本文選題：煙曲霉菌 + 生物被膜　； 參考：《廣西醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的：通過在體外復(fù)制煙曲霉菌(Aspergillus fumigates)的生物被膜(Biofilm, BF)模型，研究綠原酸(Chlorogenic acid，CRA)、異綠原酸(Isochlorogenic acid，IRC)對(duì)生物被膜的影響。 方法：選取臨床分離的煙曲霉菌構(gòu)建體外生物被膜模型，微量液基稀釋法測定最低抑菌濃度(Minimal inhibition concentration, MIC)，，將菌株分為空白對(duì)照組、實(shí)驗(yàn)組（CRA1024μg/ml、CRA512μg/ml、CRA256μg/ml、IRC1000μg/ml、IRC500μg/ml、IRC250μg/ml），掃描電子顯微鏡(Scanningelectron microscopy，SEM)、激光共聚焦掃描顯微鏡(Laser confocal scanningmicroscope， CLSM)定性觀察BF；結(jié)晶紫染色法半定量生物被膜量。 結(jié)果：37℃培養(yǎng)24h煙曲霉菌形成早期BF，48h形成成熟期BF。實(shí)驗(yàn)組煙曲霉菌BF均較其相應(yīng)空白對(duì)照組空洞、稀疏，但菌絲未見明顯減少，死活菌數(shù)也無明顯差別。CRA組早期結(jié)晶紫半定量結(jié)果分別為（1.05±0.19）、（1.14±0.26）、（1.16±0.14），IRC組為（1.18±0.06）、（1.21±0.06）、（1.26±0.04），均少于空白對(duì)照組（1.91±0.17），差異具有統(tǒng)計(jì)學(xué)意義（P0.05）；CRA組成熟期結(jié)晶紫半定量結(jié)果為（2.25±0.05）、（2.27±0.05）、（2.31±0.03），IRC組為（2.26±0.02）、（2.27±0.02）、（2.29±0.04），也均少于空白對(duì)照組（2.36±0.01），差異具有統(tǒng)計(jì)學(xué)意義（P0.05）。 結(jié)論：1、可以在體外建立煙曲霉菌生物被膜模型；2、CRA、IRC對(duì)體外煙曲霉菌BF形成有抑制作用。
[Abstract]:Objective: to study the effect of Lv Yuan acid Chlorogenic acidoglycan (Isochlorogenic acidogenic) on biofilm by replicating the biofilm model of Aspergillus fumigatesin vitro. Methods: a biofilm model was established in vitro by clinical isolates of Aspergillus fumigates.Methods: the biofilm model of Aspergillus fumigatesin vitro was established. Minimal inhibition concentration was determined by microdilution method. The strains were divided into blank control group. CRA1024 渭 g / ml CRA512 渭 g / ml CRA256 渭 g / ml IRC1000 渭 g / ml IRC500 渭 g / ml IRC250 渭 g / ml, scanning electron microscope (SEM), laser confocal scanning microscope (laser confocal scanning microscope), laser confocal scanning microscope (laser confocal scanning microscope), laser scanning microscope (CLSM), and semi-quantitative biofilm quantity of CRA 256 渭 g / ml. The BF of Aspergillus fumigatus in the experimental group was less than that in the control group, but the mycelium did not decrease obviously. 姝繪椿鑿屾暟涔熸棤鏄庢樉宸埆.CRA緇勬棭鏈熺粨鏅剁傳鍗婂畾閲忕粨鏋滃垎鍒負(fù)(1.05鹵0.19),(1.14鹵0.26),(1.16鹵0.14),IRC緇勪負(fù)(1.18鹵0.06),(1.21鹵0.06),(1.26鹵0.04),鍧囧皯浜庣┖鐧藉鐓х粍(1.91鹵0.17),宸紓鍏鋒湁緇熻瀛︽剰涔

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