本文選題：結(jié)核分枝桿菌 + 基因分型��； 參考：《廣東醫(yī)學(xué)》2017年02期

【摘要】：目的探究徐州地區(qū)結(jié)核分枝桿菌的基因型分布特征及其與利福平耐藥基因突變形成的關(guān)系。方法選取徐州市傳染病醫(yī)院結(jié)核病實(shí)驗(yàn)室基因型毒株500株,其中含有經(jīng)羅氏比例法鑒定的耐利福平臨床分離株300株和利福平敏感200株,采用間隔區(qū)寡核苷酸分型(spoligotyping)分析其基因型結(jié)構(gòu)及其與利福平抗性基因的突變關(guān)系。結(jié)果徐州地區(qū)的結(jié)核分枝桿菌的基因分型主要為北京家族基因群(61.2%)和非北京家族基因群(38.8%),利福平耐藥和敏感組北京家族基因群的構(gòu)成比差異無統(tǒng)計(jì)學(xué)意義(醊2=0.09,P0.05);北京家族531位點(diǎn)、516位點(diǎn)以及531和526位點(diǎn)突變率分別為55.49%、10.99%和7.69%與非北京家族33.90%、21.19%和15.25%相比,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論徐州地區(qū)結(jié)核分枝桿菌基因型為北京家族基因群和非北京家族基因群,非北京家族基因群具有基因多態(tài)性,兩者與利福平耐藥率無明顯相關(guān),兩者在單531、單516以及531和526利福平耐藥位點(diǎn)突變率存在差異。
[Abstract]:Objective to investigate the genotypic distribution of Mycobacterium tuberculosis in Xuzhou and its relationship with rifampicin resistance gene mutation. Methods 500 strains of genotypic strains of tuberculosis were selected from Xuzhou Infectious Disease Hospital, including 300 strains of rifampin resistant clinical isolates and 200 strains of rifampicin sensitive strains identified by Roche scale method. The genotype structure and its relationship with rifampicin resistance gene were analyzed by spacer oligonucleotide typing (spoligotyping). Results the main genotypes of Mycobacterium tuberculosis in Xuzhou area were Beijing family gene group (61.2%) and non-Beijing family gene group (38.8%). There was no significant difference between rifampicin resistance group and sensitive group (P 0.05). The mutation rates of 531, 531 and 526 loci in Beijing family were 55.49% and 7.69%, respectively, compared with 33.90% and 15.25% in non-Beijing families. The difference was statistically significant (P0.05). Conclusion the genotypes of Mycobacterium tuberculosis in Xuzhou area are Beijing family gene group and non-Beijing family gene group. The gene polymorphism of non-Beijing family gene group has no significant correlation with rifampin resistance rate. The mutation rates of rifampin resistance loci in single 531, single 516 and 531 and 526 rifampicin were different between the two groups.
【作者單位】： 徐州市傳染病醫(yī)院結(jié)核二科;
【基金】：徐州市科技計(jì)劃項(xiàng)目(編號(hào):KC14SH055)
【分類號(hào)】：R52

【相似文獻(xiàn)】

相關(guān)期刊論文 前8條

1 王紅斌,翁小滿,溫艷,李桓英;巢式聚合酶鏈反應(yīng)檢測(cè)麻風(fēng)菌利福平耐藥基因的研究[J];中華檢驗(yàn)醫(yī)學(xué)雜志;2005年10期

2 陳紅兵,王仲元,張小剛,何秀云,董亞俊,張曉娟;中國(guó)部分地區(qū)耐利福平耐藥基因的檢測(cè)[J];廣東醫(yī)學(xué);2003年10期

3 趙玉玲;楊洪毅;馬曉光;石潔;李輝;趙東陽;;河南省耐多藥結(jié)核分枝桿菌利福平耐藥基因rpoB的突變特征[J];鄭州大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2012年02期

4 李曉榮;華樹成;;聚合酶鏈反應(yīng)-單鏈構(gòu)象多態(tài)性技術(shù)鑒別結(jié)核分枝桿菌利福平耐藥基因[J];海南醫(yī)學(xué)院學(xué)報(bào);2007年06期

5 李燕,楊正林,杜瓊,李明遠(yuǎn);單鏈構(gòu)象多態(tài)性分析技術(shù)檢測(cè)結(jié)核桿菌利福平耐藥基因[J];四川省衛(wèi)生管理干部學(xué)院學(xué)報(bào);2000年04期

6 牛占叢,張廣宇,王孟山,翟秉祥,劉延龍,李冀文;結(jié)核分枝桿菌利福平耐藥基因的檢測(cè)[J];臨床檢驗(yàn)雜志;1999年02期

7 張吉平;張小剛;何秀云;王仲元;金玉蓮;王慶;董亞俊;;結(jié)核分枝桿菌利福平耐藥基因突變的研究[J];中國(guó)預(yù)防醫(yī)學(xué)雜志;2006年05期

8 ;[J];;年期

相關(guān)會(huì)議論文 前2條

1 李邦印;;結(jié)核桿菌利福平耐藥基因檢測(cè)的研究進(jìn)展[A];耐多藥結(jié)核病診斷與治療研討會(huì)資料匯編[C];2005年

2 李鋒;李鳧堅(jiān);陳園園;王靜;;應(yīng)用基因芯片快速檢測(cè)結(jié)核分枝桿菌利福平耐藥基因的研究[A];2010年中國(guó)防癆協(xié)會(huì)臨床/基礎(chǔ)專業(yè)學(xué)術(shù)大會(huì)匯編[C];2010年

，

本文編號(hào)：2076513