本文選題：HBV + 宮內感染�。� 參考：《廣西醫(yī)科大學》2013年碩士論文

【摘要】：目的：研究乙型肝炎病毒宮內感染胎盤組織TLR4、HBD-2的表達。 方法：選擇2011年6月到2012年6月廣西醫(yī)科大學第一附屬醫(yī)院分娩的乙肝標志物HBsAg、HBeAg為陽性且肝功能正常的孕婦46例為試驗組,分娩后根據(jù)新生兒臍血清乙肝兩對半及HBVDNA結果,分為宮內感染組11例,宮內未感染組35例。乙肝標志物均為陰性的正常孕婦20例為對照組,采用免疫組織化學SP法檢測胎盤組織TLR4及HBD-2的表達。 結果：(1)孕婦血清HBsAg、HBeAg陽性的宮內感染率為23.9%。(2)免疫組化顯示HBsAg、HBeAg陽性孕婦及正常對照組的胎盤組織中均表達TLR4、HBD-2; HBsAg、HBeAg陽性孕婦的胎盤組織TLR4、HBD-2的表達均低于正常對照組；宮內感染組TLR4、HBD-2的表達均低于宮內未感染組及正常對照組(P0.05)。(3)TLR4陽性信號位于胎盤各層細胞上,包括滋養(yǎng)層細胞(TC)、蛻膜細胞(DC)、胎盤絨毛間質細胞(VMC)、絨毛毛細血管內皮細胞(VCMC),以胞漿分布為主。HBD-2陽性信號主要分布在胎盤滋養(yǎng)層細胞(TC),以胞漿分布為主。 結論：(1)HBsAg、HBeAg陽性孕婦胎盤組織TLR4、HBD-2表達均低于正常對照組(P0.05)。(2)宮內感染組胎盤組織的TLR4、HBD-2的表達均低于HBV宮內未感染組及正常對照組(P0.05)。TLR4、HBD-2可能參與HBV宮內感染的垂直調控,是孕婦宮內感染HBV的保護因素。
[Abstract]:Objective: to study the expression of TLR4 and HBD-2 in placenta of intrauterine hepatitis B virus infection. Methods: from June 2011 to June 2012, 46 pregnant women with positive HBsAg HBeAg and normal liver function were selected as the test group. According to the results of umbilical cord blood serum hepatitis B and HBVDNA, 46 pregnant women who were delivered in the first affiliated Hospital of Guangxi Medical University from June 2011 to June 2012 were selected as the test group. The patients were divided into intrauterine infection group (n = 11) and intrauterine infection group (n = 35). The expression of TLR4 and HBD-2 in placenta was detected by immunohistochemical SP method in 20 normal pregnant women with negative HBV markers. Results the intrauterine infection rate of HBsAg HBeAg positive in pregnant women was 23.90.The expression of TLR4HBD-2in HBsAg HBeAg positive pregnant women and normal control group was detected by immunohistochemistry, and the expression of TLR4HBD-2 in HBsAg HBeAg positive pregnant women was lower than that in normal controls. The expression of TLR4nHBD-2 in intrauterine infection group was lower than that in uninfected intrauterine group and normal control group. The positive signal of TLR4 was located on the cells of placenta. These include trophoblastic cells, decidual cells, placental chorionic stromal cells and chorionic capillary endothelial cells. The cytoplasmic and cytoplasmic distribution of HBD-2 positive signals were mainly observed in trophoblastic cells of the placenta and mainly in the cytoplasm of the trophoblastic cells. Conclusion the expression of TLR4HBeAg HBD-2 in placenta of pregnant women with positive HBeAg is lower than that in normal control group (P 0.05). The expression of TLR4 HBeAg in placenta of intrauterine infection group is lower than that of HBV group and normal control group. It may be involved in the vertical regulation of intrauterine HBV infection, and the expression of TLR4HBD-2 may be involved in the vertical regulation of intrauterine HBV infection. It is a protective factor for intrauterine infection of HBV in pregnant women.
【學位授予單位】：廣西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R512.62

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