發(fā)布時(shí)間：2018-11-19 12:42

【摘要】：在生殖、免疫、炎癥的一些關(guān)鍵性過(guò)程中,分子水平上存在相似的特征。黏附分子L-選擇素與其配體的結(jié)合介導(dǎo)了免疫系統(tǒng)的淋巴細(xì)胞歸巢,及炎癥過(guò)程白細(xì)胞與內(nèi)皮細(xì)胞的黏附,并發(fā)揮著信號(hào)分子作用。近年研究者發(fā)現(xiàn),參與免疫和炎癥過(guò)程的重要細(xì)胞黏附分子L-選擇素及其配體也存在于哺乳動(dòng)物著床期母胎界面上。Genbacev等發(fā)現(xiàn)L-選擇素與其配體的結(jié)合可能構(gòu)成人類(lèi)胚胎著床的起始步驟。著床窗口期,胚胎表面的L-選擇素和母體子[諛-選擇素的寡糖配體似乎都呈現(xiàn)上調(diào)趨勢(shì)。L-選擇素配體至少包括四種分子:CD34、粘膜區(qū)素細(xì)胞粘附分子-1(MAdCAM-1)、依賴(lài)糖基化的細(xì)胞粘附分子-1(GlyCAM-1)、Sgp200;由于最早發(fā)現(xiàn)于小鼠淋巴結(jié)高內(nèi)皮靜脈,故將它們統(tǒng)稱(chēng)為周?chē)馨徒Y(jié)區(qū)素(PNAd)。L-選擇素識(shí)別硫酸化形式的配體,N-乙酰葡萄糖胺-6-轉(zhuǎn)磺酶(GlcNAc6ST)在功能型L-選擇素配體生成過(guò)程中發(fā)揮重要作用。 盡管以往的研究發(fā)現(xiàn)了人類(lèi)母胎界面上存在L-選擇素和其配體,但有關(guān)早期胚胎發(fā)育過(guò)程中L-選擇素的表達(dá)規(guī)律、周期性變化的子[諛ど螸-選擇素配體表達(dá)的時(shí)間、空間模式、妊娠建立后它們又將如何變化、它們的表達(dá)水平是否與妊娠成功率有關(guān),這一系列問(wèn)題目前尚未見(jiàn)報(bào)道。盡管體外研究表明這對(duì)配體一受體確實(shí)能夠介導(dǎo)細(xì)胞之間的黏附,但尚無(wú)體內(nèi)實(shí)驗(yàn)證明它們?cè)谂咛ブ仓械淖饔�。為了深入探討L-選擇素系統(tǒng)在胚胎著床中的功能和影響,我們檢測(cè)人類(lèi)母胎界面L-選擇素及其配體表達(dá)的時(shí)間規(guī)律、位置特征,分析分泌期子[諛-選擇素配體(PNAd)各組成分子以及GlcNAc6ST mRNA水平與體外
[Abstract]:There are similar characteristics at the molecular level in some key processes of reproduction, immunity, and inflammation. The binding of the adhesion molecule L- selectin to its ligand mediates the homing of lymphocytes in the immune system and the adhesion of leukocytes to endothelial cells during inflammation and acts as a signaling molecule. In recent years, researchers have found that L- selectin and its ligands, which are important cell adhesion molecules involved in immune and inflammatory processes, also exist at mammalian maternal and fetal interfaces. Genbacev et al found that the binding of L- selectin to its ligands may constitute the initial step of human embryo implantation. L- selectin and mother on embryo surface during implantation window period [? Flat-selectin oligosaccharide ligands appear to be on an upward trend. Lselectin ligands contain at least four molecules: CD34, mucosal adhesion molecule-1 (MAdCAM-1), glycosylated cell-adhesion molecule-1 (GlyCAM-1), and glycosylated oligosaccharide ligands. Sgp200; Because they were first found in the high endothelial veins of the lymph nodes of mice, they were collectively referred to as the ligand of (PNAd). L- selectin recognition of sulfated form in peripheral lymph nodes. N-acetyl glucosamine-6-transferase (GlcNAc6ST) plays an important role in the formation of functional L-selectin ligands. Although previous studies have found the presence of L- selectin and its ligands at the human maternal and fetal interface, the expression of L- selectin during early embryonic development is related to the periodic variation of the L- selectin gene [? The time, spatial pattern, how they will change after pregnancy and whether their expression level is related to the success rate of pregnancy have not been reported. Although in vitro studies have shown that these ligands and receptors can indeed mediate cell adhesion, no in vivo studies have demonstrated their role in embryo implantation. In order to study the function and influence of L- selectin system in embryo implantation, we detected the expression of L- selectin and its ligand in human maternal and fetal interface, and analyzed the secretory phase of L- selectin and its ligand. Flat- selectin ligand (PNAd) components, levels of GlcNAc6ST mRNA and in vitro
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2005
【分類(lèi)號(hào)】：R321

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 范衡宇,白玉妍,楊增明;胚泡著床窗口的分子調(diào)控[J];生物化學(xué)與生物物理進(jìn)展;2001年01期

，

本文編號(hào)：2342347