【摘要】：目的分析日本血吸蟲感染以及蟲源性糖蛋白果糖二磷酸醛縮酶(Sj FBPA)刺激后小鼠脾臟細胞中白介素4(IL-4)、白細胞分化抗原CD8a因子表達量的動態(tài)變化,并探討Sj FBPA與輔助型T細胞(Th2)免疫應答的關系。方法血吸蟲尾蚴感染C57小鼠10只,設正常對照組。感染8周后處死小鼠,取脾臟制備淋巴細胞懸液;用藻紅蛋白(PE)標記CD8a抗體,藻青蛋白(APC)標記IL-4抗體進行細胞染色;流式細胞儀檢測細胞因子表達量的變化。10只正常C57小鼠處死后取脾臟制成淋巴細胞懸液,加Sj FBPA蛋白體外刺激,按上述流程刺激方法檢測細胞。結果小鼠感染血吸蟲8周后脾細胞中IL-4、CD8a的表達量分別為(22.39±5.93)%、(11.34±2.15)%,正常對照組脾細胞中IL-4、CD8a的表達量分別為(1.39±0.52)%、(1.98±0.84)%,差異有統(tǒng)計學意義(P0.01)。Sj FBPA體外刺激小鼠脾細胞的IL-4、CD8a的表達量分別為(14.14±1.16)%、(8.99±0.81)%,正常對照組脾細胞中IL-4、CD8a的表達量分別為(1.86±0.93)%、(1.69±0.56)%,差異有統(tǒng)計學意義(P0.01)。結論 Sj FBPA在血吸蟲感染中具有誘導Th2免疫偏移的作用,樹突狀細胞作為提呈細胞參與該免疫反應。
[Abstract]:Objective to analyze the dynamic changes of expression of interleukin 4 (IL-4) and leukocyte differentiation antigen (CD8a) in spleen cells of mice after Schistosoma japonicum infection and (Sj FBPA) stimulation with insect-derived glycoprotein fructose diphosphate aldolase (Sj FBPA). To investigate the relationship between Sj FBPA and the immune response of helper T cells (Th2). Methods 10 C 57 mice infected with cercariae of Schistosoma japonicum were set up as normal control group. After 8 weeks of infection, the mice were killed and the spleen was taken to prepare lymphocyte suspensions, the CD8a antibody was labeled with phycoerythrin (PE) and the IL-4 antibody was stained with phycocyanin (APC). The expression of cytokines in 10 normal C57 mice was detected by flow cytometry. The spleen of 10 normal C57 mice was taken to make lymphocyte suspension and stimulated by Sj FBPA protein in vitro. Results the expression of IL-4,CD8a in spleen cells was (22.39 鹵5.93)% and (11.34 鹵2.15)% respectively after 8 weeks of schistosomiasis infection in mice, and the expression of IL-4,CD8a in splenocytes of normal control group was (1.39 鹵0.52)%. The expression of IL-4,CD8a was (14.14 鹵1.16)% and (8.99 鹵0.81)%, respectively. The expression of IL-4,CD8a was (1.86 鹵0.93)% and (1.69 鹵0.56)% in the normal control group, respectively. The difference was statistically significant (P0.01). Conclusion Sj FBPA can induce Th2 immune deviation in Schistosoma japonicum infection, and dendritic cells are involved in the immune response as presenting cells.
【作者單位】： 安徽醫(yī)科大學第一附屬醫(yī)院檢驗科;安徽人畜共患病研究所;
【基金】：國家自然科學基金(編號:81171606)
【分類號】：R532.21

【共引文獻】

相關期刊論文 前5條

1 王立富;孫希;吳忠道;;病原體感染中的Exosomes及其生物學功能[J];熱帶醫(yī)學雜志;2014年04期

2 田芳;胡雪莉;楊維平;錢莉;魏慧;劉浩;潘興元;季明春;;日本血吸蟲可溶性蟲卵抗原誘導B10細胞產(chǎn)生的研究[J];中國人獸共患病學報;2013年10期

3 柯雪丹;余傳信;宋麗君;菊池三惠子;平山謙二;王s，

本文編號：2360686