【摘要】： 位于腦干網(wǎng)狀結(jié)構(gòu)抑制區(qū)的巨細(xì)胞網(wǎng)狀核(NRG)是一個(gè)功能復(fù)雜的核團(tuán)，其無論是對(duì)軀體運(yùn)動(dòng)，還是對(duì)內(nèi)臟活動(dòng)都有影響。NRG對(duì)軀體運(yùn)動(dòng)的抑制性中樞調(diào)控作用，在神經(jīng)系統(tǒng)電生理學(xué)及形態(tài)學(xué)研究中已得到充分證明，但該核團(tuán)與內(nèi)臟運(yùn)動(dòng)的聯(lián)系尚缺乏全面、系統(tǒng)的資料。本實(shí)驗(yàn)擬從形態(tài)學(xué)上對(duì)巨細(xì)胞網(wǎng)狀核的功能聯(lián)系加以補(bǔ)充說明。 目的 1．觀察大鼠巨細(xì)胞網(wǎng)狀核與腦神經(jīng)運(yùn)動(dòng)核的直接纖維聯(lián)系。 2．比較兩種神經(jīng)示蹤劑WGA—HRP和DiI的標(biāo)記效果。 方法 1．用微量注射法將碳素墨水注入一側(cè)巨細(xì)胞網(wǎng)狀核，進(jìn)行尼氏染色，觀察NRG的定位準(zhǔn)確性。 2．將WGA—HRP注入一側(cè)NRG，采用TMB—ST法成色，光學(xué)顯微鏡下觀察注射部位是否準(zhǔn)確及標(biāo)記情況。 3．將DiI注入一側(cè)NRG，取材，熒光顯微鏡下觀察注射部位及標(biāo)記情況。 結(jié)果 1．一般軀體運(yùn)動(dòng)核：動(dòng)眼神經(jīng)核、滑車神經(jīng)核雙側(cè)可見逆行標(biāo)記細(xì)胞及順行神經(jīng)纖維終末，逆行標(biāo)記細(xì)胞同側(cè)多于對(duì)側(cè)；展神經(jīng)核未見標(biāo)記；舌下神經(jīng)核雙側(cè)也看到逆行標(biāo)記細(xì)胞及神經(jīng)纖維終末。 2．一般內(nèi)臟運(yùn)動(dòng)核：下泌涎核、迷走神經(jīng)背核雙側(cè)均見少量逆行標(biāo)記細(xì)胞及順行神經(jīng)纖維終末；上泌涎核未見標(biāo)記。 3．特殊內(nèi)臟運(yùn)動(dòng)核：面神經(jīng)核、疑核、三叉神經(jīng)運(yùn)動(dòng)核雙側(cè)均發(fā)現(xiàn)逆行標(biāo)記細(xì)胞及順行纖維末梢。 4．WGA—HRP和DiI標(biāo)記的結(jié)果分布相似，不同的是，，DiI在下泌涎核未顯示出標(biāo)記，且在其他核團(tuán)標(biāo)記的細(xì)胞數(shù)目沒有WGA—HRP標(biāo)記的多。 結(jié)論 1．大鼠巨細(xì)胞網(wǎng)狀核與絕大多數(shù)腦神經(jīng)運(yùn)動(dòng)核有纖維聯(lián)系，且投射方向?yàn)殡p向。 2．DiI在成年動(dòng)物活體腦中的標(biāo)記效果不及WGA—HRP理想。
[Abstract]:(NRG) in the reticular nucleus of giant cells located in the inhibitory region of the reticular structure of the brain stem is a complex nuclear mass, which affects both the somatic movement and the visceral activity. The inhibitory central regulation of NRG on the somatic movement. It has been fully proved in the electrophysiology and morphology of nervous system, but the connection between the nucleus and visceral movement is still lack of comprehensive and systematic data. In this study, the functional connections of the reticular nucleus of giant cells were elucidated morphologically. Objective 1. To observe the direct fibrous connection between the reticular nucleus of giant cells and the motor nucleus of the brain. 2. The labeling effects of WGA-HRP and DiI were compared. Methods 1. Carbon ink was injected into one side of giant cell reticular nucleus by microinjection, and the localization accuracy of NRG was observed. 2. 2. The injection of WGA-HRP into one side of NRG, was colored by TMB-ST method, and the accuracy and labeling of the injection site were observed under optical microscope. 3. DiI was injected into one side of NRG, and the injection site and labeling were observed under fluorescence microscope. Results 1.General somatomotor nucleus: retrograde labeled cells and anterograde nerve fiber terminals were found on both sides of oculomotor nucleus, trochlear nucleus, ipsilateral retrograde labeled cells were more than contralateral, abductor nucleus was not labeled. Retrograde labeled cells and nerve fiber terminals were also seen on both sides of hypoglossal nucleus. 2. In general visceral motor nucleus, a few retrograde labeled cells and antegrade nerve terminals were found in the lower salivary nucleus and the dorsal nucleus of vagus, but no labeling was found in the upper salivary nucleus. 3. Special visceral motor nuclei: retrograde labeled cells and antegrade fibrous endings were found in the facial nucleus, the nucleus suspect and the motor nucleus of the trigeminal nerve. The results of 4.WGA-HRP and DiI labeling were similar, the difference was that DiI showed no markers in the lower salivary nucleus, and the number of cells labeled in other nuclei was not more than that labeled by WGA-HRP. Conclusion 1. The reticular nucleus of giant cells in rats has fibrous connections with most of the motor nuclei of the brain, and the projection direction is bidirectional. 2. The labeling effect of 2.DiI in the living brain of adult animals was not as good as that of WGA-HRP.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：R322

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王子仁,R.L.麥耶;河豚毒素對(duì)金魚再生的視網(wǎng)膜頂蓋投射模式的影響[J];動(dòng)物學(xué)報(bào);1995年03期

2 陳德英,蔡文琴,劉建軍;大鼠中樞神經(jīng)系統(tǒng)全程連續(xù)切片顯示神經(jīng)核團(tuán)的新方法[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2001年12期

3 包新民,舒斯云,牛東濱;大鼠延髓和腦橋中生長(zhǎng)抑素樣神經(jīng)元對(duì)咽肌前運(yùn)動(dòng)神經(jīng)元的調(diào)控[J];第一軍醫(yī)大學(xué)學(xué)報(bào);1997年04期

4 宋一志;陸濤;李莉;丁衛(wèi)國;馬育平;高秀來;;大鼠未固定神經(jīng)組織DiI熒光示蹤方法的研究[J];解剖學(xué)研究;2006年02期

5 王少錦,孫彥輝,趙志國,楊天祝;大鼠腦立體定向手術(shù)校正的三種方法[J];河北醫(yī)科大學(xué)學(xué)報(bào);2001年02期

6 孫松濤,王波,徐林,吳夕;SPR對(duì)痙攣性腦癱眼部斜視的療效及其機(jī)理[J];河北醫(yī)學(xué);2001年02期

7 李戩,徐旭東,屠建琪,劉曉安;鼠大巨細(xì)胞網(wǎng)狀核α部注入6-羥基多巴胺對(duì)痛閾的影響[J];濟(jì)寧醫(yī)學(xué)院學(xué)報(bào);1992年01期

8 蔡淑頻,張書琴;家兔延髓巨細(xì)胞網(wǎng)狀核內(nèi)突觸復(fù)合體的超微結(jié)構(gòu)[J];解剖科學(xué)進(jìn)展;2003年01期

9 舒斯云;;貓網(wǎng)狀脊髓束的起源和終止——HRP法研究[J];解剖學(xué)報(bào);1981年03期

10 鄧錦波;于東明;吳萍;;Dil散射標(biāo)記神經(jīng)元及神經(jīng)膠質(zhì)細(xì)胞技術(shù)介紹[J];解剖學(xué)報(bào);2006年05期

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