【摘要】：結(jié)核病(Tuberculosis,TB)是由結(jié)核分枝桿菌(Mycobacterium tuberculosis,MTB)所致以呼吸系統(tǒng)感染為主的慢性傳染病,據(jù)WHO估計,目前全世界約有1/3人口感染MTB,每年有1000萬新發(fā)患者和300萬患者死亡。貧窮、人口增加、流動人口增多、耐藥MTB增多及艾滋病流行等使TB問題雪上加霜。我國是全球22個TB高負(fù)擔(dān)國家之一,現(xiàn)有MTB感染人數(shù)已達(dá)4億,傳染性TB患者達(dá)到200萬�？ń槊�(BCG)雖被廣泛用于預(yù)防TB,但其效果仍不夠滿意。研究認(rèn)為BCG可預(yù)防并減輕兒童的嚴(yán)重TB,但對成人TB的預(yù)防作用從0到80%不等。導(dǎo)致此現(xiàn)象的原因在于BCG菌株的變異使保護(hù)性抗原丟失、人群間遺傳或營養(yǎng)的差異及環(huán)境等因素的影響。鑒于BCG自身的不足,以及當(dāng)前TB流行的嚴(yán)重性,研究新的疫苗用于TB的預(yù)防已成為當(dāng)前國內(nèi)外研究熱點。 MTB早期培養(yǎng)濾液蛋白(CFP)中的ESAT6和CFP10是重要的保護(hù)性抗原。本研究先后構(gòu)建了融合表達(dá)ESAT6和CFP10蛋白的亞單位疫苗、基因疫苗以及重組恥垢分枝桿菌(mycobacterium smegmatis,M.S)疫苗,并比較了各自誘發(fā)的細(xì)胞免疫應(yīng)答水平和對感染小鼠的保護(hù)力,
[Abstract]:Tuberculosis (Tuberculosis,TB) is a chronic infectious disease mainly caused by respiratory infection caused by Mycobacterium tuberculosis (Mycobacterium tuberculosis,MTB). According to WHO estimates, about one-third of the world's population is infected with MTB,. 10 million new cases and 3 million deaths occur every year. Poverty, population growth, increased floating population, increased drug-resistant MTB and the AIDS epidemic make the TB problem worse. China is one of the 22 countries with high burden of TB in the world. The number of people infected with MTB has reached 400 million and the number of patients with infectious TB has reached 2 million. BCG (BCG) is widely used to prevent TB, but its effect is not satisfactory. The study suggests that BCG can prevent and reduce severe TB, in children, but the preventive effect on adult TB varies from 0 to 80%. The causes of this phenomenon are the loss of protective antigen due to the variation of BCG strains, the genetic or nutritional differences among populations and the influence of environmental factors. In view of the deficiency of BCG itself and the severity of the current TB epidemic, the research on the application of new vaccines to the prevention of TB has become a hot research topic at home and abroad. ESAT6 and CFP10 in MTB early culture filtrate protein (CFP) are important protective antigens. In this study, subunit vaccines, gene vaccines and recombinant Mycobacterium smegmatis (mycobacterium smegmatis,M.S) vaccines expressing ESAT6 and CFP10 proteins were constructed successively, and the cellular immune response levels and the protective effects on infected mice were compared.
【學(xué)位授予單位】：第四軍醫(yī)大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2005
【分類號】：R392

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 謝婷;李文桂;;結(jié)核分枝桿菌CFP10疫苗研究進(jìn)展[J];中國病原生物學(xué)雜志;2007年03期

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1 簡文;屋塵螨抗原Der p2重組恥垢分枝桿菌口服疫苗的制備及其實驗研究[D];第四軍醫(yī)大學(xué);2008年

相關(guān)碩士學(xué)位論文 前1條

1 謝婷;結(jié)核分枝桿菌重組Bb-ESAT-6-MPT64疫苗保護(hù)力及其免疫機(jī)制研究[D];重慶醫(yī)科大學(xué);2008年

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本文編號：2375840