【摘要】：目的調(diào)查新疆阿勒泰山地蜱傳腦炎疫源地特征,分離鑒定蜱傳腦炎病毒。方法通過家畜體表?yè)旆ú杉纳?利用間接免疫熒光法檢測(cè)當(dāng)?shù)亟】等巳貉逯序鐐髂X炎病毒IgG抗體;通過將蜱研磨液接種實(shí)驗(yàn)小鼠進(jìn)行病原動(dòng)物分離;通過接種BHK-21細(xì)胞對(duì)蜱傳腦炎病毒進(jìn)行分離培養(yǎng);利用RT-PCR方法對(duì)病毒E蛋白基因片段進(jìn)行擴(kuò)增和測(cè)序,通過序列分析明確病毒系統(tǒng)進(jìn)化特征。結(jié)果新疆阿勒泰山地白哈巴地區(qū)分布有2種蜱,森林革蜱為優(yōu)勢(shì)種(55.6%),其次為邊緣革蜱(44.4%);當(dāng)?shù)厝巳候鐐髂X炎IgG抗體陽(yáng)性率5.31%(6/113);通過動(dòng)物試驗(yàn)和細(xì)胞分離培養(yǎng),從森林革蜱中分離出一株森林腦炎病毒;對(duì)病毒E蛋白基因序列的系統(tǒng)進(jìn)化分析表明,蜱傳腦炎病毒阿勒泰分離株屬于遠(yuǎn)東亞型。結(jié)論首次從病原學(xué)上證實(shí)新疆阿勒泰山地存在蜱傳腦炎疫源地,媒介為森林革蜱,病毒流行株為遠(yuǎn)東亞型。
[Abstract]:Objective to investigate the characteristics of tick-borne encephalitis foci in Altay Mountain, Xinjiang, and to isolate and identify tick-borne encephalitis virus. Methods the parasitic ticks were collected by the method of animal surface collection, the IgG antibody of tick-borne encephalitis virus was detected by indirect immunofluorescence method, and the pathogenic animals were isolated by inoculating ticks grinding fluid into experimental mice. The tick-borne encephalitis virus was isolated and cultured by inoculating BHK-21 cells, and the viral E protein gene fragment was amplified and sequenced by RT-PCR method, and the phylogenetic characteristics of the virus were identified by sequence analysis. Results there were two species of ticks in Baekhaba area of Altay Mountain, Xinjiang, the dominant species were forest ticks (55.6%), the next were the marginal ticks (44.4%), the positive rate of IgG antibody against tick-borne encephalitis in local population was 5.31% (6 / 113), and the positive rate was 5.31% (6 / 113). A forest encephalitis virus was isolated from the forest ticks, and the phylogenetic analysis of the E protein gene showed that the Acari encephalitis virus Altay isolate belonged to the far East subtype. Conclusion Acari encephalitis foci exist in Altay mountain area of Xinjiang for the first time, the vector is ticks, and the epidemic strain is far East subtype.
【作者單位】： 新疆軍區(qū)疾病預(yù)防控制中心;新疆軍區(qū)第十六醫(yī)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(No.U1303104)~~
【分類號(hào)】：R512.34

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