本文關鍵詞： 壽光雞 -羥色胺-N-乙酰基轉(zhuǎn)移酶基因(AANAT) 產(chǎn)蛋性狀 SNPs 關聯(lián)分析　出處：《農(nóng)業(yè)生物技術學報》2017年06期 　論文類型：期刊論文

【摘要】：褪黑激素(melatonin,Mel)分泌增加可阻礙家禽生殖器官的發(fā)育,降低家禽產(chǎn)蛋量。5-羥色胺-N-乙酰基轉(zhuǎn)移酶(arylalkylamine N-acetyltransferase,AANAT)是Mel合成過程中的限速酶。本實驗旨在研究AANAT基因5'調(diào)控區(qū)多態(tài)性及其與壽光雞(Gallus domesticus'Shouguang')產(chǎn)蛋性能的關系,采用直接測序法檢測了AANAT基因5'調(diào)控區(qū)的SNPs,并將檢測到的SNPs與壽光雞的產(chǎn)蛋性狀進行關聯(lián)分析。結(jié)果表明,AANAT基因5'控區(qū)共21個SNPs,其中10個低度多態(tài)、11個中度多態(tài)。3個中度多態(tài)位點對產(chǎn)蛋性能影響顯著(P0.05),其中-803位點能使開產(chǎn)日齡顯著提前,并能使前期(20~25周齡)產(chǎn)蛋數(shù)顯著增加;-561位點能使中期產(chǎn)蛋數(shù)顯著增加;-522位點能使后期(46~57周齡)產(chǎn)蛋數(shù)顯著增加;-561、-522位點能使產(chǎn)蛋總數(shù)顯著增加。綜上所述,AANAT基因5'調(diào)控區(qū)有多個SNPs對產(chǎn)蛋性狀影響顯著,可作為壽光雞產(chǎn)蛋性狀輔助選擇的有潛在利用價值的分子遺傳標記。
[Abstract]:The increased secretion of melatonin (melatonin) may hinder the development of reproductive organs in poultry. Decreased egg production. 5-hydroxytryptamine N-acetyltransferase (Arylalkylamine N-acetyltransferase). Aanat is a rate-limiting enzyme in the process of Mel synthesis. The aim of this study was to study the polymorphism of 5 'regulatory region of AANAT gene and its association with Shouguang chicken (. The relationship between Gallus domesticus and egg laying performance. SNPs of 5 'regulatory region of AANAT gene were detected by direct sequencing, and the results showed that the detected SNPs was associated with egg laying traits of Shouguang chicken. There were 21 SNPs in the 5'control region of AANAT gene, of which 10 were low polymorphic, 11 were moderate polymorphic, and 3 moderate polymorphic loci had significant effect on egg laying performance (P0.05). The -803 locus could significantly advance the first day and increase the number of eggs at 20 ~ 25 weeks. -561 locus could significantly increase the number of eggs laid in the middle stage. -522 locus could significantly increase the number of eggs laid at the later age of 46 ~ 57 weeks. The results showed that the total number of eggs was significantly increased at -561 ~ 522 locus. In conclusion, there were more than one SNPs in the 5 '-regulatory region of the AANAT gene, which had a significant effect on the egg laying traits. It can be used as a potentially useful molecular genetic marker for assisted selection of egg-laying traits in Shouguang Chicken.
【作者單位】： 山東農(nóng)業(yè)大學動物科技學院;
【基金】：山東省現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術體系項目(No.SDAIT-11-02)
【分類號】：S831.2
【正文快照】： 褪黑激素(melatonin,Mel)主要是由松果體和視網(wǎng)膜產(chǎn)生的一種吲哚類化合物(雷夢媛等,2015;趙雪丹等,2014),在哺乳動物及鳥類的多種組織廣泛分布,是光照信息同生殖系統(tǒng)協(xié)調(diào)關系中的重要信號(辛海瑞等,2016),可抑制下丘腦-垂體-性腺軸,使促性腺激素和促卵泡激素等的分泌減少、促

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