：1460484