本文選題：致瀉性大腸埃希菌　切入點：多重PCR　出處：《中國人獸共患病學報》2017年04期 　論文類型：期刊論文

【摘要】：目的掌握福建省腹瀉患者致瀉性大腸埃希菌(diarrheagenic Escherichia coli,DEC)的流行特征,為防控腹瀉病的流行和爆發(fā)提供依據(jù)。方法搜集2010-2015年延平區(qū)和永安市哨點醫(yī)院腹瀉病標本1 950份,接種于麥康凱(MaC)培養(yǎng)基,挑選平板上粉紅色、光滑型菌落,采用常規(guī)分離培養(yǎng)和分子診斷技術相結合的方法分離鑒定致瀉性大腸埃希菌,用SPSS 17.0對結果進行統(tǒng)計分析。結果致瀉性大腸埃希菌共檢出129株,總檢出率為6.62%;EPEC檢出65株,檢出率為3.33%,其中aEPEC 61株,tEPEC4株;ETEC和EAEC均檢出32株,檢出率為1.64%;未檢出EHEC和EIEC。男女DEC檢出率差異無統(tǒng)計學意義(P0.05);將腹瀉患者按照年齡分為5組,DEC檢出率分別為7.03%、4.60%、3.70%、7.28%和6.21%,各組EPEC、ETEC和EAEC檢出率差異無統(tǒng)計學意義(P0.05)。致瀉性大腸埃希菌(DEC)檢出率最高的月份為8月和9月;農村和城市DEC檢出率差異無統(tǒng)計學意義(P0.05);兩地區(qū)各類型DEC構成比差異無統(tǒng)計學意義(P0.05)。結論福建省DEC存在3種類型,以EPEC為主(其中aEPEC占絕對優(yōu)勢),8、9月是DEC高發(fā)月份,0~5歲嬰幼兒組及成人組是DEC的高發(fā)人群,合理使用抗生素治療DEC需要得到重視。
[Abstract]:Objective to study the epidemiological characteristics of diarrhea in diarrhea patients of Fujian Province, and to provide a basis for the prevention and control of diarrhoeal diseases. Methods 1,950 specimens of diarrhoeal diseases from 2010 to 2015 were collected in Yanping District and Qinling Hospital of Yongan City. Inoculated on Mac medium of Mac, selected pink and smooth colony on flat plate, isolated and identified cathartic Escherichia coli by conventional isolation, culture and molecular diagnostic techniques. Results A total of 129 strains of Escherichia coli were detected with SPSS 17.0. The total detection rate was 6.62 strains, and the detection rate was 3.33%. Among them, 61 strains of aEPEC were detected by aEPEC and 32 strains of EAEC were detected. There was no significant difference in the detection rate of EHEC and EIEC.There was no significant difference in the detection rate of DEC between men and women (P 0.05). According to age, the detectable rates of DEC were 7.03% and 6.21%, respectively. There was no significant difference in the detection rates of EAEC and EAEC between the two groups. The highest detection rate of Escherichia coli was in August and September. There was no significant difference in the detection rate of DEC between rural and urban areas (P 0.05), and there was no significant difference in DEC composition ratio between the two regions. Conclusion there are three types of DEC in Fujian Province. EPEC was the dominant factor (aEPEC was the dominant factor), September was the month of high incidence of DEC. The high incidence of DEC was in infants and adults. The rational use of antibiotics in the treatment of DEC should be paid more attention to.
【作者單位】： 福建省疾病預防控制中心;福建省人獸共患病研究重點實驗室;
【基金】：福建省醫(yī)學創(chuàng)新課題(No.2011-CXB-19)資助~~
【分類號】：R446.5;R516
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本文編號：1604984