【摘要】：目的 人副流感病毒1型能夠引起以哮吼為主要癥狀的呼吸道感染,是幼兒呼吸道感染而住院治療的主要病原體之一,同時又可以累及成人。仙臺病毒,又稱鼠源副流感病毒1型,仙臺病毒的HN糖蛋白與人源副流感病毒1型HN蛋白序列高度同源,并且是仙臺病毒主要的抗原蛋白。因此,本研究用構(gòu)建的仙臺病毒HN核酸疫苗免疫幼鼠,評價疫苗的免疫效果,以尋求預防副流感病毒1型感染的新方法。 方法 用去內(nèi)毒素處理后的仙臺病毒HN核酸疫苗(pcDNA-HN)肌肉注射免疫4-6周齡幼鼠,加強免疫兩次,4周后用仙臺病毒攻擊小鼠。以滅活的仙臺病毒疫苗為陽性對照、pcDNA3和生理鹽水作為陰性對照,采用1μg/0.1ml和5μg/0.1ml兩個濃度的仙臺病毒HN基因核酸疫苗做為觀察對象。觀察小鼠的肺組織病理學變化,通過B細胞和T細胞增殖反應、IFN-γ含量測定、血凝抑制試驗、血清IgG抗體的測定及紅細胞免疫功能測定等多項指標評價仙臺病毒HN基因核酸疫苗的免疫應答效果。 結(jié)果 pcDNA-HN疫苗組肺指數(shù)顯著低于生理鹽水組、肺組織病理學無明顯改變;與pcDNA3和生理鹽水組比較,疫苗組可明顯增強T、B淋巴細胞增殖能力,兩者
[Abstract]:Objective Human parainfluenza virus type 1 can cause respiratory tract infection with roar as the main symptom. It is one of the main pathogens of respiratory tract infection in young children and can involve adults at the same time. Sendai virus, also known as mouse parainfluenza virus type 1, Sendai virus HN glycoprotein and human parainfluenza virus type 1 HN protein sequence is highly homologous, and is the main antigen protein of Sendai virus. Therefore, the constructed Sendai virus HN nucleic acid vaccine was used to immunize young mice and evaluate the immune effect of the vaccine in order to find a new method to prevent parainfluenza virus type 1 infection. Methods Sendai virus HN nucleic acid vaccine (pcDNA-HN) was intramuscular injected into young mice aged 4 weeks for 6 weeks and immunized twice. Four weeks later, the mice were attacked with Sendai virus (Sendai virus). The mice were immunized with Sendai virus (Sendai virus) intramuscular injection of Sendai virus nucleic acid vaccine (Sendai). The inactivated Sendai virus vaccine was used as positive control, pcDNA3 and saline as negative control, and 1 渭 g / 0.1ml and 5 渭 g / 0.1ml HN gene nucleic acid vaccine were used as observation objects. The pathological changes of lung tissue in mice were observed. the proliferation of B cells and T cells, the content of IFN- 緯 and the inhibition test of hemagglutination were measured. The determination of serum IgG antibody and erythrocyte immune function were used to evaluate the immune response effect of Sendai virus HN gene nucleic acid vaccine. Results the lung index of pcDNA-HN vaccine group was significantly lower than that of saline group, and there was no significant change in lung pathology. Compared with pcDNA3 and saline group, the proliferation ability of T and B lymphocytes in vaccine group was significantly enhanced.
【學位授予單位】：天津醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R392

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